LOCUS p6U10 10466 bp DNA circular SYN 14-MAY-2009 DEFINITION p6U10: Ligation of UbiAT-Spe-Xho* into pLH6000* ACCESSION p6U10 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p6U10 Vector molecule: pLH6000* Fragment ends: XhoI and XbaI Fragment size: 8977 Insert molecule: UbiAT-Spe-Xho* Fragment ends: SpeI and XhoI Fragment size: 1489 FEATURES Location/Qualifiers misc_feature 7..1484 /label="UBQ10" /gene="UBQ10" /product="Promotor Ubi A.thaliana" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 1113..1178 /label="Ubi cds 5`" /product="Ubi cds 5`" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 1179..1482 /label="íntron" /gene="íntron" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 1517..2542 /label="hpt" /gene="hpt" /product="Hygromycin" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 2567..2768 /label="T35S" /gene="T35S" /product="Terminator 35S" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2941..2964 /label="BR" /gene="BR" /product="right border" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 3499..4196 /label="ColE1" /gene="ColE1" /product="ColE1 ORI" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 4745..8450 /label="pVS1 ORI" /gene="pVS1 ORI" /product="pVS1 ORI" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 8634..9644 /label="Sm/Sp" /gene="Sm/Sp" /product="Strep/Spec resistence" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 10165..10188 /label="BL" /gene="BL" /product="left border" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" ORIGIN 1 tctagtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 61 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 121 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 181 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 241 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 301 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 361 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 421 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 481 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 541 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 601 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 661 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 721 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 781 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 841 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 901 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 961 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1021 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1081 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1141 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1201 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1261 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1321 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1381 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1441 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agatactagc tcgagggccc 1501 cggggggcaa taagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 1561 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 1621 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 1681 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 1741 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc 1801 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 1861 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 1921 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 1981 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 2041 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 2101 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 2161 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 2221 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 2281 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 2341 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 2401 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 2461 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 2521 tcgtccgagg gcaaaggaat agagtagatg ccgaccggag tccgcaaaaa tcaccagtct 2581 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga 2641 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta 2701 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa 2761 atccagtgac ctgcaggcat gcaagctgat ccactagagg ccatggcggc cgcactagtg 2821 gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga caggccttaa gggccagatc 2881 ttgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact atcagtgttt 2941 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 3001 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 3061 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa 3121 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg 3181 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc 3241 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg 3301 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct 3361 ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata 3421 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg 3481 ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 3541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3661 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3721 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 3781 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3841 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3901 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3961 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 4021 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4081 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4141 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4201 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4261 tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag tgagctgata 4321 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 4381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 4441 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct 4501 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg 4561 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 4621 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggggtacg tcgaggtcga 4681 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg 4741 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct 4801 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga 4861 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca 4921 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc 4981 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg 5041 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca 5101 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc 5161 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg 5221 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg 5281 ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg 5341 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc 5401 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca 5461 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg 5521 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga 5581 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt 5641 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga 5701 tgccaagctc gcggcctggc cggcgagctt ggccgctgaa gaaaccgagc gccgccgtct 5761 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat 5821 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac 5881 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc 5941 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg 6001 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc 6061 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg 6121 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc 6181 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc 6241 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc 6301 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc 6361 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa 6421 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc 6481 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc 6541 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca 6601 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg 6661 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt 6721 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa 6781 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt 6841 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag 6901 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc 6961 tgggtgatga cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg 7021 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat 7081 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc 7141 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca 7201 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct 7261 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gcgagtgtgt 7321 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc 7381 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca 7441 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc 7501 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg 7561 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg 7621 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag 7681 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg 7741 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt 7801 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg 7861 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc 7921 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg 7981 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa 8041 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc 8101 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga 8161 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa 8221 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc 8281 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta 8341 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg 8401 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ggtgggtatg 8461 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct 8521 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac 8581 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct 8641 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt 8701 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 8761 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 8821 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat 8881 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 8941 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 9001 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 9061 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 9121 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 9181 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 9241 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 9301 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 9361 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 9421 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 9481 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 9541 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 9601 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt 9661 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa 9721 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata 9781 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata 9841 gttggcgaag taatcgcaac atagcttgct tggtcgttcc gcgtgaacgt cggctcgatt 9901 gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct gcccggtgcg tcggctgatc 9961 tcacggatcg actgcttctc tcgcaacgcc atccgacgga tgatgtttaa aagtcccatg 10021 tggatcactc cgttgccccg tcgctcaccg tgttgggggg aaggtgcaca tggctcagtt 10081 ctcaatggaa attatctgcc taaccggctc agttctgcgt agaaaccaac atgcaagctc 10141 caccgggtgc aaagcggcag cggcggcagg atatattcaa ttgtaaatgg cttcatgtcc 10201 gggaaatcta catggatcag caatgagtat gatggtcaat atggagaaaa agaaagagta 10261 attaccaatt ttttttcaat tcaaaaatgt agatgtccgc agcgttatta taaaatgaaa 10321 gtacattttg ataaaacgac aaattacgat ccgtcgtatt tataggcgaa agcaataaac 10381 aaattattct aattcggaaa tctttatttc gacgtgtcta cattcacgtc caaatggggg 10441 cttagatgag aaacttcacg atcggc //