LOCUS p7D35s-UbiAtGFP 9027 bp DNA circular SYN 11-JAN-2017 DEFINITION p7D35s-UbiAtGFP (C244): Ligation of B592pUbiAt-mGFPD-ocs* into C243p7D35s* ACCESSION p7D35s-UbiAtGFP KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p7D35s-UbiAtGFP FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 169..584 /label="35S" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 605..838 /label="Bar'" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 839..1027 /label="STLS1" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 1028..1343 /label="''Bar'" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1356..1646 /label="'E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 1690..3165 /label="Pro-Ubq10At" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 2761..2764 /label="TATA" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 2794..2857 /label="Ubi cds 5`" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 2860..3163 /label="íntron" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 3191..3569 /label="GFP" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 3570..3758 /label="ST-LS1" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 3759..4096 /label="GFP" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 4105..4300 /label="OCS" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 4345..4947 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 4390..4413 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 4961..5619 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 5648..7611 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 7800..8810 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(8878..9027,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc 181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat 601 aactatgggc cccgagcgca ggcccgccga catccgccgt gccaccgagg ccgacatgcc 661 agccgtctgc accatcgtga accactacat cgagacctcc acggtcaact tccgcacaga 721 gccgcaggag cctcaggagt ggaccgacga cctcgtgagg ctgcgcgagc gctacccctg 781 gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggt 841 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata 901 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta 961 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga 1021 tgtgcaggca cgcaacgcct acgactggac ggcggagagc accgtctacg tgtccccacg 1081 ccaccagcgc accggcctgg gctccacgct ctacacccac ctgctcaaga gcctggaggc 1141 ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc aacgacccga gcgtccgtat 1201 gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg gccgcgggct tcaagcacgg 1261 caactggcac gacgtgggct tctggcagct cgacttctcc ctgcctgtcc caccccgccc 1321 ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt atggcattgg gaaaactgtt 1381 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc 1441 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat 1501 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata 1561 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc 1621 gaagttaata tgaggagtaa aacactgaag cctgcaggcc ttggcggccg cactagtgga 1681 tcccccgggt ttccttacat tctgagcctc tttccttcta atccactcat ctgcatcttc 1741 ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca ccagctcgtt 1801 tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat tcttcagatc 1861 aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg atgatctagg 1921 accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt gtatcattct 1981 tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac gagtgttaaa 2041 tatggaccag gccccaaata agatccattg atatatgaat taaataacaa gaataaatcg 2101 agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat acaaaagtca 2161 ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa aattaaaaga 2221 aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag aaattcactg 2281 attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg aaaagaaata 2341 aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac ccacggttca 2401 attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa tgctaaaaaa 2461 atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac cgttagaaat 2521 tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg gcacacacga 2581 gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata aggcaattag 2641 ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat tattagctat 2701 tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc 2761 tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat ttctcaaaat 2821 cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg tgttccttat 2881 tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt 2941 tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta gatatcatct 3001 taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt tgagttttgt 3061 cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt tctagtttgt 3121 gcgatcgaat ttgtcgatta atctgagttt ttctgattaa caggtacaag cttacgcgtc 3181 ctccataact atgggcaagg gcgaggaact gttcactggc gtggtcccaa tcctggtgga 3241 actggatggt gatgtgaacg ggcacaagtt ctccgtcagc ggagagggtg aaggtgatgc 3301 cacctacgga aagctcaccc tgaagttcat ctgcactacc ggaaagctcc ctgttccgtg 3361 gccaaccctc gtcaccactt tcacctacgg tgttcagtgc ttctcccggt acccagatca 3421 catgaagcag catgacttct tcaagagcgc catgcccgaa ggctacgtgc aagaaaggac 3481 tatcttcttc aaggatgacg ggaactacaa gacacgtgcc gaagtcaagt tcgaaggtga 3541 taccctggtg aaccgcatcg agctgaaagg taagtttctg cttctacctt tgatatatat 3601 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa 3661 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata 3721 acttttctaa tatatgacca aaatttgttg atgtgcaggt atcgatttca