DCSPol is a highly processive, thermostable DNA polymerase. Due to its genetic modifications DCSPol has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.
| Cat. No. | Pack Size | Conc. |
| DPT-500 | 500 U | 5 U/µl |
For in vitro use only
50,00 € excl. VAT
Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
5 U/µl
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into an acid-insoluble form in 30 minutes at 74ºC.
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of DCSPol DNA Polymerase.
The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. SDS/PAGE - 95 kD band, >98% pure. Activity and stability tested via thermo-cycling. The error rate per nucleotide per cycle is ~ 2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life at 95ºC is 1.5 hours.
| Component | Volume | Final conc. |
| DCSPol (5 U/µl) | 0.4-1.0 µl | 0.02-0.05 U/µl (2-5 U) |
| 10 x Buffer B or BD | 10 µl | 1x |
| 25 mM MgCl2 | 6-10 µl | 1.5-2.5 mM |
| 20 mM dNTP mix | 1 µl | 200 µM |
| Primer Forward (10 pmol/µl) | 1-3 µl | 0.1-0.3 µM |
| Primer Reverse (10 pmol/µl) | 1-3 µl | 0.1-0.3 µM |
| DNA template | 5-20 µl | 5-100 ng/µl |
| 10 x Solution S Not for standard PCR | 0, 10, 20 or 30 µl | 1x, 2x or 3x |
| H2O PCR grade | Up to 100 µl | |
| Total | 100 µl |
| Cycle step | Temp. | Time | Cycles |
| Initial denaturation | 95ºC | 3-5 min | 1 |
| Denaturation | 95ºC | 30-60 s | 26-35 |
| Annealing | 50-68ºC | 30-60 s | |
| Elongation | 72ºC | 1-4 min | |
| Final elongation | 72ºC | 5-10 min | 1 |
IMPORTANT: Annealing temperature should be 2-6ºC lower than the primer melting temperature. Elongation time should be ~1 min/1 kb.
This product and its components should be handled only by persons trained in laboratory techniques. It is advisable to wear suitable protective clothing, such as laboratory overalls, gloves and safety glasses. Care should be taken to avoid contact with skin or eyes. In case of contact with skin or eyes, wash immediately with water.
Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.
