LOCUS C242p6M2x35s-UbiZmGFP 9915 bp DNA circular SYN 10-APR-2017 DEFINITION Ligation of B595pUbiZm-mGFPM-ocs* into C241p6M2x35s* ACCESSION C242p6M2x35s-UbiZmGFP KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /SECDrawAs="Gene" misc_feature 174..958 /label="P2x35s'" /SECDrawAs="Region" misc_feature 971..975 /label="Kozak" /SECDrawAs="Region" CDS 976..1545 /label="hptII" /SECDrawAs="Gene" misc_feature 1546..1734 /label="int" /SECDrawAs="Gene" CDS 1735..2190 /label="'hptII" /SECDrawAs="Gene" misc_feature 2208..2498 /label="'E9" /SECDrawAs="Region" misc_feature 2546..4053 /label="'Ubi-int" /SECDrawAs="Region" misc_feature 3532..4051 /label="Intron" /SECDrawAs="Region" CDS 4079..4457 /label="GFP" /SECDrawAs="Gene" misc_feature 4458..4646 /label="ST-LS1" /SECDrawAs="Region" CDS 4647..4984 /label="GFP" /SECDrawAs="Gene" misc_feature 4993..5188 /label="OCS" /SECDrawAs="Region" misc_feature 5233..5835 /label="Right Border Region" /SECDrawAs="Region" misc_feature 5278..5301 /label="RB" /SECDrawAs="Gene" misc_feature 5849..6507 /label="'ColE1''" /SECDrawAs="Region" misc_feature 6536..8499 /label="''pVS1 ORI'''" /SECDrawAs="Region" CDS 8688..9698 /label="strep/spec" /SECDrawAs="Gene" misc_feature join(9766..9915,1..163) /label="Left Border Region" /SECDrawAs="Region" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagacg ggccaacatg 181 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa 241 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 301 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc 361 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa 421 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 481 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat 541 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 601 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 661 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 721 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 781 agaagacctt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 841 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 901 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc 961 gagtcctcca ccgaaatgaa gaaaccggag ctgaccgcca cttccgtcga aaagttcctc 1021 atcgagaagt ttgatagcgt gtctgacctt atgcagttgt cggagggcga agagtcccgc 1081 gcgttcagct ttgatgttgg cgggcgcggg tacgtcctcc gcgtgaactc ttgcgctgac 1141 ggcttctata aagatcggta cgtttacagg catttcgcct cggcggccct gcctattccc 1201 gaagtcctcg acatcggcga gttttccgag agccttacgt attgtatttc ccgccgggct 1261 caaggggtga ccctccagga tctgccggaa actgagttgc ctgcggttct ccagcccgtc 1321 gccgaggcta tggacgccat cgccgcggcg gatctttctc aaacgtcggg cttcgggccg 1381 ttcggccctc aggggattgg ccagtacacc acttggaggg actttatctg cgctatcgcc 1441 gatccgcacg tgtatcattg gcaaaccgtt atggacgata cggtctccgc gagcgtggcc 1501 caggctctgg acgaactcat gttgtgggcc gaggattgcc ccgaggtaag tttctgcttc 1561 tacctttgat atatatataa taattatcat taattagtag taatataata tttcaaatat 1621 ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg 1681 tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt gcaggtccgc 1741 cacctggttc atgcggactt cggctctaat aacgtgctca ccgataatgg gcggattact 1801 gccgtcatcg actggtccga agctatgttt ggcgattcgc agtacgaagt ggcgaacatt 1861 ttcttctgga ggccttggct tgcctgcatg gaacagcaaa cgcgctactt tgagcggagg 1921 cacccggagc tggcggggtc tccccgcctc agggcctata tgttgcggat cggcctcgac 1981 cagctttacc agtccctggt tgatgggaat ttcgacgatg ctgcctgggc gcaaggccgc 2041 tgtgacgcta ttgtcaggag cggcgcgggg accgtgggcc gcactcagat cgcccggcgg 2101 tcggctgccg tttggacgga tgggtgcgtc gaagtgctcg cggacagcgg caacaggcgg 2161 ccttccaccc gcccgcgggc caaggagtga tccggaatcg cgttcgagta ttatggcatt 2221 gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg 2281 gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt 2341 ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa 2401 tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg 2461 cctctaatga ccgaagttaa tatgaggagt aaaacactga agcctgcagg ccttggcggc 2521 cgcactagtg gatcccccgg gtacgtgcag tgcagcgtga cccggtcgtg cccctctcta 2581 gagataatga gcattgcatg tctaagttat aaaaaattac cacatatttt ttttgtcaca 2641 cttgtttgaa gtgcagttta tctatcttta tacatatatt taaactttac tctacgaata 2701 atataatcta tagtactaca ataatatcag tgttttagag aatcatataa atgaacagtt 2761 agacatggtc taaaggacaa ttgagtattt tgacaacagg actctacagt tttatctttt 2821 tagtgtgcat gtgttctcct ttttttttgc aaatagcttc acctatataa tacttcatcc 2881 attttattag tacatccatt tagggtttag ggttaatggt ttttatagac taattttttt 2941 agtacatcta ttttattcta ttttagcctc taaattaaga aaactaaaac tctattttag 3001 tttttttatt taataattta gatataaaat agaataaaat aaagtgacta aaaattaaac 3061 aaataccctt taagaaatta aaaaaactaa ggaaacattt ttcttgtttc gagtagataa 3121 tgccagcctg ttaaacgccg tcgatcgacg agtctaacgg acaccaacca gcgaaccagc 3181 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc 3241 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg 3301 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc 3361 ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta 3421 ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac 3481 acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc 3541 tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag 3601 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 3661 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 3721 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 3781 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg 3841 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt 3901 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga 3961 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg 4021 ctcaccctgt tgtttggtgt tacttctgca ggtacaagct tacgcgtcct ccaccgaaat 4081 gggcaagggc gaggaactgt tcactggcgt ggtcccaatc ctggtggaac tggatggtga 4141 tgtgaacggg cacaagttct ccgtcagcgg agagggtgaa ggtgatgcca cctacggaaa 4201 gctcaccctg aagttcatct gcactaccgg aaagctccct gttccgtggc caaccctcgt 4261 caccactttc acctacggtg ttcagtgctt ctcccggtac ccagatcaca tgaagcagca 4321 tgacttcttc aagagcgcca tgcccgaagg ctacgtgcaa gaaaggacta tcttcttcaa 4381 ggatgacggg