LOCUS F651p6D35s-C9wt-U6GmNhe 13245 bp DNA circular SYN 15-JUN-2023 DEFINITION Ligation of D784p7D35s-C9wt-U6GmNhe* into D010p6D35s* ACCESSION F651p6D35s-C9wt-U6GmNhe KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /SECDrawAs="Gene" misc_feature 169..584 /label="35S" /SECDrawAs="Region" misc_feature 600..604 /label="Kozak" /SECDrawAs="Region" CDS 605..1174 /label="hptII" /SECDrawAs="Gene" misc_feature 1175..1363 /label="int" /SECDrawAs="Gene" CDS 1364..1819 /label="'hptII''" /SECDrawAs="Gene" misc_feature 1830..2120 /label="'E9" /SECDrawAs="Region" misc_feature 2146..3621 /label="Pro-Ubq10At" /SECDrawAs="Region" CDS 3250..3313 /label="Ubi cds 5`" /SECDrawAs="Gene" misc_feature 3316..3619 /label="íntron" /SECDrawAs="Region" CDS 3643..7839 /label="'Cas9WT" /SECDrawAs="Gene" misc_feature 3649..3669 /label="NLS" /SECDrawAs="Region" misc_feature 7789..7836 /label="NLS" /SECDrawAs="Region" misc_feature 7845..8098 /label="NOS-T" /SECDrawAs="Region" misc_feature 8108..8412 /label="U6Gm" /SECDrawAs="Region" CDS 8413..8439 /label="targetRNA" /SECDrawAs="Gene" CDS 8440..8469 /label="hairpin" /SECDrawAs="Gene" misc_feature 8440..8522 /label="gRNA" /SECDrawAs="Region" misc_feature 8563..9165 /label="Right Border Region" /SECDrawAs="Region" misc_feature 8608..8631 /label="RB" /SECDrawAs="Gene" misc_feature 9179..9837 /label="'ColE1''" /SECDrawAs="Region" misc_feature 9866..11829 /label="''pVS1 ORI'''" /SECDrawAs="Region" CDS 10192..11367 /label="ParB" /SECDrawAs="Gene" CDS 12018..13028 /label="strep/spec" /SECDrawAs="Gene" misc_feature join(13096..13245,1..163) /label="Left Border Region" /SECDrawAs="Region" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc 181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat 601 aactatgaag aaaccggagc tgaccgccac ttccgtcgaa aagttcctca tcgagaagtt 661 tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa gagtcccgcg cgttcagctt 721 tgatgttggc gggcgcgggt acgtcctccg cgtgaactct tgcgctgacg gcttctataa 781 agatcggtac gtttacaggc atttcgcctc ggcggccctg cctattcccg aagtcctcga 841 catcggcgag ttttccgaga gccttacgta ttgtatttcc cgccgggctc aaggggtgac 901 cctccaggat ctgccggaaa ctgagttgcc tgcggttctc cagcccgtcg ccgaggctat 961 ggacgccatc gccgcggcgg atctttctca aacgtcgggc ttcgggccgt tcggccctca 1021 ggggattggc cagtacacca cttggaggga ctttatctgc gctatcgccg atccgcacgt 1081 gtatcattgg caaaccgtta tggacgatac ggtgtccgcg agcgtggccc aggctctgga 1141 cgaactcatg ttgtgggccg aggattgccc cgaggtaagt ttctgcttct acctttgata 1201 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa 1261 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat 1321 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggtccgcc acctggttca 1381 tgcggacttc ggctctaata acgtgctcac cgataatggg cggattactg ccgtcatcga 1441 ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg gcgaacattt tcttctggag 1501 gccttggctt gcctgcatgg aacagcaaac gcgctacttt gagcggaggc acccggagct 1561 ggcggggtca ccccgcctca gggcctatat gttgcggatc ggcctcgacc agctttacca 1621 gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg caaggccgct gtgacgctat 1681 tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc gcccggcggt cggctgccgt 1741 ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc aacaggcggc cttccacccg 1801 cccgcgggcc aaggagtgaa cgcgttcgag tattatggca ttgggaaaac tgtttttctt 1861 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg 1921 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa 1981 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat 2041 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt 2101 aatatgagga gtaaaacact gaagcctgca ggccttggcg gccgctttcc ttacattctg 2161 agcctctttc cttctaatcc actcatctgc atcttcttgt gtccttacta atacctcatt 2221 ggttccaaat tccctccctt taagcaccag ctcgtttctg ttcttccaca gcctcccaag 2281 tatccaaggg actaaagcct ccacattctt cagatcagga tattcttgtt taagatgttg 2341 aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc cttcttcata 2401 gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat taatgaaaaa 2461 atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc caaataagat 2521 ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac ttgccttttt 2581 taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa tcaataatca 2641 tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca caatatgtta 2701 tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca cttgcattag 2761 ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt ctagaaaata 2821 cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt tcagctccac 2881 cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa 2941 tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat 3001 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac 3061 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac 3121 aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg 3181 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc 3241 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc 3301 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt 3361 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga 3421 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag 3481 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg 3541 atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct 3601 gagtttttct gattaacagg tactagtgga tcctccataa ctatggcccc caagaagaag 3661 cgcaaagtgg gaatccacgg agctccagac aagaagtaca gcattggtct ggacatcggg 3721 accaactccg tcggctgggc cgttatcacc gatgagtaca aagtgccttc taagaagttc 3781 aaagtcctcg gaaacactga caggcacagc atcaagaaga acttgattgg cgcccttctc 3841 ttcgactccg gcgaaaccgc tgaggccacc cgtctgaagc gcaccgccag aaggcgctac 3901 acccgtcgca agaataggat ctgctacctc caggagatct tcagcaacga gatggccaaa 3961 gtggacgatt ccttctttca ccgtttggag gagagcttcc ttgtcgagga ggacaagaag 4021 cacgagcgcc atcccatctt cggaaacatt gttgacgagg tggcttacca cgagaagtat 4081 ccaactatct accacctcag aaagaagctg gtggactcca ccgacaaggc cgatttgagg 4141 ctcatctacc ttgccctcgc tcacatgatc aagttccgcg gtcacttcct gattgagggc 4201 gacctcaacc ccgacaactc tgacgtcgat aagttgttca tccaactggt ccagacctac 4261 aaccagcttt tcgaggagaa tcctatcaac gcctccgggg ttgacgccaa ggctattctc 4321 agcgcccgtc tgtccaagag ccgcaggctt gagaacttga tcgcccagct cccaggagag 4381 aagaagaacg gcctcttcgg aaacctgatc gctctctcct tgggccttac ccctaacttc 4441 aagtctaatt ttgacctcgc cgaggacgcc aagctgcaac tcagcaagga cacctacgat 4501 gacgacttgg acaacctgct cgcccagatc ggcgatcagt acgctgacct tttcttggcc 4561 gccaagaacc tctccgacgc tattctgctc tccgacatcc ttcgcgtgaa cactgagatc 4621 accaaggccc cactgagcgc ctccatgatc aagcgttacg acgaacacca ccaagatttg 4681 accctcctca aggctcttgt cagacagcag ctgcccgaga agtacaagga gattttcttc 4741 gaccagagca agaacggata cgccggttac atcgacggcg gggcctccca agaggagttc 4801 tacaagttca tcaagcccat cttggagaag atggacggca ccgaagagct gctcgtgaag 4861 ctcaaccgcg aggatctttt gaggaagcag cgtaccttcg acaacggatc tattccccac 4921 cagatccatc tcggcgagct gcacgctatc ctccgcaggc aagaggactt ctatccattc 4981 cttaaggaca atcgcgagaa gattgaaaag atcctcactt ttagaatccc ttactacgtc 5041 ggacccttgg cccgtggcaa cagccgcttc gcctggatga ccaggaagtc cgaggaaacc 5101 atcaccccat ggaacttcga ggaggtggtt gacaaggggg ccagcgctca gtccttcatt 5161 gagcgcatga ccaacttcga taagaacctg cccaacgaga aagtcctccc aaagcactcc 5221 cttctgtacg agtacttcac tgtctacaat gagttgacca aagtgaagta cgtgaccgag 5281 ggtatgcgta agcctgcctt cctcagcggc gagcagaaga aggccatcgt tgacctcctg 5341 ttcaagacca acaggaaagt caccgtgaag cagcttaagg aggactactt caagaagatc 5401 gagtgctttg actctgtcga gatcagcgga gtggaggatc gcttcaacgc ttccttgggc 5461 acttaccacg acctcctgaa gattatcaag gacaaggact tcctcgacaa cgaggagaac 5521 gaagatatct tggaggacat cgttcttacc ctcaccctgt tcgaggacag agagatgatt 5581 gaggaacgcc tcaagaccta cgcccacttg ttcgacgata aagtcatgaa gcaacttaag 5641 cgtaggcgct acaccggatg gggcaggctg tcccgtaagc tcatcaacgg tatccgcgac 5701 aagcagagcg gcaagactat tctcgacttc ttgaagtccg acggattcgc caatcgcaac 5761 ttcatgcagc tgatccacga cgattctctt accttcaagg aggacatcca aaaggctcaa 5821 gtgtccggcc agggcgacag cctccacgag cacatcgcca acctggccgg gagccccgct 5881 attaagaagg gaatcctcca gaccgtcaag gtggttgacg agttggtcaa ggtgatgggc 5941 agacataagc cagagaacat cgtcatcgag atggccaggg aaaaccagac cacccagaag 6001 ggacagaaga actcccgtga gcgcatgaag