LOCUS p7M2x35s-Ub_6142 10533 bp DNA circular SYN 15-DEC-2022 DEFINITION p7M2x35s-UbiZmGusXi (F388): Ligation of C906p6M2x35s-UbiZmGusXi* into E760p7M2x35s* ACCESSION p7M2x35s-Ub_6142 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p7M2x35s-UbiZmGusXi FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 174..958 /label="P2x35s'" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 976..1209 /label="Bar" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1210..1398 /label="STLS1" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 1399..1716 /label="Bar" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1734..2024 /label="'E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2072..3579 /label="'Ubi-int" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 3058..3577 /label="Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 3605..4219 /label="GusX'" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 4220..4408 /label="''Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 4409..5605 /label="''GusX" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 5611..5806 /label="OCS" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 5851..6453 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 5896..5919 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 6467..7125 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 7154..9117 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 7480..8655 /label="ParB" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 9306..10316 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(10384..10533,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagacg ggccaacatg 181 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa 241 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 301 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc 361 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa 421 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 481 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat 541 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 601 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 661 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 721 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 781 agaagacctt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 841 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 901 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc 961 gagtcctcca ccgaaatggg ccccgagcgc aggcccgccg acatccgccg tgccaccgag 1021 gccgacatgc cagccgtctg caccatcgtg aaccactaca tcgagacttc cacggtcaac 1081 ttccgcacag agccgcagga gcctcaggag tggaccgacg acctcgtgag gctgcgcgag 1141 cgctacccct ggctcgtcgc tgaggtggat ggcgaggtcg ccggcatcgc ctacgcgggc 1201 ccctggaagg taagtttctg cttctacctt tgatatatat ataataatta tcattaatta 1261 gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt 1321 gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca 1381 aaatttgttg atgtgcaggc acgcaacgcc tacgactgga cggcggagag caccgtctac 1441 gtgtccccac gccaccagcg caccggcctg ggctccacgc tctacaccca cctgctcaag 1501 agcctggagg cccagggctt caagtccgtg gtcgctgtga tcggcctccc caacgacccg 1561 agcgtccgta tgcacgaggc cctcggctac gcgccccgcg ggatgctgag ggccgcgggc 1621 ttcaagcacg gcaactggca cgacgtgggc ttctggcagc tcgacttctc cctgcctgtc 1681 ccaccccgcc cggtgctccc cgtcaccgaa atctgatccg gaatcgcgtt cgagtattat 1741 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt 1801 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga 1861 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt 1921 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa 1981 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc tgcaggcctt 2041 ggcggccgca ctagtggatc ccccgggtac gtgcagtgca gcgtgacccg gtcgtgcccc 2101 tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt 2161 gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta 2221 cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga 2281 acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta 2341 tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact 2401 tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat 2461 ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta 2521 ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa 2581 ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt 2641 agataatgcc agcctgttaa acgccgtcga tcgacgagtc taacggacac caaccagcga 2701 accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg tcgctgcctc 2761 tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg gcatccagaa 2821 attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc ctcctctcac 2881 ggcaccggca gctacggggg attcctttcc caccgctcct tcgctttccc ttcctcgccc 2941 gccgtaataa atagacaccc cctccacacc ctctttcccc aacctcgtgt tgttcggagc 3001 gcacacacac acaaccagat ctcccccaaa tccacccgtc ggcacctccg cttcaaggta 3061 cgccgctcgt cctccccccc cccccctctc taccttctct agatcggcgt tccggtccat 3121 ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag 3181 atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc 3241 taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg 3301 gatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg 3361 catatgcagc atctattcat atgctctaac cttgagtacc tatctattat aataaacaag 3421 tatgttttat aattattttg atcttgatat acttggatga tggcatatgc agcagctata 3481 tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg 3541 tcgatgctca ccctgttgtt tggtgttact tctgcaggta caagcttacg cgtcctccac 3601 cgaaatgctc cgccccgtgg agaccccaac tcgtgagatc aagaagctgg acggcttgtg 3661 ggccttctcc cttgacaggg aaaactgcgg aattgatcag agatggtggg agtccgctct 3721 ccaagagtct cgcgccatcg ccgtccctgg ttcgttcaac gaccagttcg cagacgctga 3781 catccgcaat tacgccggca acgtttggta tcagagggaa gtcttcatcc cgaaggggtg 3841 ggccggacag cgcattgtcc tgcgctttga tgcggtgacc cactacggaa aagtctgggt 3901 taacaaccaa gaagtgatgg agcaccaggg cggatacaca cccttcgaag ccgacgtcac 3961 cccatacgtg atcgccggca agtccgtccg tatcaccgtt tgcgtgaaca acgagttgaa 4021 ttggcagacc atcccaccag gcatggtgat tacggacgag aacggtaaga agaagcaaag 4081 ctatttccac gacttcttca actacgccgg aatccacagg agcgtgatgc tctacaccac 4141 tcctaacacc tgggtggacg atatcaccgt cgttacccat gtcgcccagg actgcaacca 4201 cgcctccgtt gactggcagg taagtttctg cttctacctt tgatatatat ataataatta 4261 tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta 4321 tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa 4381 tatatgacca aaatttgttg atgtgcaggt ggtggccaat ggcgacgttt cagtcgagct 4441 tagagatgcc gaccagcaag tggtcgcaac tggacaggga acctccggca ccctccaagt 4501 ggtcaacccg cacctgtggc agcccggtga gggctacctc tacgagttgt gcgttacagc 