aactacaaga cacgtgccga agtcaagttc gaaggtgata ccctggtgaa 4441 ccgcatcgag ctgaaaggta agtttctgct tctacctttg atatatatat aataattatc 4501 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata 4561 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata 4621 tatgaccaaa atttgttgat gtgcaggtat cgatttcaag gaagatggaa acatcctcgg 4681 acacaagctg gagtacaact acaactccca caacgtatac atcatggccg acaagcagaa 4741 gaacggcatc aaggtgaact tcaagatcag gcacaacatc gaagatggaa gcgtgcaact 4801 ggcggaccac taccagcaga acacgcccat cggcgatggc cctgtcctgc tgccggacaa 4861 ccattacctg tccacgcaat ctgccctctc caaggacccc aacgagaaga gggaccacat 4921 ggtcctgctg gagttcgtga cggctgctgg gatcacgcat ggcatggatg aactctacaa 4981 gtgactcgtc gaccctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg 5041 ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc 5101 gccggtttcg gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa 5161 tattctccgt tcaatttact gattgtcctc gacaggcctt aacggccaga tctgctgggc 5221 cctgtggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac 5281 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt 5341 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 5401 tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt 5461 cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct 5521 agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc 5581 gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc 5641 tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc 5701 gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc 5761 gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg 5821 ttgatgtgga cgccggctta gctcatggcc aaaatccctt aacgtgagtt ttcgttccac 5881 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 5941 gtaatctgct gcttgcaaac aaaaaaacca tcgctaccag cggtggtttg tttgccggat 6001 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 6061 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 6121 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 6181 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 6241 gggggttcgt gctcacagcc cagcttggag cgaacgacct acaccgaact gagataccta 6301 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 6361 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 6421 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 6481 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggcctttcc taagggttaa 6541 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggtcg tccgagcgca 6601 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc 6661 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc 6721 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa 6781 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca 6841 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt 6901 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc 6961 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac 7021 ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca 7081 cgccccggtg aatcgtggca agccgccgct gatcgaatcc gcaaagaatc ccggcaaccg 7141 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt 7201 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggca 7261 gtattccgcc tctcgaaacg tgatcgccgg gcgggcgaag tcatccgcta cgaactgccg 7321 gacggtcatg tcgaggtgag cgcaggcccg gcgggcatgg cctccgtctg ggattatgac 7381 ctcgttctga tggccgtgtc gcaccttacg gaaagcatga atcgctaccg ggagggcaag 7441 ggtgataagc ccggtcgcgt attccgtccg catgtggccg acgttctgaa attctgtcgc 7501 cgggcggatg gcggcaagca aaaggacgat ctcgtcgaaa cctgcatccg cctgaacacg 7561 acccacgtcg ccatgcagcg caccaagaaa gcgaagaatg gccgtcttgt gactgtctcc 7621 gagggcgaag ccctgatctc gcgctacaag atcgtaaaga gcgaaaccgg gcggcccgag 7681 tacatcgaga tcgagcttgc tgattggatg taccgcgaga tcacagaagg caagaacccg 7741 gacgtgctga cggttcaccc cgattacttt ttgattgatc ccggcatcgg tcgttttctc 7801 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc 7861 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg 7921 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg 7981 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt 8041 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa agctctcttt 8101 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac 8161 attgggaacc caaagccgta cattgggaac cgggcacgca tgtaagtgac tgatataaaa 8221 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc 8281 cgcctggcct gtgcataact gtctggccag cgcacagccg aatagctgca aaaagcgcct 8341 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgtggccgct 8401 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg 8461 ccgtcgccac tcgaccgccg ccgcccacat caaggcacct cagcggtggg tatgcctgac 8521 gatgcgtgca gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt 8581 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc 8641 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca 8701 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg 8761 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc 8821 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca 8881 gtcgccctaa aacaaagtta aacatcatgc gtgaagcggt gatcgccgaa gtatcgactc 8941 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac 9001 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg 9061 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg 9121 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg 9181 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat 9241 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg 9301 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg 9361 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc 9421 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc 9481 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg 9541 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc 9601 ttggacaaga agaagatcgc ttggcctctc gcgcagatca gttggaagaa tttgtccact 9661 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc 9721 gacgccgctt cgcggcgcgg cttaactcaa gcgttagacg gaccgcccat gtggatcact 9781 ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac atggctcagt tctcaatgga 9841 aattatctgc ctaaccggct cagttctgcg tagaaaccaa catgcaagct ccaccgggtg 9901 caaagcggca gcggc //