LOCUS       B892p35S-CherD-o        4294 bp    DNA     circular SYN 04-OCT-2017
DEFINITION  Ligation of mCherry-Mlu-Sal* into B589p35s-mGFPD-ocs*
ACCESSION   B892p35S-CherD-o
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B589p35s-mGFPD-ocs*
               Fragment ends: SalI and MluI
               Fragment size: 3563
            Insert molecule:  mCherry-Mlu-Sal*
               Fragment ends: MluI and SalI
               Fragment size: 731
            Sequencing primers:
            35S	GCACAATCCCACTATCCTT
FEATURES             Location/Qualifiers
     misc_feature    144..562
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S-Pro"
                     /SECDescr="Promotor-Region"
     misc_feature    473..562
                     /gene="Minimal promotor"
                     /SECDrawAs="Label"
     CDS             590..1300
                     /gene="mCherry"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCherry"
     misc_feature    1309..1504
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    2320..3180
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3223..3939
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa
      181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt
      241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
      361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt
      421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tggtgtccaa
      601 gggcgaggag gacaacatgg ccatcattaa ggagttcatg cgcttcaaag tgcacatgga
      661 aggaagcgtt aacggccacg agttcgagat cgagggtgag ggagagggtc gtccctacga
      721 aggaacccag actgctaagc tcaaagtcac caagggcgga ccattgcctt tcgcctggga
      781 catcttgtcc ccgcaattta tgtacggctc taaggcatac gtgaagcacc ccgccgatat
      841 tccagactac cttaagctct cattccccga gggtttcaag tgggagaggg tcatgaactt
      901 cgaggacgga ggcgtggtta ccgtcacaca ggactccagc ttgcaggatg gcgagttcat
      961 ctacaaggtg aagctccgcg ggaccaattt cccatcggac ggccctgtca tgcagaagaa
     1021 gactatggga tgggaggctt ccagtgaaag aatgtatccc gaggacggcg ccctgaaggg
     1081 agagatcaag caacgcctca agttgaagga cggcggtcat tacgacgcgg aagtgaagac
     1141 gacctacaag gccaagaagc cggttcagct tccaggcgcc tacaacgtca acatcaagct
     1201 ggacatcacc agccacaacg aggactacac tatcgtggag cagtacgagc gtgctgaggg
     1261 aaggcactcc accggaggca tggacgagct ttacaagtga ctcgtcgacc ctgctttaat
     1321 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
     1381 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
     1441 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
     1501 gtcctcgaca ggccttaagg gccagatctt gggcccggta cccaattcgc cctatagtga
     1561 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1621 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1681 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta
     1741 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     1801 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     1861 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     1921 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     1981 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2041 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2101 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2161 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2221 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2281 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2341 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2401 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2461 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2521 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2581 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2641 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2701 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2761 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2821 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2881 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2941 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3001 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3061 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3121 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3181 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3241 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3301 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3481 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3781 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3841 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3901 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3961 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4021 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4081 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4141 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4201 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4261 gctttacact ttatgcttcc ggctcgtatg ttgt
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