LOCUS       C335p7o-LH-UbiAt       10861 bp    DNA     circular SYN 29-SEP-2017
DEFINITION  Ligation of 204pUbiAtmu-ocs* into B414p7o-LH*
ACCESSION   C335p7o-LH-UbiAt
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B414p7o-LH*
               Fragment ends: SfiI
               Fragment size: 9121
            Insert molecule:  204pUbiAtmu-ocs*
               Fragment ends: SfiI
               Fragment size: 1740
            Sequencing primers	
            BarF	CTTCTGGCAGCTCGACTTCT
            SphR	GGGTGGTTGAACAGAAGTCAT
            Ubq10-F	CTAGATCTGGTGTTAGTTTCTAGT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..978
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    979..1167
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Int"
                     /SECDescr="STLS1"
     CDS             1168..1483
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1496..1786
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1835..3310
                     /gene="Pro-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3005..3308
                     /gene="Int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3358..3553
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    3638..3661
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4196..4893
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4930..5189
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5442..9147
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9331..10341
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt
      781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc
      841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg
      901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc
      961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc
     1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac
     1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc
     1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg
     1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc
     1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt
     1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt
     1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat
     1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt
     1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca
     1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca
     1801 tgcaagctga tccactagag gccatggcgg ccgctttcct tacattctga gcctctttcc
     1861 ttctaatcca ctcatctgca tcttcttgtg tccttactaa tacctcattg gttccaaatt
     1921 ccctcccttt aagcaccagc tcgtttctgt tcttccacag cctcccaagt atccaaggga
     1981 ctaaagcctc cacattcttc agatcaggat attcttgttt aagatgttga actctatgga
     2041 ggtttgtatg aactgatgat ctaggaccgg ataagttccc ttcttcatag cgaacttatt
     2101 caaagaatgt tttgtgtatc attcttgtta cattgttatt aatgaaaaaa tattattggt
     2161 cattggactg aacacgagtg ttaaatatgg accaggcccc aaataagatc cattgatata
     2221 tgaattaaat aacaagaata aatcgagtca ccaaaccact tgcctttttt aacgagactt
     2281 gttcaccaac ttgatacaaa agtcattatc ctatgcaaat caataatcat acaaaaatat
     2341 ccaataacac taaaaaatta aaagaaatgg ataatttcac aatatgttat acgataaaga
     2401 agttactttt ccaagaaatt cactgatttt ataagcccac ttgcattaga taaatggcaa
     2461 aaaaaaacaa aaaggaaaag aaataaagca cgaagaattc tagaaaatac gaaatacgct
     2521 tcaatgcagt gggacccacg gttcaattat tgccaatttt cagctccacc gtatatttaa
     2581 aaaataaaac gataatgcta aaaaaatata aatcgtaacg atcgttaaat ctcaacggct
     2641 ggatcttatg acgaccgtta gaaattgtgg ttgtcgacga gtcagtaata aacggcgtca
     2701 aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc
     2761 ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata
     2821 cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg
     2881 tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac
     2941 aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat
     3001 caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc
     3061 ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc
     3121 tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga
     3181 tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta
     3241 gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg
     3301 attaacaggt actagtggat cccccgggct gcaggaattc aagcttacgc gtgtcgaccc
     3361 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg
     3421 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca
     3481 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat
     3541 ttactgattg tcctcgacag gccttaaggg ccagatcttg ggcccggtac ccgatcagat
     3601 tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     3661 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     3721 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg agtgcttggc
     3781 attccgtgcg ataatgactt ctgttcaacc acccaaacgt cggaaagcct gacgacggag
     3841 cagcattcca aaaagatccc ttggctcgtc tgggtcggct agaaggtcga gtgggctgct
     3901 gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc
     3961 aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc
     4021 agtcatcacg cgccaaagtt ccgtcacagg atgatctggc gcgagttgct ggatctcgcc
     4081 ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg
     4141 gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgaaaag
     4201 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4441 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4981 tgtggataac cgattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5041 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     5101 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5161 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5221 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5281 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5341 caccgaaacg cgcgaggcag gggtacgtcg aggtcgatcc aacccctccg ctgctatagt
     5401 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     5461 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     5521 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     5581 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     5641 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     5701 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     5761 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     5821 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     5881 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     5941 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag
     6001 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     6061 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     6121 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     6181 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     6241 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     6301 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     6361 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctcgcg gcctggccgg
     6421 cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     6481 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     6541 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     6601 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     6661 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     6721 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     6781 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     6841 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     6901 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     6961 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     7021 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     7081 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     7141 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     7201 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     7261 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     7321 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     7381 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     7441 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     7501 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     7561 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     7621 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     7681 ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     7741 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     7801 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     7861 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     7921 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     7981 gaggtttccg cagggccggc cggcatggcg agtgtgtggg attacgacct ggtactgatg
     8041 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     8101 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     8161 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     8221 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     8281 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     8341 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     8401 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     8461 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     8521 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     8581 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     8641 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     8701 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     8761 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     8821 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     8881 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     8941 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     9001 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     9061 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     9121 gaccgccggc gcccacatca aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa
     9181 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt
     9241 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     9301 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     9361 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     9421 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     9481 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     9541 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     9601 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     9661 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     9721 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     9781 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     9841 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     9901 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     9961 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
    10021 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
    10081 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
    10141 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
    10201 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
    10261 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
    10321 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
    10381 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
    10441 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
    10501 tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata
    10561 gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta cctgcgttca aatactttgc
    10621 gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca cggatcgact gcttctctcg
    10681 caacgccatc cgacggatga tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg
    10741 ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc aatggaaatt atctgcctaa
    10801 ccggctcagt tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg
    10861 c
//
