LOCUS p6D35s-GusiX2 9120 bp DNA circular SYN 08-MAR-2024 DEFINITION p6D35s-GusiX2 (G103): Ligation of Gus-Bam-Bst* into F475p6D35s-GusiX* ACCESSION p6D35s-GusiX2 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p6D35s-GusiX2 FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 169..584 /label="35S" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 600..604 /label="Kozak" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 605..1174 /label="hptII" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1175..1363 /label="int" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 1364..1819 /label="'hptII''" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1830..2120 /label="'E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" CDS 2194..2808 /label="'GusX'''" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 2809..2997 /label="''Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 2998..4194 /label="''GusX" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 4200..4395 /label="OCS" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 4438..5040 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 4483..4506 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 5054..5712 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 5741..7704 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 6067..7242 /label="ParB" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 7893..8903 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(8971..9120,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc 181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat 601 aactatgaag aaaccggagc tgaccgccac ttccgtcgaa aagttcctca tcgagaagtt 661 tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa gagtcccgcg cgttcagctt 721 tgatgttggc gggcgcgggt acgtcctccg cgtgaactct tgcgctgacg gcttctataa 781 agatcggtac gtttacaggc atttcgcctc ggcggccctg cctattcccg aagtcctcga 841 catcggcgag ttttccgaga gccttacgta ttgtatttcc cgccgggctc aaggggtgac 901 cctccaggat ctgccggaaa ctgagttgcc tgcggttctc cagcccgtcg ccgaggctat 961 ggacgccatc gccgcggcgg atctttctca aacgtcgggc ttcgggccgt tcggccctca 1021 ggggattggc cagtacacca cttggaggga ctttatctgc gctatcgccg atccgcacgt 1081 gtatcattgg caaaccgtta tggacgatac ggtgtccgcg agcgtggccc aggctctgga 1141 cgaactcatg ttgtgggccg aggattgccc cgaggtaagt ttctgcttct acctttgata 1201 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa 1261 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat 1321 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggtccgcc acctggttca 1381 tgcggacttc ggctctaata acgtgctcac cgataatggg cggattactg ccgtcatcga 1441 ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg gcgaacattt tcttctggag 1501 gccttggctt gcctgcatgg aacagcaaac gcgctacttt gagcggaggc acccggagct 1561 ggcggggtca ccccgcctca gggcctatat gttgcggatc ggcctcgacc agctttacca 1621 gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg caaggccgct gtgacgctat 1681 tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc gcccggcggt cggctgccgt 1741 ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc aacaggcggc cttccacccg 1801 cccgcgggcc aaggagtgaa cgcgttcgag tattatggca ttgggaaaac tgtttttctt 1861 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg 1921 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa 1981 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat 2041 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt 2101 aatatgagga gtaaaacact gaagcctgca ggccttggcg gccgcagcac tagtcgtgga 2161 tcctcatgta caggtgaatt ctcctccata actatgctca gaccggtgga gaccccaacc 2221 cgggagatca agaagcttga cggcttgtgg gccttctccc ttgacaggga aaactgcgga 2281 attgatcaga gatggtggga gtccgctctc caagagtctc gcgccatcgc cgtccctggt 2341 tcgttcaacg accagttcgc agacgctgac atccgcaatt acgccggcaa cgtttggtat 2401 cagagggaag tcttcatccc gaaggggtgg gccggacagc gcattgtcct gcgctttgat 2461 gcggtgaccc actacggaaa agtctgggtt aacaaccaag aagtgatgga gcaccagggc 2521 ggatacacac ccttcgaagc cgacgtcacc ccatacgtga tcgccggcaa gtccgtccgt 2581 atcaccgttt gcgtgaacaa cgagttgaat tggcagacca tcccaccagg catggtgatt 2641 acggacgaga acggtaagaa gaagcaaagc tatttccacg acttcttcaa ctacgccgga 2701 atccacagga gcgtgatgct ctacaccact cctaacacct gggtggacga tatcaccgtc 2761 gttacccatg tcgcccagga ctgcaaccac gcctccgttg actggcaggt aagtttctgc 2821 ttctaccttt gatatatata taataattat cattaattag tagtaatata atatttcaaa 2881 tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt 2941 gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggtg 3001 gtggccaatg gcgacgtttc agtcgagctt agagatgccg accagcaagt ggtcgcaact 3061 ggacagggaa cctccggcac cctccaagtg gtcaacccgc acctgtggca gcccggtgag 3121 ggctacctct acgagttgtg cgttacagct aagagtcaga ccgagtgcga catttaccca 3181 ctgcgcgtgg gaatccgttc tgtcgccgtg aagggcgagc agttcctcat caaccacaag 3241 cccttctact tcacggggtt tggccgccac gaggacgccg atcttagggg aaagggcttc 3301 gacaacgtct tgatggttca cgaccacgct ctcatggact ggatcggtgc caactcctac 3361 cgcaccagcc actaccctta cgccgaggag atgctggact gggcggatga acacggaatt 3421 gtggtcatcg acgagaccgc tgctgtgggc ttcaacctct cgcttgggat cggattcgag 3481 gccggaaata agcccaagga gctgtactcc gaggaggccg tcaacggcga aactcagcag 3541 gctcacctcc aggccattaa ggagttgatc gcaagagaca agaaccatcc aagcgtggtt 3601 atgtggtcca tcgccaacga gccggacacc cgtccccagg gagctaggga gtacttcgcc 3661 ccactcgccg aggctacccg caagctcgat cccacccgcc ctatcacttg cgtcaacgtg 3721 atgttctgcg acgcccacac cgacacaatt tcagaccttt tcgacgtctt gtgcctcaac 3781 cgttattacg gctggtacgt gcagagcggt gatctggaga ccgccgaaaa ggttttggag 3841 