aggaagatgg 3781 aaacatcctc ggacacaagc tggagtacaa ctacaactcc cacaacgtat acatcatggc 3841 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc aggcacaaca tcgaagatgg 3901 aagcgtgcaa ctggcggacc actaccagca gaacacgccc atcggcgatg gccctgtcct 3961 gctgccggac aaccattacc tgtccacgca atctgccctc tccaaggacc ccaacgagaa 4021 gagggaccac atggtcctgc tggagttcgt gacggctgct gggatcacgc atggcatgga 4081 tgaactctac aagtgactcg tcgaccctgc tttaatgaga tatgcgagac gcctatgatc 4141 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 4201 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 4261 ttttatgaat aatattctcc gttcaattta ctgattgtcc tcgacaggcc ttaacggcca 4321 gatctgctgg gccctgtggt acccgatcag attgtcgttt cccgccttcg gtttaaacta 4381 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat 4441 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc 4501 caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac ttctgttcaa 4561 ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc ccttggctcg 4621 tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg 4681 acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc 4741 tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca 4801 ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc 4861 acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc tgggcagtcg 4921 ccgccgacgc cgttgatgtg gacgccggct tagctcatgg ccaaaatccc ttaacgtgag 4981 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 5041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac catcgctacc agcggtggtt 5101 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 5161 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 5221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5341 tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac ctacaccgaa 5401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5641 cctaagggtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggt 5701 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc 5761 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg 5821 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag 5881 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga 5941 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg 6001 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa 6061 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg 6121 ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg gcaacgcatc 6181 gaggcagaag cacgccccgg tgaatcgtgg caagccgccg ctgatcgaat ccgcaaagaa 6241 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag 6301 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc 6361 atggacgtgg cagtattccg cctctcgaaa cgtgatcgcc gggcgggcga agtcatccgc 6421 tacgaactgc cggacggtca tgtcgaggtg agcgcaggcc cggcgggcat ggcctccgtc 6481 tgggattatg acctcgttct gatggccgtg tcgcacctta cggaaagcat gaatcgctac 6541 cgggagggca agggtgataa gcccggtcgc gtattccgtc cgcatgtggc cgacgttctg 6601 aaattctgtc gccgggcgga tggcggcaag caaaaggacg atctcgtcga aacctgcatc 6661 cgcctgaaca cgacccacgt cgccatgcag cgcaccaaga aagcgaagaa tggccgtctt 6721 gtgactgtct ccgagggcga agccctgatc tcgcgctaca agatcgtaaa gagcgaaacc 6781 gggcggcccg agtacatcga gatcgagctt gctgattgga tgtaccgcga gatcacagaa 6841 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgattga tcccggcatc 6901 ggtcgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg 6961 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc 7021 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg 7081 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc 7141 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga 7201 aaagctctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac 7261 cggaacccgt acattgggaa cccaaagccg tacattggga accgggcacg catgtaagtg 7321 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa 7381 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaatagctg 7441 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct 7501 atcgtggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc 7561 ggacaagccg cgccgtcgcc actcgaccgc cgccgcccac atcaaggcac ctcagcggtg 7621 ggtatgcctg acgatgcgtg cagaccgaaa ccttgcgctc gttcgccagc caggacagaa 7681 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga 7741 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta 7801 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg 7861 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca 7921 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta 7981 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gcgtgaagcg gtgatcgccg 8041 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt 8101 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata 8161 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca 8221 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag 8281 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc 8341 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg 8401 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc 8461 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg 8521 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc 8581 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg 8641 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta 8701 gacaggctta tcttggacaa gaagaagatc gcttggcctc tcgcgcagat cagttggaag 8761 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa 8821 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga cggaccgccc 8881 atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca 8941 gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag 9001 ctccaccggg tgcaaagcgg cagcggc //