LOCUS C074p35s-LBFP 4349 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of LBFP-Bsr-Sal* into B629p35s-LGFP* ACCESSION C074p35s-LBFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4349) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B629p35s-LGFP* Fragment ends: SalI and BsrGI Fragment size: 3586 Insert molecule: LBFP-Bsr-Sal* Fragment ends: BsrGI and SalI Fragment size: 763 Primer for gene synthesis BFPBsr CCGtgtacaggagtaccgccccgtccggtcctgcccGTCACCGAGATCAG BFPSal CCGCGCATCGCACTCGTC Sequencing primers: 35RT CTTCGCAAGACCCTTCCTCT FEATURES Location/Qualifiers misc_feature 106..528 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="35S-Pro" /SECDescr="Promotor-Region" CDS 627..1328 /gene="tagBFP" /SECDrawAs="Gene" /SECStyleId=1 /SECName="tagBFP" misc_feature 884..887 /gene="sp" /SECDrawAs="Label" misc_feature 923..926 /gene="sp" /SECDrawAs="Label" misc_feature 1082..1085 /gene="sp" /SECDrawAs="Label" misc_feature 1124..1127 /gene="sp" /SECDrawAs="Label" misc_feature 1338..1533 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 2349..3209 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 3252..3968 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc 121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccc 541 ccgggctgca ggaattcaag cttacgcgtt gtacaggagt accgccccgt ccggtcctgc 601 ccgtcaccga gatcagcgga gagttcatgt ccgagcttat caaggagaac atgcacatga 661 agctctacat ggagggcacc gtggacaacc accacttcaa gtgcactagc gagggagagg 721 gtaagcccta cgagggcacc cagaccatgc gcatcaaagt cgttgaggga gggccattgc 781 ctttcgcctt cgacattctg gctacatctt tcctctacgg ctccaagacc ttcatcaacc 841 atacgcaagg aatcccggat ttctttaagc agtcattccc cgagggattc acctgggagc 901 gtgtgaccac ctacgaagac ggtggcgtcc tgactgccac ccaggacacc agcctccagg 961 acgggtgctt gatctacaat gtgaagatta ggggcgtcaa cttcacatcc aacggaccag 1021 ttatgcaaaa gaagaccctt ggctgggagg ccttcacgga gaccctgtat cctgccgacg 1081 gaggcctgga gggtcgcaac gatatggcat tgaagctcgt gggcgggtcg cacctgatcg 1141 ctaacgccaa gaccacttac agaagcaaga agcccgcgaa gaacctcaag atgccgggag 1201 tctactacgt ggactaccgt cttgagagga tcaaggaggc caataacgaa acctacgttg 1261 agcagcacga agtcgctgtg gcccgctact gcgacttgcc atccaagctg ggccacaagc 1321 tcaactgacc cggtcgaccc tgctttaatg agatatgcga gacgcctatg atcgcatgat 1381 atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc 1441 ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg tatttttatg 1501 aataatattc tccgttcaat ttactgattg tcctcgacag gccttaaggg ccagatcttg 1561 ggcccggtac ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta 1621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 1681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 1741 cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 1801 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 1861 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 1921 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 1981 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 2041 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 2101 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 2161 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 2221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 2401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 2461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 2521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 2581 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 2641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 2701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 2761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 2821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 2881 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 2941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 3001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 3061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 3301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 3361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 3421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 3481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 3541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 3661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 3721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 3781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 3841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 3901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 3961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 4081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 4141 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 4201 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 4261 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 4321 tgtgtggaat tgtgagcgga taacaattt //