LOCUS       B893p35S-CheriD-        4483 bp    DNA     circular SYN 04-OCT-2017
DEFINITION  Ligation of cheri-Mlu-Sal* into B892p35S-CherD-ocs*
ACCESSION   B893p35S-CheriD-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B892p35S-CherD-ocs*
               Fragment ends: SalI and MluI
               Fragment size: 3563
            Insert molecule:  cheri-Mlu-Sal*
               Fragment ends: MluI and SalI
               Fragment size: 920
            Sequencing primers:
            35S	GCACAATCCCACTATCCTT
FEATURES             Location/Qualifiers
     misc_feature    144..562
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S-Pro"
                     /SECDescr="Promotor-Region"
     misc_feature    473..562
                     /gene="Minimal promotor"
                     /SECDrawAs="Label"
     CDS             590..969
                     /gene="mCherry'"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCherry"
     CDS             987..1156
                     /gene="Int"
                     /product="ST-LS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1168..1489
                     /gene="'mCherry"
                     /codon_start=2
                     /translation="GTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKD
                     GGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMD
                     ELYK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCherry"
     misc_feature    1498..1693
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    2509..3369
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3412..4128
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa
      181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt
      241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
      361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt
      421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tggtgtccaa
      601 gggcgaggag gacaacatgg ccatcattaa ggagttcatg cgcttcaaag tgcacatgga
      661 aggaagcgtt aacggccacg agttcgagat cgagggtgag ggagagggtc gtccctacga
      721 aggaacccag actgctaagc tcaaagtcac caagggcgga ccattgcctt tcgcctggga
      781 catcttgtcc ccgcaattta tgtacggctc taaggcatac gtgaagcacc ccgccgatat
      841 tccagactac cttaagctct cattccccga gggtttcaag tgggagaggg tcatgaactt
      901 cgaggacgga ggcgtggtta ccgtcacaca ggactccagc ttgcaggatg gcgagttcat
      961 ctacaaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt
     1021 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc
     1081 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa
     1141 atttgttgat gtgcaggtga agctccgcgg gaccaatttc ccatcggacg gccctgtcat
     1201 gcagaagaag actatgggat gggaggcttc cagtgaaaga atgtatcccg aggacggcgc
     1261 cctgaaggga gagatcaagc aacgcctcaa gttgaaggac ggcggtcatt acgacgcgga
     1321 agtgaagacg acctacaagg ccaagaagcc ggttcagctt ccaggcgcct acaacgtcaa
     1381 catcaagctg gacatcacca gccacaacga ggactacact atcgtggagc agtacgagcg
     1441 tgctgaggga aggcactcca ccggaggcat ggacgagctt tacaagtgac tcgtcgaccc
     1501 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg
     1561 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca
     1621 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat
     1681 ttactgattg tcctcgacag gccttaaggg ccagatcttg ggcccggtac ccaattcgcc
     1741 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1801 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1861 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1921 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     1981 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2041 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2101 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2161 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2221 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2281 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2341 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     2401 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2461 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2521 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2581 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2641 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2701 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2761 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2821 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2881 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2941 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3001 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3061 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3121 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3181 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3241 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3301 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3361 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4201 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4261 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     4441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgt
//