aggattgagg agggcatcaa ggagcttggt 6061 tcccagatcc tcaaggagca ccccgtggaa aacacccaac tccagaatga gaagctgtat 6121 ttgtactacc ttcagaacgg gcgcgatatg tacgttgacc aagagctgga catcaaccgc 6181 ctcagcgact acgacgtgga tcacattgtc cctcagtcct ttctcaagga cgactctatc 6241 gacaacaagg tgttgactag aagcgataag aaccgcggca agtccgacaa cgtcccatcc 6301 gaggaggtgg tcaagaagat gaagaattac tggaggcagc tgcttaacgc caagctcatc 6361 acccagcgca agttcgacaa cttgaccaag gccgagcgtg gaggcctcag cgaactggac 6421 aaggctggat tcatcaagag gcaacttgtt gaaacccgcc agattaccaa gcacgtggcc 6481 cagatcctcg actcccgtat gaacactaag tacgatgaga acgacaagct gatccgcgag 6541 gtcaaagtga ttaccctcaa gagcaagctc gtgtctgact tcagaaagga cttccaattc 6601 tacaaggtta gggagatcaa caactaccac cacgcccacg atgcttacct taatgccgtg 6661 gtcggcaccg ccttgatcaa gaagtacccc aagctggagt ccgagttcgt gtatggtgac 6721 tacaaggtct acgacgttcg caagatgatc gctaagagcg agcaggagat tggcaaggcc 6781 accgccaagt acttcttcta ctccaacatc atgaacttct ttaagaccga gatcactttg 6841 gctaacgggg agatccgtaa gcgccctctc attgaaacca acggagaaac cggcgagatc 6901 gtgtgggaca agggcaggga cttcgccacc gtcagaaaag tgctgagcat gccccaagtc 6961 aacatcgtga agaaaaccga ggttcagact ggaggcttct ccaaggagtc tatcctccca 7021 aagcgcaatt ccgataagtt gattgcccgt aagaaggact gggaccccaa gaagtacggt 7081 ggattcgaca gcccaaccgt cgcctactcc gtgcttgtcg tggctaaagt tgagaagggc 7141 aagagcaaga agctcaagtc cgtcaaggag ctgctcggga tcaccatcat ggagcgcagc 7201 tccttcgaga agaaccctat tgatttcctt gaggccaagg gctacaagga agtgaagaag 7261 gacttgatca tcaagctccc caagtactct ctgttcgagc ttgagaacgg aaggaagcgt 7321 atgctcgcct ccgctggcga gctgcaaaag ggaaacgagt tggccctccc aagcaagtac 7381 gtcaacttcc tgtacctcgc ctcccactat gagaagctca agggcagccc cgaggacaac 7441 gaacagaagc agttgtttgt ggagcagcat aagcactacc ttgacgagat cattgagcag 7501 atcagcgagt tctccaagcg cgttatcctg gctgacgcca atctcgataa agtcctttct 7561 gcctacaaca agcacaggga caagcctatc agagagcagg ctgaaaacat tatccacttg 7621 ttcaccctca ccaacctggg tgccccagcc gccttcaagt acttcgacac taccatcgac 7681 cgcaagcgtt acacctccac caaggaagtg ctcgatgcta cccttatcca ccagagcatt 7741 actgggttgt acgaaaccag gatcgacctg tcccaactcg gcggagacaa gcgccccgcc 7801 gccaccaaga aggctggcca ggccaagaag aagaagtaac ccgggatcgt tcaaacattt 7861 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat 7921 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga 7981 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa 8041 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgct 8101 cgaattcaaa ataaatggta aaatgtcaaa tcaaaactag gctgcagtat gcagagcaga 8161 gtcatgatga tactacttac tacaccgatt cttgtgtgca gaaaaatatg ttaaaataat 8221 tgaatctttc tctagccaaa tttgacaaca atgtacaccg ttcatattga gagacgatgc 8281 ttcttgtttg ctttcggtgg aagctgcata tactcaacat tactccttca gcgagttttc 8341 caactgagtc ccacattgcc cagacctaac acggtattct tgtttataat gaaatgtgcc 8401 accacatgga ttgagacctt cgaagcttct gtggtctcag ttttagagct agaaatagca 8461 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt 8521 ttgctagcag gccttaaggg ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc 8581 cgccttcggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa 8641 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt 8701 ccatttgtat gtgcatgcca accacagggt tcccctcggg agtgcttggc attccgtgcg 8761 ataatgactt ctgttcaacc acccaaacgt cggaaagcct gacgacggag cagcattcca 8821 aaaagatccc ttggctcgtc tgggtcggct agaaggtcga gtgggctgct gtggcttgat 8881 ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg 8941 ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc agtcatcacg 9001 cgccaaagtt ccgtcacagg atgatctggc gcgagttgct ggatctcgcc ttcaatccgg 9061 gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg 9121 gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccggctta gctcatggcc 9181 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 9241 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 9301 tcgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 9361 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 9421 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 9481 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 9541 