4561 taagagtcag accgagtgcg acatttaccc actgcgcgtg ggaatccgtt ctgtcgccgt 4621 gaagggcgag cagttcctca tcaaccacaa gcccttctac ttcacggggt ttggccgcca 4681 cgaggacgcc gatcttaggg gaaagggctt cgacaacgtc ttgatggttc acgaccacgc 4741 tctcatggac tggatcggtg ccaactccta ccgcaccagc cactaccctt acgccgagga 4801 gatgctggac tgggcggatg aacacggaat tgtggtcatc gacgagaccg ctgctgtggg 4861 cttcaacctc tcgcttggga tcggattcga ggccggaaat aagcccaagg agctgtactc 4921 cgaggaggcc gtcaacggcg aaactcagca ggctcacctc caggccatta aggagttgat 4981 cgcaagagac aagaaccatc caagcgtggt tatgtggtcc atcgccaacg agccggacac 5041 ccgtccccag ggagctaggg agtacttcgc cccactcgcc gaggctaccc gcaagctcga 5101 tcccacccgc cctatcactt gcgtcaacgt gatgttctgc gacgcccaca ccgacacaat 5161 ttcagacctt ttcgacgtct tgtgcctcaa ccgttattac ggctggtacg tgcagagcgg 5221 tgatctggag accgccgaaa aggttttgga gaaggagctg ctcgcgtggc aagagaagct 5281 ccaccagccg atcatcatca ccgagtacgg ggtggacacg ctggccggac tccactccat 5341 gtataccgat atgtggtctg aggaatacca gtgcgcttgg cttgacatgt accacagggt 5401 gttcgaccgt gtgtcggccg tggttggaga gcaagtctgg aattttgcag acttcgccac 5461 cagccagggc attttgagag tgggaggcaa caagaaaggt atcttcactc gtgacaggaa 5521 gccaaagtcc gctgccttcc tgctccagaa gcgctggacc ggcatgaact tcggggagaa 5581 gccccagcaa ggaggcaagc agtaagtcga ccctgcttta atgagatatg cgagacgcct 5641 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt 5701 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta 5761 tcgtattttt atgaataata ttctccgttc aatttactga ttgtcctcga caggccttaa 5821 cggccagatc tgctgggccc tgtggtaccc gatcagattg tcgtttcccg ccttcggttt 5881 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat 5941 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt 6001 gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct 6061 gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt 6121 ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg 6181 tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc 6241 gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc 6301 gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg 6361 aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg 6421 cagtcgccgc cgacgccgtt gatgtggacg ccggcttagc tcatggccaa aatcccttaa 6481 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 6541 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccatc gctaccagcg 6601 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 6661 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 6721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6841 cagcggtcgg gctgaacggg gggttcgtgc tcacagccca gcttggagcg aacgacctac 6901 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6961 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 7021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 7081 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 7141 gcctttccta agggttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt 7201 gccggtcgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg 7261 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg 7321 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta 7381 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg 7441 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac 7501 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac 7561 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc 7621 gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa 7681 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag ccgccgctga tcgaatccgc 7741 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc 7801 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc 7861 agcatcatgg acgtggcagt attccgcctc tcgaaacgtg atcgccgggc gggcgaagtc 7921 atccgctacg aactgccgga cggtcatgtc gaggtgagcg caggcccggc gggcatggcc 7981 tccgtctggg attatgacct cgttctgatg gccgtgtcgc accttacgga aagcatgaat 8041 cgctaccggg agggcaaggg tgataagccc ggtcgcgtat tccgtccgca tgtggccgac 8101 gttctgaaat tctgtcgccg ggcggatggc ggcaagcaaa aggacgatct cgtcgaaacc 8161 tgcatccgcc tgaacacgac ccacgtcgcc atgcagcgca ccaagaaagc gaagaatggc 8221 cgtcttgtga ctgtctccga gggcgaagcc ctgatctcgc gctacaagat cgtaaagagc 8281 gaaaccgggc ggcccgagta catcgagatc gagcttgctg attggatgta ccgcgagatc 8341 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gattgatccc 8401 ggcatcggtc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga 8461 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt 8521 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag 8581 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca 8641 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa 8701 ggtcgaaaag ctctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt 8761 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg ggcacgcatg 8821 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt 8881 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa 8941 tagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt 9001 cggcctatcg tggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca 9061 gggcgcggac aagccgcgcc gtcgccactc gaccgccgcc gcccacatca aggcacctca 9121 gcggtgggta tgcctgacga tgcgtgcaga ccgaaacctt gcgctcgttc gccagccagg 9181 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac 9241 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt 9301 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 9361 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg 9421 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 9481 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgcgt gaagcggtga 9541 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac 9601 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca 9661 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt 9721 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg 9781 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg 9841 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca 9901 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg 9961 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc 10021 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg 10081 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg 10141 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg 10201 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctctcgc gcagatcagt 10261 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc 10321 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagacgga 10381 ccgcccatgt ggatcactcc gttgccccgt cgctcaccgt gttgggggga aggtgcacat 10441 ggctcagttc tcaatggaaa ttatctgcct aaccggctca gttctgcgta gaaaccaaca 10501 tgcaagctcc accgggtgca aagcggcagc ggc //