LOCUS       B982p935sGFP           10390 bp    DNA     circular SYN 04-OCT-2017
DEFINITION  Ligation of NptGFP-Xho-Mlu* into B414p7o-LH*
ACCESSION   B982p935sGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B414p7o-LH*
               Fragment ends: MluI and XhoI
               Fragment size: 8426
            Insert molecule:  NptGFP-Xho-Mlu*
               Fragment ends: XhoI and MluI
               Fragment size: 1964
            Primer for gene synthesis
            NptXho	cccggtCTCGAGtcctccataacaATGGGAGCATGGATTGA
            GFPMlu	CCGCacgcGTCACTTGTAGAGTTCATC
            Sequencing primers:
            35S	GCACAATCCCACTATCCTT
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..1043
                     /gene="NptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1044..1230
                     /gene="Int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1231..1734
                     /gene="NptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1786..2164
                     /gene="GFPm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2165..2353
                     /gene="Int"
                     /product="ST-LS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2354..2691
                     /gene="GFPm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2701..2991
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3167..3190
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3725..4422
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4459..4718
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4971..8676
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8860..9870
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aacaatggga gcatggattg agcaggatgg cctccatgct
      781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt
      841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg
      901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg
      961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga
     1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat
     1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac
     1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg
     1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact
     1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct
     1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc
     1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc
     1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg
     1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt
     1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttcccggta
     1741 ccgccccgtc cggtcctgcc cgtcaccgag atctccggag aattcatggg caagggcgag
     1801 gaactgttca ctggcgtggt cccaatcctg gtggaactgg atggtgatgt gaacgggcac
     1861 aagttctccg tcagcggaga gggtgaaggt gatgccacct acggaaagct caccctgaag
     1921 ttcatctgca ctaccggaaa gctccctgtt ccgtggccaa ccctcgtcac cactttcacc
     1981 tacggtgttc agtgcttctc ccggtaccca gatcacatga agcagcatga cttcttcaag
     2041 agcgccatgc ccgaaggcta cgtgcaagaa aggactatct tcttcaagga tgacgggaac
     2101 tacaagacac gtgccgaagt caagttcgaa ggtgataccc tggtgaaccg catcgagctg
     2161 aaaggtaagt ttctgcttct acctttgata tatatataat aattatcatt aattagtagt
     2221 aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt
     2281 tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt
     2341 tgttgatgtg caggtatcga tttcaaggaa gatggaaaca tcctcggaca caagctggag
     2401 tacaactaca actcccacaa cgtatacatc atggccgaca agcagaagaa cggcatcaag
     2461 gtgaacttca agatcaggca caacatcgaa gatggaagcg tgcaactggc ggaccactac
     2521 cagcagaaca cgcccatcgg cgatggccct gtcctgctgc cggacaacca ttacctgtcc
     2581 acgcaatctg ccctctccaa ggaccccaac gagaagaggg accacatggt cctgctggag
     2641 ttcgtgacgg ctgctgggat cacgcatggc atggatgaac tctacaagtg acgcgttcga
     2701 gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg
     2761 tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa
     2821 tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt
     2881 gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg
     2941 tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac tgaagcctgc
     3001 aggcatgcaa gctgatccac tagaggccat ggcggccgca ctagtggatc ccccgggatg
     3061 catgaattca agcttatcga tgtcgacagg ccttaagggc cagatcttgg gcccggtacc
     3121 cgatcagatt gtcgtttccc gccttcggtt taaactatca gtgtttgaca ggatatattg
     3181 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     3241 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     3301 gtgcttggca ttccgtgcga taatgacttc tgttcaacca cccaaacgtc ggaaagcctg
     3361 acgacggagc agcattccaa aaagatccct tggctcgtct gggtcggcta gaaggtcgag
     3421 tgggctgctg tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc
     3481 ctcgatcgca aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac
     3541 gtcctggcca gtcatcacgc gccaaagttc cgtcacagga tgatctggcg cgagttgctg
     3601 gatctcgcct tcaatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa
     3661 gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac
     3721 gccgaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3781 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3961 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4501 ccctgattct gtggataacc gattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     4621 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     4681 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     4741 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4801 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4861 caccgtcatc accgaaacgc gcgaggcagg ggtacgtcga ggtcgatcca acccctccgc
     4921 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     4981 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     5041 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     5101 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     5161 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     5221 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     5281 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     5341 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     5401 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     5461 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcgcga
     5521 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     5581 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     5641 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     5701 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     5761 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     5821 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     5881 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctcgcgg
     5941 cctggccggc gagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     6001 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     6061 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     6121 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     6181 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     6241 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     6301 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     6361 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     6421 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     6481 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     6541 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     6601 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     6661 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     6721 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     6781 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     6841 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     6901 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     6961 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     7021 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     7081 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     7141 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     7201 gtggagaagt tgaaggcggc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     7261 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     7321 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     7381 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     7441 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     7501 gggcacgtag aggtttccgc agggccggcc ggcatggcga gtgtgtggga ttacgacctg
     7561 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     7621 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     7681 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     7741 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     7801 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     7861 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     7921 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     7981 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     8041 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     8101 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     8161 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     8221 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     8281 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     8341 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     8401 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     8461 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     8521 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     8581 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     8641 tcgccactcg accgccggcg cccacatcaa ggcaccggtg ggtatgcctg acgatgcgtg
     8701 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg
     8761 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     8821 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     8881 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     8941 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     9001 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     9061 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     9121 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     9181 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     9241 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     9301 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     9361 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     9421 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     9481 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     9541 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     9601 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     9661 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     9721 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     9781 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     9841 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     9901 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     9961 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
    10021 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
    10081 cgcaacatag cttgcttggt cgttccgcgt gaacgtcggc tcgattgtac ctgcgttcaa
    10141 atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg
    10201 cttctctcgc aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt
    10261 gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta
    10321 tctgcctaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag
    10381 cggcagcggc
//
