LOCUS       B480p9oUZm-LH          10537 bp    DNA     circular SYN 26-JAN-2016
DEFINITION  Ligation of UbiZm-Bsa* into B475p9oM-LH*
ACCESSION   B480p9oUZm-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B475p9oM-LH*
               Fragment ends: XhoI and XbaI
               Fragment size: 9020
            Insert molecule:  UbiZm-Bsa*
               Fragment ends: BsaI
               Fragment size: 1517
            Primer for gene synthesis
            BsaXba	ccGGTCTCtctagatCTGCAGTGCAGCGT
            BsaXho	ccGGTCTCctcgagGACCTGCAGAAGTAACAC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
            UF1	CGAGTAGATAATGCCAGCC
FEATURES             Location/Qualifiers
     CDS             62..1569
                     /gene="UbiZm"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="UbiZm"
                     /SECDescr="Promotor"
     misc_feature    1049..1568
                     /gene="int"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron"
     CDS             1590..1888
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1889..2075
                     /gene="int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2076..2582
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2600..2890
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3066..3089
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3624..4321
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4870..8575
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8759..9769
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    10290..10313
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga
       61 tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
      541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
      601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
      661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
      721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
      781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
      841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
      901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
      961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
     1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga
     1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     1561 ttctgcaggt cctcgagtcc tccaccgaaa tgggagcatg gattgagcag gatggcctcc
     1621 atgctggtag ccccgctgct tgggtggagc gcctctttgg ctacgactgg gcccaacaga
     1681 ccattgggtg cagtgatgcc gctgtcttcc gtttgagcgc ccagggcagg ccagttctct
     1741 tcgtgaagac tgacctctcc ggtgccctca acgagttgca ggacgaagct gcccgcctct
     1801 catggcttgc caccaccgga gtcccttgcg ctgccgtgtt ggacgttgtc acagaggccg
     1861 gaagagactg gctgctcttg ggcgaggtaa gtttctgctt ctacctttga tatatatata
     1921 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     1981 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     2041 tttctaatat atgaccaaaa tttgttgatg tgcaggtgcc aggccaagac ctcctgagct
     2101 cccacctggc cccagccgag aaggtctcca tcatggcaga cgccatgcgt cgcctccaca
     2161 ccctggaccc agccacctgc cccttcgacc accaggccaa gcacaggatc gaacgtgcac
     2221 gcactaggat ggaggccggc cttgtggacc aggatgacct tgacgaggag caccagggcc
     2281 ttgctcccgc cgagctgttc gcccgcctca aggctagaat gcctgacgga gaggacttgg
     2341 tggtcaccca cggggatgcc tgcctcccca acatcatggt tgagaacgga cgcttctctg
     2401 gcttcatcga ctgcggtcgt ctgggcgtgg ccgaccgcta ccaagacatc gccctggcca
     2461 ccagggacat cgctgaggaa cttggagggg agtgggccga tcgtttcttg gtcctctacg
     2521 gcattgctgc ccccgactcc cagaggatcg ccttctaccg cctgcttgac gagttcttct
     2581 gatccggaat cgcgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
     2641 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
     2701 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
     2761 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
     2821 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
     2881 gtaaaacact gaagcctgca ggcatgcaag ctgatccact agaggccatg gcggccgcac
     2941 tagtggatcc cccgggatgc atgaattcaa gcttatcgat gtcgacaggc cttaagggcc
     3001 agatcttggg cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag
     3061 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     3121 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     3181 cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac
     3241 ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg
     3301 ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt
     3361 agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct
     3421 tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat
     3481 gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga
     3541 aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc
     3601 cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca
     3661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4381 tgttctttcc tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc
     4441 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4501 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     4561 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     4621 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     4681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag
     4801 gtcgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
     4861 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
     4921 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
     4981 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
     5041 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
     5101 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
     5161 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
     5221 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
     5281 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
     5341 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
     5401 cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
     5461 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
     5521 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
     5581 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
     5641 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
     5701 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
     5761 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
     5821 tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac cgagcgccgc
     5881 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     5941 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     6001 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     6061 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     6121 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     6181 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     6241 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     6301 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     6361 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     6421 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     6481 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     6541 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     6601 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     6661 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     6721 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     6781 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     6841 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     6901 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     6961 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     7021 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     7081 ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc agcggcaacg
     7141 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     7201 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     7261 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     7321 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     7381 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggcgag
     7441 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     7501 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     7561 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     7621 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     7681 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     7741 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     7801 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     7861 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     7921 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     7981 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     8041 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     8101 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     8161 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     8221 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     8281 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     8341 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     8401 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     8461 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     8521 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccggtgg
     8581 gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     8641 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     8701 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     8761 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     8821 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     8881 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     8941 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     9001 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     9061 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     9121 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     9181 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     9241 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     9301 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     9361 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     9421 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     9481 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     9541 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     9601 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     9661 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     9721 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat
     9781 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     9841 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     9901 taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg
     9961 caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct
    10021 cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc
    10081 tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg tttaaaagtc
    10141 ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct
    10201 cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca
    10261 agctccaccg ggtgcaaagc ggcagcggcg gcaggatata ttcaattgta aatggcttca
    10321 tgtccgggaa atctacatgg atcagcaatg agtatgatgg tcaatatgga gaaaaagaaa
    10381 gagtaattac caattttttt tcaattcaaa aatgtagatg tccgcagcgt tattataaaa
    10441 tgaaagtaca ttttgataaa acgacaaatt acgatccgtc gtatttatag gcgaaagcaa
    10501 taaacaaatt attctaattc ggaaatcttt atttcga
//
