LOCUS       C007p9o-LH-GFP-o       10557 bp    DNA     circular SYN 29-SEP-2017
DEFINITION  Ligation of B444pmGFPD-ocs* into B415p9o-LH*
ACCESSION   C007p9o-LH-GFP-o
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B415p9o-LH*
               Fragment ends: SfiI
               Fragment size: 9373
            Insert molecule:  B444pmGFPD-ocs*
               Fragment ends: SfiI
               Fragment size: 1184
            Sequencing primers	
            E9F	GTGTCAAATCGTGGCCTCTAA
            SphR	GGGTGGTTGAACAGAAGTCAT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..1043
                     /gene="NptIIo'"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1040..1043
                     /gene="splice"
                     /SECDrawAs="Label"
     CDS             1044..1230
                     /gene="'intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1229..1232
                     /gene="splice"
                     /SECDrawAs="Label"
     CDS             1231..1734
                     /gene="''NptIIo'"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1748..2038
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             2140..2518
                     /gene="GFP"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2519..2707
                     /gene="Int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2708..3045
                     /gene="GFP"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3054..3249
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    3334..3357
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3892..4589
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4626..4885
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5138..8843
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9027..10037
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct
      781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt
      841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg
      901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg
      961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga
     1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat
     1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac
     1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg
     1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact
     1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct
     1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc
     1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc
     1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg
     1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt
     1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg
     1741 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     1801 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     1861 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     1921 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     1981 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga
     2041 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc
     2101 cgggctgcag gaattcaagc ttacgcgtcc tccataacta tgggcaaggg cgaggaactg
     2161 ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg gcacaagttc
     2221 tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct gaagttcatc
     2281 tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt cacctacggt
     2341 gttcagtgct tctcccggta cccagatcac atgaagcagc atgacttctt caagagcgcc
     2401 atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg gaactacaag
     2461 acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga gctgaaaggt
     2521 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
     2581 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
     2641 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     2701 tgtgcaggta tcgatttcaa ggaagatgga aacatcctcg gacacaagct ggagtacaac
     2761 tacaactccc acaacgtata catcatggcc gacaagcaga agaacggcat caaggtgaac
     2821 ttcaagatca ggcacaacat cgaagatgga agcgtgcaac tggcggacca ctaccagcag
     2881 aacacgccca tcggcgatgg ccctgtcctg ctgccggaca accattacct gtccacgcaa
     2941 tctgccctct ccaaggaccc caacgagaag agggaccaca tggtcctgct ggagttcgtg
     3001 acggctgctg ggatcacgca tggcatggat gaactctaca agtgactcgt cgaccctgct
     3061 ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt ctgttgtgca
     3121 cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct
     3181 aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac
     3241 tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccga tcagattgtc
     3301 gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     3361 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     3421 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc
     3481 cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc
     3541 attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg
     3601 cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat
     3661 ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc
     3721 atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca
     3781 atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc
     3841 atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc
     3901 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3961 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4021 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4081 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4141 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4201 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4261 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4321 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4381 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4441 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4501 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4561 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4621 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4681 gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4741 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     4801 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     4861 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     4921 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4981 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5041 gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc tatagtgcag
     5101 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     5161 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     5221 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     5281 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     5341 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     5401 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     5461 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     5521 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     5581 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     5641 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg
     5701 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     5761 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     5821 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     5881 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     5941 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     6001 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     6061 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag
     6121 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     6181 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     6241 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     6301 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     6361 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     6421 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     6481 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     6541 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     6601 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     6661 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     6721 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     6781 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     6841 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     6901 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     6961 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     7021 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     7081 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     7141 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     7201 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     7261 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     7321 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     7381 aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     7441 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     7501 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     7561 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     7621 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     7681 tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg
     7741 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     7801 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     7861 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     7921 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     7981 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     8041 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     8101 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     8161 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     8221 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     8281 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     8341 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     8401 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     8461 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     8521 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     8581 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     8641 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     8701 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     8761 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     8821 gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct
     8881 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
     8941 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     9001 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     9061 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     9121 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     9181 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     9241 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     9301 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     9361 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     9421 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     9481 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     9541 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     9601 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     9661 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     9721 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     9781 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     9841 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     9901 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     9961 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
    10021 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
    10081 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
    10141 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
    10201 atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt
    10261 gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc
    10321 gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac
    10381 gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca
    10441 ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg
    10501 ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggc
//
