LOCUS C384pRedUH-35s-o 9690 bp DNA circular SYN 29-SEP-2017 DEFINITION Ligation of UbiH-Mfe-Xho* into B469pRedU10-35s-ocs-LH* ACCESSION C384pRedUH-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9690) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B469pRedU10-35s-ocs-LH* Fragment ends: XhoI and MfeI Fragment size: 9051 Insert molecule: UbiH-Mfe-Xho* Fragment ends: MfeI and XhoI Fragment size: 639 Primer for gene synthesis LB CTCAGTTCTGCGTAGAAACCA nptoR CACTGCACCCAATGGTCTGTT Sequencing primers LB CTCAGTTCTGCGTAGAAACCA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 316..651 /gene="Ubi" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 671..1366 /gene="dsRed" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1383..1663 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1718..2140 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="35S-Pro" /SECDescr="Promotor-Region" misc_feature 2187..2382 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 2467..2490 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3025..3722 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3759..4018 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4271..7976 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8160..9170 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacc gcgacaaaac ttaagaaagt caccaaaaag aaagtcaaag acggcaacgg 361 caacccagaa gctacgttct ctttctagat tctatgccct acctaccgcg tggtgcacaa 421 caacttgacc cccatcattg actctccacc gtacacgtgt cacgatacca tttgttggtc 481 tcctaccact tacgtaacat taatattcca tccgtgaccg cctcttcttc atcatcctat 541 aaattcacct tcttatctct tccgctttca ttcgttcatc atcatcacaa ttaacaacaa 601 tccaaagcaa tcttctctca tattcttatc tcttcctgtt tcgcttcgtc tgctcgagtc 661 ctccataaca atgcagatct ttgccatggc gcgctcctcc aagaacgtca tcaaggagtt 721 catgcgcttc aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg 781 cgagggcgag ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg 841 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt 901 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt 961 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 1021 ctcccttcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc 1081 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta 1141 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg 1201 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg 1261 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt 1321 ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgtagtccg gagtattatg 1381 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt 1441 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat 1501 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg 1561 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat 1621 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac actgaagcct gcaggcatgc 1681 aagctgatcc actagaggcc atggcggccg cactagatca acatggtgga gcacgacact 1741 ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc tattgagact 1801 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 1861 ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa 1921 ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc 1981 acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga 2041 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 2101 tcctctatat aaggaagttc atttcatttg gagaggacac tagttggatc ccccgggctg 2161 caggaattca agcttacgcg tgtcgaccct gctttaatga gatatgcgag acgcctatga 2221 tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag 2281 ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt 2341 atttttatga ataatattct ccgttcaatt tactgattgt cctcgacagg ccttaagggc 2401 cagatcttgg gcccggtacc cgatcagatt gtcgtttccc gccttcggtt taaactatca 2461 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat 2521 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 2581 ccacagggtt cccctcggga gtgcttggca ttccgtgcga taatgacttc tgttcaacca 2641 cccaaacgtc ggaaagcctg acgacggagc agcattccaa aaagatccct tggctcgtct 2701 gggtcggcta gaaggtcgag tgggctgctg tggcttgatc cctcaacgcg gtcgcggacg 2761 tagcgcagcg ccgaaaaatc ctcgatcgca aatccgacgc tgtcgaaaag cgtgatctgc 2821 ttgtcgctct ttcggccgac gtcctggcca gtcatcacgc gccaaagttc cgtcacagga 2881 tgatctggcg cgagttgctg gatctcgcct tcaatccggg tctgtggcgg gaactccacg 2941 aaaatatccg aacgcagcaa gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg 3001 ccgacgccgt tgatgtggac gccgaaaagg atctaggtga agatcctttt tgataatctc 3061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 3121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 3181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 3241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 3301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 3361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 3421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 3481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 3541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 3601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 3661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 3721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 3781 atgttctttc ctgcgttatc ccctgattct gtggataacc gattaccgcc tttgagtgag 3841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 3901 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 3961 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 4021 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 4081 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 4141 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg ggtacgtcga 4201 ggtcgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt 4261 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct 4321 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga 4381 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg 4441 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca 4501 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga 4561 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc 4621 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc 4681 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg 4741 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc 4801 gcgaggccgc caaggcgcga ggcgtgaagt ttggcccccg ccctaccctc accccggcac 4861 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg 4921 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga 4981 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg 5041 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg 5101 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta 5161 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt 5221 gtctgatgcc aagctcgcgg cctggccggc gagcttggcc gctgaagaaa ccgagcgccg 5281 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta 5341 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta 5401 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg 5461 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat 5521 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt 5581 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag 5641 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag 5701 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt 5761 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg 5821 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc 5881 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa 5941 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca 6001 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg 6061 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc 6121 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt 6181 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac 6241 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc 6301 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg 6361 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc 6421 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg 6481 cggcgctggg tgatgacctg gtggagaagt tgaaggcggc gcaggccgcc cagcggcaac 6541 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca 6601 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg 6661 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca 6721 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga 6781 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcga 6841 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc 6901 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg 6961 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct 7021 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc 7081 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg 7141 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca 7201 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg 7261 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat 7321 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt 7381 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg 7441 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat 7501 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag 7561 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg 7621 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt 7681 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta 7741 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag 7801 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc 7861 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag 7921 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccggtg 7981 ggtatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa 8041 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga 8101 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta 8161 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg 8221 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca 8281 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta 8341 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg 8401 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt 8461 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata 8521 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca 8581 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag 8641 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc 8701 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg 8761 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc 8821 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg 8881 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc 8941 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg 9001 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta 9061 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag 9121 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa 9181 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc 9241 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc 9301 ataataagcc ctacacaaat tgggagatat atcatgaaag gctggctttt tcttgttatc 9361 gcaatagttg gcgaagtaat cgcaacatag cttgcttggt cgttccgcgt gaacgtcggc 9421 tcgattgtac ctgcgttcaa atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg 9481 ctgatctcac ggatcgactg cttctctcgc aacgccatcc gacggatgat gtttaaaagt 9541 cccatgtgga tcactccgtt gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc 9601 tcagttctca atggaaatta tctgcctaac cggctcagtt ctgcgtagaa accaacatgc 9661 aagctccacc gggtgcaaag cggcagcggc //