LOCUS p6D35s-2xU10 10357 bp DNA circular SYN 17-SEP-2025 DEFINITION p6D35s-2xU10 (G948): Ligation of U10-Xma-Bsr* into F918p6D35s-2xU10* ACCESSION p6D35s-2xU10 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p6D35s-2xU10 FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 169..584 /label="35S" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 600..604 /label="Kozak" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 605..1174 /label="hptII" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1175..1363 /label="int" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 1364..1819 /label="'hptII''" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1830..2120 /label="'E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2146..3621 /label="Pro-Ubq10At" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 3250..3313 /label="Ubi cds 5`" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 3316..3619 /label="íntron" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 3649..3902 /label="NOS-T" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 3923..5398 /label="Pro-Ubq10At" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 5027..5090 /label="Ubi cds 5`" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 5093..5396 /label="íntron" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 5435..5630 /label="OCS" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 5675..6277 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 5720..5743 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 6291..6949 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 6978..8941 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 7304..8479 /label="ParB" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 9130..10140 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(10208..10357,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc 181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat 601 aactatgaag aaaccggagc tgaccgccac ttccgtcgaa aagttcctca tcgagaagtt 661 tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa gagtcccgcg cgttcagctt 721 tgatgttggc gggcgcgggt acgtcctccg cgtgaactct tgcgctgacg gcttctataa 781 agatcggtac gtttacaggc atttcgcctc ggcggccctg cctattcccg aagtcctcga 841 catcggcgag ttttccgaga gccttacgta ttgtatttcc cgccgggctc aaggggtgac 901 cctccaggat ctgccggaaa ctgagttgcc tgcggttctc cagcccgtcg ccgaggctat 961 ggacgccatc gccgcggcgg atctttctca aacgtcgggc ttcgggccgt tcggccctca 1021 ggggattggc cagtacacca cttggaggga ctttatctgc gctatcgccg atccgcacgt 1081 gtatcattgg caaaccgtta tggacgatac ggtgtccgcg agcgtggccc aggctctgga 1141 cgaactcatg ttgtgggccg aggattgccc cgaggtaagt ttctgcttct acctttgata 1201 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa 1261 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat 1321 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggtccgcc acctggttca 1381 tgcggacttc ggctctaata acgtgctcac cgataatggg cggattactg ccgtcatcga 1441 ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg gcgaacattt tcttctggag 1501 gccttggctt gcctgcatgg aacagcaaac gcgctacttt gagcggaggc acccggagct 1561 ggcggggtca ccccgcctca gggcctatat gttgcggatc ggcctcgacc agctttacca 1621 gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg caaggccgct gtgacgctat 1681 tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc gcccggcggt cggctgccgt 1741 ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc aacaggcggc cttccacccg 1801 cccgcgggcc aaggagtgaa cgcgttcgag tattatggca ttgggaaaac tgtttttctt 1861 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg 1921 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa 1981 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat 2041 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt 2101 aatatgagga gtaaaacact gaagcctgca ggccttggcg gccgctttcc ttacattctg 2161 agcctctttc cttctaatcc actcatctgc atcttcttgt gtccttacta atacctcatt 2221 ggttccaaat tccctccctt taagcaccag ctcgtttctg ttcttccaca gcctcccaag 2281 tatccaaggg actaaagcct ccacattctt cagatcagga tattcttgtt taagatgttg 2341 aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc cttcttcata 2401 gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat taatgaaaaa 2461 atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc caaataagat 2521 ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac ttgccttttt 2581 taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa tcaataatca 2641 tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca caatatgtta 2701 tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca cttgcattag 2761 ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt ctagaaaata 2821 cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt tcagctccac 2881 cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa 2941 tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat 3001 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac 3061 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac 3121 aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg 3181 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc 3241 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc 3301 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt 3361 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga 3421 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag 3481 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg 3541 atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct 3601 gagtttttct gattaacagg tactagtctc ggatccctct ccggacccga tcgttcaaac 3661 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata 3721 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt 3781 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac 3841 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat 3901 cgctcgaccc gggagacgcc gctttcctta cattctgagc ctctttcctt ctaatccact 3961 catctgcatc ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa 4021 gcaccagctc gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca 4081 cattcttcag atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa 4141 ctgatgatct aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt 4201 tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa 4261 cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa 4321 caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt 4381 gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta 4441 aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc 4501 aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa 4561 aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg 4621 gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga 4681 taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac 4741 gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag 4801 ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa 4861 ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt 4921 tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt 4981 cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc 5041 aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt 5101 ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat 5161 atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg 5221 ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata 5281 atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta 5341 gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacaggtac 5401 tgctgtacag acaagcttca cgctagcgtg tcgaccctgc tttaatgaga tatgcgagac 5461 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc 5521 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt 5581 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtcc tcgacaggcc 5641 ttaacggcca gatctgctgg gccctgtggt acccgatcag attgtcgttt cccgccttcg 5701 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 5761 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt 5821 atgtgcatgc caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac 5881 ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc 5941 ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac 6001 gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa 6061 aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag 6121 ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg 6181 cgggaactcc acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc 6241 tgggcagtcg ccgccgacgc cgttgatgtg gacgccggct tagctcatgg ccaaaatccc 6301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 6361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac catcgctacc 6421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 6481 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 6541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 6601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 6661 ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac 6721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 6781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 6841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 6901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 6961 cgcggccttt cctaagggtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct 7021 aagtgccggt cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga 7081 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa 7141 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca 7201 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag 7261 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag 7321 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg 7381 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat 7441 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg 7501 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagccgccg ctgatcgaat 7561 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa 7621 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag 7681 tcgcagcatc atggacgtgg cagtattccg cctctcgaaa cgtgatcgcc gggcgggcga 7741 agtcatccgc tacgaactgc cggacggtca tgtcgaggtg agcgcaggcc cggcgggcat 7801 ggcctccgtc tgggattatg acctcgttct gatggccgtg tcgcacctta cggaaagcat 7861 gaatcgctac cgggagggca agggtgataa gcccggtcgc gtattccgtc cgcatgtggc 7921 cgacgttctg aaattctgtc gccgggcgga tggcggcaag caaaaggacg atctcgtcga 7981 aacctgcatc cgcctgaaca cgacccacgt cgccatgcag cgcaccaaga aagcgaagaa 8041 tggccgtctt gtgactgtct ccgagggcga agccctgatc tcgcgctaca agatcgtaaa 8101 gagcgaaacc gggcggcccg agtacatcga gatcgagctt gctgattgga tgtaccgcga 8161 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgattga 8221 tcccggcatc ggtcgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc 8281 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt 8341 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga 8401 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga 8461 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga 8521 aaaaggtcga aaagctctct ttcctgtgga tagcacgtac attgggaacc caaagccgta 8581 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accgggcacg 8641 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa 8701 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc 8761 cgaatagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc 8821 gcgtcggcct atcgtggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct 8881 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cgccgcccac atcaaggcac 8941 ctcagcggtg ggtatgcctg acgatgcgtg cagaccgaaa ccttgcgctc gttcgccagc 9001 caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg 9061 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc 9121 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac 9181 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct 9241 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc 9301 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gcgtgaagcg 9361 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc 9421 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca 9481 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga 9541 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc 9601 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag 9661 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca 9721 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc 9781 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag 9841 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga 9901 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg 9961 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata 10021 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc tcgcgcagat 10081 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa 10141 tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga 10201 cggaccgccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc 10261 acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc 10321 aacatgcaag ctccaccggg tgcaaagcgg cagcggc //