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gctcacagcc cagcttggag 9601 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 9661 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 9721 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 9781 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 9841 gccagcaacg cggcctttcc taagggttaa tgaggtaaag agaaaatgag caaaagcaca 9901 aacacgctaa gtgccggtcg tccgagcgca cgcagcagca aggctgcaac gttggccagc 9961 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc 10021 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac 10081 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc 10141 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat 10201 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa 10261 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg 10321 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc ggcgcaggcc 10381 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agccgccgct 10441 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag 10501 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc 10561 cgcgatagtc gcagcatcat ggacgtggca gtattccgcc tctcgaaacg tgatcgccgg 10621 gcgggcgaag tcatccgcta cgaactgccg gacggtcatg tcgaggtgag cgcaggcccg 10681 gcgggcatgg cctccgtctg ggattatgac ctcgttctga tggccgtgtc gcaccttacg 10741 gaaagcatga atcgctaccg ggagggcaag ggtgataagc ccggtcgcgt attccgtccg 10801 catgtggccg acgttctgaa attctgtcgc cgggcggatg gcggcaagca aaaggacgat 10861 ctcgtcgaaa cctgcatccg cctgaacacg acccacgtcg ccatgcagcg caccaagaaa 10921 gcgaagaatg gccgtcttgt gactgtctcc gagggcgaag ccctgatctc gcgctacaag 10981 atcgtaaaga gcgaaaccgg gcggcccgag tacatcgaga tcgagcttgc tgattggatg 11041 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt 11101 ttgattgatc ccggcatcgg tcgttttctc taccgcctgg cacgccgcgc cgcaggcaag 11161 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc 11221 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat 11281 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc 11341 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta 11401 gcaggggaaa aaggtcgaaa agctctcttt cctgtggata gcacgtacat tgggaaccca 11461 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac 11521 cgggcacgca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac 11581 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag 11641 cgcacagccg aatagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc 11701 gccgcttcgc gtcggcctat cgtggccgct ggccgctcaa aaatggctgg cctacggcca 11761 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg ccgcccacat 11821 caaggcacct cagcggtggg tatgcctgac gatgcgtgca gaccgaaacc ttgcgctcgt 11881 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca 11941 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc 12001 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg 12061 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt 12121 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat 12181 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgc 12241 gtgaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc 12301 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc 12361 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa 12421 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga 12481 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc 12541 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct 12601 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata 12661 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc 12721 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg 12781 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa 12841 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc 12901 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctctc 12961 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg 13021 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa 13081 gcgttagacg gaccgcccat gtggatcact ccgttgcccc gtcgctcacc gtgttggggg 13141 gaaggtgcac atggctcagt tctcaatgga aattatctgc ctaaccggct cagttctgcg 13201 tagaaaccaa catgcaagct ccaccgggtg caaagcggca gcggc //