LOCUS p9M2x35s 7086 bp DNA circular SYN 15-JUN-2023 DEFINITION p9M2x35s (F652): Ligation of NptE9-Xho-Bam* into E760p7M2x35s* ACCESSION p9M2x35s KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p9M2x35s FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 174..958 /label="P2x35s'" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 976..1274 /label="NptIIo" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" CDS 1275..1461 /label="'intron" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 1462..1965 /label="NptIIo'" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1623 /label="Change to T" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 1987..2276 /label="''E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2404..3006 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 2449..2472 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 3020..3678 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 3707..5670 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 4033..5208 /label="ParB" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 5859..6869 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(6937..7086,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagacg ggccaacatg 181 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa 241 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 301 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc 361 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa 421 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 481 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat 541 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 601 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 661 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 721 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 781 agaagacctt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 841 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 901 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc 961 gagtcctcca ccgaaatggg agcatggatt gagcaggatg gcctccatgc tggtagcccc 1021 gctgcttggg tggagcgcct ctttggctac gactgggccc aacagaccat tgggtgcagt 1081 gatgccgctg tgttccgttt gagcgcccag ggcaggccag ttctcttcgt caagactgac 1141 ctctccggtg ccctcaacga gttgcaggac gaagctgccc gcctctcatg gcttgccacc 1201 accggagtcc cttgcgctgc cgtgttggac gttgtcacag aggccggaag agactggctg 1261 ctcttgggcg aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa 1321 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca 1381 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga 1441 ccaaaatttg ttgatgtgca ggtgccaggc caagacctcc tgagctccca cctggcccca 1501 gccgagaagg tgtccatcat ggcagacgcc atgcgtcgcc tccacaccct ggacccagcc 1561 acctgcccct tcgaccacca ggccaagcac aggatcgaac gtgcacgcac taggatggag 1621 gctggccttg tggaccagga tgaccttgac gaggagcacc agggccttgc tcccgccgag 1681 ctgttcgccc gcctcaaggc tagaatgcct gacggagagg acttggtggt cacccacggg 1741 gatgcctgcc tccccaacat catggttgag aacggacgct tctctggctt catcgactgc 1801 ggtcgtctgg gcgtggccga ccgctaccaa gacatcgccc tggccaccag ggacatcgct 1861 gaggaacttg gaggggagtg ggccgatcgt ttcttggtcc tctacggcat tgctgccccc 1921 gactcccaga ggatcgcctt ctaccgcctg cttgacgagt tcttctgatc cggaatcgcg 1981 ttcgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt 2041 tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga 2101 gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct 2161 tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa 2221 aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag 2281 cctgcaggcc ttggcggccg cagcactagt cgtggatcct cacccgggag gtgtacaggt 2341 gaattctgca agcttgtcgt cgacaggcct taacggccag atctgctggg ccctgtggta 2401 cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga caggatatat 2461 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg 2521 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg 2581 gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc 2641 tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg 2701 agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa 2761 tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg 2821 acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc 2881 tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc 2941 aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc gttgatgtgg 3001 acgccggctt agctcatggc caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3121 tgcttgcaaa caaaaaaacc atcgctacca gcggtggttt gtttgccgga tcaagagcta 3181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 3241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3421 tgctcacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttc ctaagggtta atgaggtaaa 3721 gagaaaatga gcaaaagcac aaacacgcta agtgccggtc gtccgagcgc acgcagcagc 3781 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag 3841 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt 3901 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat 3961 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac 4021 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg 4081 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc 4141 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag 4201 aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt 4261 gaatcgtggc aagccgccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc 4321 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg 4381 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc agtattccgc 4441 ctctcgaaac gtgatcgccg ggcgggcgaa gtcatccgct acgaactgcc ggacggtcat 4501 gtcgaggtga gcgcaggccc ggcgggcatg gcctccgtct gggattatga cctcgttctg 4561 atggccgtgt cgcaccttac ggaaagcatg aatcgctacc gggagggcaa gggtgataag 4621 cccggtcgcg tattccgtcc gcatgtggcc gacgttctga aattctgtcg ccgggcggat 4681 ggcggcaagc aaaaggacga tctcgtcgaa acctgcatcc gcctgaacac gacccacgtc 4741 gccatgcagc gcaccaagaa agcgaagaat ggccgtcttg tgactgtctc cgagggcgaa 4801 gccctgatct cgcgctacaa gatcgtaaag agcgaaaccg ggcggcccga gtacatcgag 4861 atcgagcttg ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg 4921 acggttcacc ccgattactt tttgattgat cccggcatcg gtcgttttct ctaccgcctg 4981 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc 5041 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca 5101 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc 5161 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag 5221 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aagctctctt tcctgtggat 5281 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac 5341 ccaaagccgt acattgggaa ccgggcacgc atgtaagtga ctgatataaa agagaaaaaa 5401 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc 5461 tgtgcataac tgtctggcca gcgcacagcc gaatagctgc aaaaagcgcc tacccttcgg 5521 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgtggccgc tggccgctca 5581 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca 5641 ctcgaccgcc gccgcccaca tcaaggcacc tcagcggtgg gtatgcctga cgatgcgtgc 5701 agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc 5761 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat 5821 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca 5881 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg 5941 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg 6001 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta 6061 aaacaaagtt aaacatcatg cgtgaagcgg tgatcgccga agtatcgact caactatcag 6121 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg 6181 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga 6241 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg 6301 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg 6361 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca 6421 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc 6481 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg 6541 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact 6601 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt 6661 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc 6721 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag 6781 aagaagatcg cttggcctct cgcgcagatc agttggaaga atttgtccac tacgtgaaag 6841 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 6901 tcgcggcgcg gcttaactca agcgttagac ggaccgccca tgtggatcac tccgttgccc 6961 cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg 7021 cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc 7081 agcggc //