LOCUS       C387pGusXM              4984 bp    DNA     circular SYN 04-OCT-2017
DEFINITION  Ligation of GusM-Hind-Sal* into pOCS-BM*
ACCESSION   C387pGusXM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pOCS-BM*
               Fragment ends: SalI and HindIII
               Fragment size: 3146
            Insert molecule:  GusM-Hind-Sal*
               Fragment ends: HindIII and SalI
               Fragment size: 1838
            Sequencing primers:
            M13RY	CACACAGGAAACAGCTATGAC     gc rich
            OR2	GTGCACAACAGAATTGAAAGCAAA     gc rich
FEATURES             Location/Qualifiers
     CDS             182..1993
                     /gene="GusX"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1999..2194
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    3010..3870
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3913..4629
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggctgcagg aattcaagct tggcacgcgt cctccaccga
      181 aatgctccgc cccgtggaga ccccaactcg tgagatcaag aagctggacg gcttgtgggc
      241 cttctccctt gacagggaaa actgcggaat tgatcagaga tggtgggagt ccgctctcca
      301 agagtctcgc gccatcgccg tccctggttc gttcaacgac cagttcgcag acgctgacat
      361 ccgcaattac gccggcaacg tttggtatca gagggaagtc ttcatcccga aggggtgggc
      421 cggacagcgc attgtcctgc gctttgatgc ggtgacccac tacggaaaag tctgggttaa
      481 caaccaagaa gtgatggagc accagggcgg atacacaccc ttcgaagccg acgtcacccc
      541 atacgtgatc gccggcaagt ccgtccgtat caccgtttgc gtgaacaacg agttgaattg
      601 gcagaccatc ccaccaggca tggtgattac ggacgagaac ggtaagaaga agcaaagcta
      661 tttccacgac ttcttcaact acgccggaat ccacaggagc gtgatgctct acaccactcc
      721 taacacctgg gtggacgata tcaccgtcgt tacccatgtc gcccaggact gcaaccacgc
      781 ctccgttgac tggcaggtgg tggccaatgg cgacgtttca gtcgagctta gagatgccga
      841 ccagcaagtg gtcgcaactg gacagggaac ctccggcacc ctccaagtgg tcaacccgca
      901 cctgtggcag cccggtgagg gctacctcta cgagttgtgc gttacagcta agagtcagac
      961 cgagtgcgac atttacccac tgcgcgtggg aatccgttct gtcgccgtga agggcgagca
     1021 gttcctcatc aaccacaagc ccttctactt cacggggttt ggccgccacg aggacgccga
     1081 tcttagggga aagggcttcg acaacgtctt gatggttcac gaccacgctc tcatggactg
     1141 gatcggtgcc aactcctacc gcaccagcca ctacccttac gccgaggaga tgctggactg
     1201 ggcggatgaa cacggaattg tggtcatcga cgagaccgct gctgtgggct tcaacctctc
     1261 gcttgggatc ggattcgagg ccggaaataa gcccaaggag ctgtactccg aggaggccgt
     1321 caacggcgaa actcagcagg ctcacctcca ggccattaag gagttgatcg caagagacaa
     1381 gaaccatcca agcgtggtta tgtggtccat cgccaacgag ccggacaccc gtccccaggg
     1441 agctagggag tacttcgccc cactcgccga ggctacccgc aagctcgatc ccacccgccc
     1501 tatcacttgc gtcaacgtga tgttctgcga cgcccacacc gacacaattt cagacctttt
     1561 cgacgtcttg tgcctcaacc gttattacgg ctggtacgtg cagagcggtg atctggagac
     1621 cgccgaaaag gttttggaga aggagctgct cgcgtggcaa gagaagctcc accagccgat
     1681 catcatcacc gagtacgggg tggacacgct ggccggactc cactccatgt ataccgatat
     1741 gtggtctgag gaataccagt gcgcttggct tgacatgtac cacagggtgt tcgaccgtgt
     1801 gtcggccgtg gttggagagc aagtctggaa ttttgcagac ttcgccacca gccagggcat
     1861 tttgagagtg ggaggcaaca agaaaggtat cttcactcgt gacaggaagc caaagtccgc
     1921 tgccttcctg ctccagaagc gctggaccgg catgaacttc ggggagaagc cccagcaagg
     1981 aggcaagcag taagtcgacc ctgctttaat gagatatgcg agacgcctat gatcgcatga
     2041 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
     2101 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
     2161 gaataatatt ctccgttcaa tttactgatt gtcctcgaca ggccttaagg gccagatctt
     2221 gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     2281 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2341 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2401 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     2461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2521 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     2581 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     2641 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     2701 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     2761 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     2821 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     2881 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2941 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3001 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3061 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3121 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3181 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3241 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3301 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3361 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3421 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3481 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3541 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3601 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3661 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3721 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3781 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3841 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3901 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3961 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4741 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4801 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4861 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4921 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     4981 ttgt
//