aaggagctgc tcgcgtggca agagaagctc caccagccga tcatcatcac cgagtacggg 3901 gtggacacgc tggccggact ccactccatg tataccgata tgtggtctga ggaataccag 3961 tgcgcttggc ttgacatgta ccacagggtg ttcgaccgtg tgtcggccgt ggttggagag 4021 caagtctgga attttgcaga cttcgccacc agccagggca ttttgagagt gggaggcaac 4081 aagaaaggta tcttcactcg tgacaggaag ccaaagtccg ctgccttcct gctccagaag 4141 cgctggaccg gcatgaactt cggggagaag ccccagcaag gaggcaagca gtaagtcgac 4201 cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt 4261 tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt 4321 cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca 4381 atttactgat tgtcctcgac aggccttaag ggccagatct tgggccctgt ggtacccgat 4441 cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg 4501 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa 4561 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc 4621 ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga 4681 cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg 4741 ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg 4801 atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc 4861 tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc 4921 tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata 4981 tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg 5041 gcttagctca tggccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 5101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 5161 caaacaaaaa aaccatcgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 5281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 5341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 5401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgctca 5461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5701 agcctatgga aaaacgccag caacgcggcc tttcctaagg gttaatgagg taaagagaaa 5761 atgagcaaaa gcacaaacac gctaagtgcc ggtcgtccga gcgcacgcag cagcaaggct 5821 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 5881 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 5941 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 6001 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 6061 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt 6121 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc 6181 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg 6241 aaggcggcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg 6301 tggcaagccg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg 6361 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc 6421 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggcagtatt ccgcctctcg 6481 aaacgtgatc gccgggcggg cgaagtcatc cgctacgaac tgccggacgg tcatgtcgag 6541 gtgagcgcag gcccggcggg catggcctcc gtctgggatt atgacctcgt tctgatggcc 6601 gtgtcgcacc ttacggaaag catgaatcgc taccgggagg gcaagggtga taagcccggt 6661 cgcgtattcc gtccgcatgt ggccgacgtt ctgaaattct gtcgccgggc ggatggcggc 6721 aagcaaaagg acgatctcgt cgaaacctgc atccgcctga acacgaccca cgtcgccatg 6781 cagcgcacca agaaagcgaa gaatggccgt cttgtgactg tctccgaggg cgaagccctg 6841 atctcgcgct acaagatcgt aaagagcgaa accgggcggc ccgagtacat cgagatcgag 6901 cttgctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt 6961 caccccgatt actttttgat tgatcccggc atcggtcgtt ttctctaccg cctggcacgc 7021 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc 7081 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac 7141 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc 7201 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta 7261 gggcaaattg ccctagcagg ggaaaaaggt cgaaaagctc tctttcctgt ggatagcacg 7321 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag 7381 ccgtacattg ggaaccgggc acgcatgtaa gtgactgata taaaagagaa aaaaggcgat 7441 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca 7501 taactgtctg gccagcgcac agccgaatag ctgcaaaaag cgcctaccct tcggtcgctg 7561 cgctccctac gccccgccgc ttcgcgtcgg cctatcgtgg ccgctggccg ctcaaaaatg 7621 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac 7681 cgccgccgcc cacatcaagg cacctcagcg gtgggtatgc ctgacgatgc gtgcagaccg 7741 aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg 7801 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct 7861 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 7921 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 7981 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 8041 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 8101 agttaaacat catgcgtgaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 8161 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 8221 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 8281 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 8341 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 8401 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 8461 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 8521 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 8581 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 8641 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 8701 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 8761 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 8821 atcgcttggc ctctcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 8881 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg 8941 cgcggcttaa ctcaagcgtt agacggaccg cccatgtgga tcactccgtt gccccgtcgc 9001 tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta tctgcctaac 9061 cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc //