LOCUS p9DN-35s-ocs 7219 bp DNA circular SYN 29-AUG-2024 DEFINITION p9DN-35s-ocs (G338): Ligation of B927-Xba-Kpn* into D010p6D35s* ACCESSION p9DN-35s-ocs KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p9DN-35s-ocs FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 169..466 /label="P-NOS" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 485..783 /label="NptIIo'" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 780..783 /label="splice" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 784..970 /label="'intron" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 969..972 /label="splice" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 971..1474 /label="''NptIIo'" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1488..1778 /label="'E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 1833..2255 /label="35S-Pro" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 2221..2227 /label="TATA" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 2302..2497 /label="OCS" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2537..3139 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 2582..2605 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 3153..3811 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 3840..5803 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 4166..5341 /label="ParB" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 5992..7002 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(7070..7219,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagatc atgagcggag 181 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg 241 gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg 301 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat 361 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt 421 atccaattag agtctcatat tcactctcaa tccaaataat ctgcacctcg agtcctccat 481 aactatggga gcatggattg agcaggatgg cctccatgct ggtagccccg ctgcttgggt 541 ggagcgcctc tttggctacg actgggccca acagaccatt gggtgcagtg atgccgctgt 601 cttccgtttg agcgcccagg gcaggccagt tctcttcgtg aagactgacc tctccggtgc 661 cctcaacgag ttgcaggacg aagctgcccg cctctcatgg cttgccacca ccggagtccc 721 ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga gactggctgc tcttgggcga 781 ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat 841 ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct 901 gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt 961 tgatgtgcag gtgccaggcc aagacctcct gagctcccac ctggccccag ccgagaaggt 1021 ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg gacccagcca cctgcccctt 1081 cgaccaccag gccaagcaca ggatcgaacg tgcacgcact aggatggagg ccggccttgt 1141 ggaccaggat gaccttgacg aggagcacca gggccttgct cccgccgagc tgttcgcccg 1201 cctcaaggct agaatgcctg acggagagga cttggtggtc acccacgggg atgcctgcct 1261 ccccaacatc atggttgaga acggacgctt ctctggcttc atcgactgcg gtcgtctggg 1321 cgtggccgac cgctaccaag acatcgccct ggccaccagg gacatcgctg aggaacttgg 1381 aggggagtgg gccgatcgtt tcttggtcct ctacggcatt gctgcccccg actcccagag 1441 gatcgccttc taccgcctgc ttgacgagtt cttctgaacg cgttcgagta ttatggcatt 1501 gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg 1561 gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt 1621 ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa 1681 tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg 1741 cctctaatga ccgaagttaa tatgaggagt aaaacactga agcctgcagg catgcaagct 1801 gatccactag aggccatggc ggccgcacta gatcaacatg gtggagcacg acactctcgt 1861 ctactccaag aatatcaaag atacagtctc agaagaccag agggctattg agacttttca 1921 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat 1981 cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc catcattgcg ataaaggaaa 2041 ggctatcgtt caagatgcct ctaccgacag tggtcccaaa gatggacccc cacccacgag 2101 gaacatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga 2161 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc 2221 tatataagga agttcatttc atttggagag gacactagtt ggatcccccg ggctgcagga 2281 attcaagctt acgcgtgtcg accctgcttt aatgagatat gcgagacgcc tatgatcgca 2341 tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag 2401 atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt 2461 tatgaataat attctccgtt caatttactg attgtcctcg acaggcctta agggccagat 2521 cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac tatcagtgtt 2581 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 2641 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 2701 gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa 2761 acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc 2821 ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg 2881 cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga tctgcttgtc 2941 gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca caggatgatc 3001 tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact ccacgaaaat 3061 atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac 3121 gccgttgatg tggacgccgg cttagctcat ggccaaaatc ccttaacgtg agttttcgtt 3181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 3241 gcgcgtaatc tgctgcttgc aaacaaaaaa accatcgcta ccagcggtgg tttgtttgcc 3301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 3361 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 3421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 3481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 3541 aacggggggt tcgtgctcac agcccagctt ggagcgaacg acctacaccg aactgagata 3601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 3721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttcctaaggg 3841 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gtcgtccgag 3901 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 3961 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 4021 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 4081 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 4141 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 4201 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg 4261 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 4321 tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga 4381 agcacgcccc ggtgaatcgt ggcaagccgc cgctgatcga atccgcaaag aatcccggca 4441 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 4501 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 4561 ggcagtattc cgcctctcga aacgtgatcg ccgggcgggc gaagtcatcc gctacgaact 4621 gccggacggt catgtcgagg tgagcgcagg cccggcgggc atggcctccg tctgggatta 4681 tgacctcgtt ctgatggccg tgtcgcacct tacggaaagc atgaatcgct accgggaggg 4741 caagggtgat aagcccggtc gcgtattccg tccgcatgtg gccgacgttc tgaaattctg 4801 tcgccgggcg gatggcggca agcaaaagga cgatctcgtc gaaacctgca tccgcctgaa 4861 cacgacccac gtcgccatgc agcgcaccaa gaaagcgaag aatggccgtc ttgtgactgt 4921 ctccgagggc gaagccctga tctcgcgcta caagatcgta aagagcgaaa ccgggcggcc 4981 cgagtacatc gagatcgagc ttgctgattg gatgtaccgc gagatcacag aaggcaagaa 5041 cccggacgtg ctgacggttc accccgatta ctttttgatt gatcccggca tcggtcgttt 5101 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 5161 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 5221 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 5281 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 5341 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaagctct 5401 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 5461 gtacattggg aacccaaagc cgtacattgg gaaccgggca cgcatgtaag tgactgatat 5521 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 5581 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaatagc tgcaaaaagc 5641 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgtggc 5701 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 5761 cgcgccgtcg ccactcgacc gccgccgccc acatcaaggc acctcagcgg tgggtatgcc 5821 tgacgatgcg tgcagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg 5881 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga 5941 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca 6001 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc 6061 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa 6121 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag 6181 ggcagtcgcc ctaaaacaaa gttaaacatc atgcgtgaag cggtgatcgc cgaagtatcg 6241 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 6301 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 6361 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 6421 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 6481 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 6541 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 6601 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 6661 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 6721 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 6781 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 6841 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct 6901 tatcttggac aagaagaaga tcgcttggcc tctcgcgcag atcagttgga agaatttgtc 6961 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca 7021 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gacggaccgc ccatgtggat 7081 cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa 7141 tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg 7201 ggtgcaaagc ggcagcggc //