LOCUS p9iTE9 9433 bp DNA circular SYN 31-MAY-2013 DEFINITION p9iTE9: Ligation of 9i-Xho-Mlu* into 257p7-TE9* ACCESSION p9iTE9 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/binaries/p9iTE9 Vector molecule: 257p7-TE9* Fragment ends: MluI and XhoI Fragment size: 8426 Insert molecule: 9i-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 1007 Primer for gene synthesis 35RT CTTCGCAAGACCCTTCCTCT RBCS-TR2 CACAGTAAATTACAAGCACAAC Sequencing primers 35S GCACAATCCCACTATCCTT RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /label="LB" /gene="LB" /product="Left Border" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 309..724 /label="35S" /gene="35S" /product="35S-Promotor" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 741..777 /label="NptII" /gene="NptII" /product="Kanamycin resistance" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_signal 746 /label="mut to C" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_signal 749 /label="mut to C" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 778..966 /label="int" /gene="int" /product="Intron StLS1" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 967..1733 /label="NptII" /gene="NptII" /product="Kanamycin-Resistenz" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1744..2034 /label="'E9" /gene="'E9" /product="Terminator Rbcs-E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2210..2233 /label="RB" /gene="RB" /product="Right Border" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 2768..3465 /label="ColE1" /gene="ColE1" /product="ColE1 ORI" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 4014..7719 /label="pVS1" /gene="pVS1" /product="origin of replication for agrobacteria" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 7903..8913 /label="Sm/Sp" /gene="Sm/Sp" /product="Strep/Spec resistence" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtggccacc atgggcgcct ggattgaaca agatggattg cacgcaggta 781 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa 841 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag 901 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat 961 gtgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 1021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 1081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 1141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 1201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 1261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 1321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 1381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 1441 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 1501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 1561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 1621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 1681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtt 1741 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta 1801 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt 1861 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta 1921 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa 1981 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc 2041 tgcaggcatg caagctgatc cactagaggc catggcggcc gcactagtgg atcccccggg 2101 atgcatgaat tcaagcttat cgatgtcgac aggccttaag ggccagatct tgggcccggt 2161 acccgatcag attgtcgttt cccgccttcg gtttaaacta tcagtgtttg acaggatata 2221 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 2281 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg 2341 ggagtgcttg gcattccgtg cgataatgac ttctgttcaa ccacccaaac gtcggaaagc 2401 ctgacgacgg agcagcattc caaaaagatc ccttggctcg tctgggtcgg ctagaaggtc 2461 gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg acgtagcgca gcgccgaaaa 2521 atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc tgcttgtcgc tctttcggcc 2581 gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca ggatgatctg gcgcgagttg 2641 ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag 2701 caagatatcg cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg 2761 gacgccgaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 2821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 3541 atcccctgat tctgtggata accgattacc gcctttgagt gagctgatac cgctcgccgc 3601 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 3661 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca 3721 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg 3781 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 3841 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 3901 tttcaccgtc atcaccgaaa cgcgcgaggc aggggtacgt cgaggtcgat ccaacccctc 3961 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg 4021 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt 4081 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc 4141 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag 4201 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag 4261 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc 4321 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta 4381 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg 4441 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc 4501 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcg 4561 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc 4621 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat 4681 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg 4741 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag 4801 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt 4861 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg 4921 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctcg 4981 cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat 5041 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa 5101 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg 5161 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga 5221 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga 5281 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc 5341 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt 5401 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat 5461 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag 5521 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt 5581 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt 5641 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga 5701 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa 5761 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca 5821 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc 5881 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc 5941 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa 6001 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca 6061 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt 6121 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc 6181 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac 6241 ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca 6301 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg 6361 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt 6421 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc 6481 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca 6541 gacgggcacg tagaggtttc cgcagggccg gccggcatgg cgagtgtgtg ggattacgac 6601 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag 6661 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg 6721 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc 6781 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc 6841 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag 6901 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg 6961 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc 7021 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc 7081 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg 7141 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg 7201 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt 7261 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt 7321 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac 7381 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa 7441 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc 7501 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct 7561 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct 7621 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg 7681 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg gtgggtatgc ctgacgatgc 7741 gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg 7801 ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg 7861 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg 7921 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt 7981 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac 8041 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc 8101 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta 8161 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 8221 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 8281 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 8341 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 8401 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 8461 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 8521 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 8581 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 8641 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 8701 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 8761 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga 8821 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg 8881 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc 8941 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc 9001 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca 9061 aattgggaga tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt 9121 aatcgcaaca tagcttgctt ggtcgttccg cgtgaacgtc ggctcgattg tacctgcgtt 9181 caaatacttt gcgatcgtgt tgcgcgcctg cccggtgcgt cggctgatct cacggatcga 9241 ctgcttctct cgcaacgcca tccgacggat gatgtttaaa agtcccatgt ggatcactcc 9301 gttgccccgt cgctcaccgt gttgggggga aggtgcacat ggctcagttc tcaatggaaa 9361 ttatctgcct aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca 9421 aagcggcagc ggc //