LOCUS p7M35s-UZCi-Os2 13488 bp DNA circular SYN 01-APR-2026 DEFINITION p7M35s-UZCi-Os2 (H118): Ligation of C715pHCOs2* into H027p7M2x35s-UZmC9i* ACCESSION p7M35s-UZCi-Os2 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/crispr/p7M35s-UZCi-Os2 FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 174..958 /label="P2x35s'" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 976..1209 /label="Bar" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1210..1398 /label="STLS1" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" CDS 1399..1716 /label="Bar" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature 1734..2024 /label="'E9" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 2056..3564 /label="Ubi-int" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 3043..3562 /label="Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 3595..3615 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 4006..4098 /label="int" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 4204..7878 /label="Cas9WT" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 7828..7875 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 7884..8137 /label="NOS-T" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 8157..8655 /label="U6-C11-2" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" CDS 8410..8655 /label="short" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature complement(8651..8661) /label="BsmBI site (GTGT)" /note="BsmBI recognition site CGTCTC and the 4-nt overhang it leaves" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 8657..8682 /label="'targetRNA" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 8676..8686 /label="BsmBI site (GTTT)" /note="BsmBI recognition site CGTCTC and the 4-nt overhang it leaves" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 8683..8712 /label="hairpin" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 8683..8765 /label="gRNA" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 8806..9408 /label="Right Border Region" /note="color: #A6ADA2" /ApEinfo_fwdcolor="#A6ADA2" /ApEinfo_revcolor="#A6ADA2" misc_feature 8851..8874 /label="RB" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" misc_feature 9422..10080 /label="'ColE1''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature 10109..12072 /label="''pVS1 ORI'''" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" CDS 10435..11610 /label="ParB" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" CDS 12261..13271 /label="strep/spec" /note="color: #3557B7" /ApEinfo_fwdcolor="#3557B7" /ApEinfo_revcolor="#3557B7" misc_feature join(13339..13488,1..163) /label="Left Border Region" /note="color: #17281D" /ApEinfo_fwdcolor="#17281D" /ApEinfo_revcolor="#17281D" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagacg ggccaacatg 181 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa 241 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 301 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc 361 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa 421 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 481 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat 541 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 601 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 661 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 721 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 781 agaagacctt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 841 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 901 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc 961 gagtcctcca ccgaaatggg ccccgagcgc aggcccgccg acatccgccg tgccaccgag 1021 gccgacatgc cagccgtctg caccatcgtg aaccactaca tcgagacttc cacggtcaac 1081 ttccgcacag agccgcagga gcctcaggag tggaccgacg acctcgtgag gctgcgcgag 1141 cgctacccct ggctcgtcgc tgaggtggat ggcgaggtcg ccggcatcgc ctacgcgggc 1201 ccctggaagg taagtttctg cttctacctt tgatatatat ataataatta tcattaatta 1261 gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt 1321 gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca 1381 aaatttgttg atgtgcaggc acgcaacgcc tacgactgga cggcggagag caccgtctac 1441 gtgtccccac gccaccagcg caccggcctg ggctccacgc tctacaccca cctgctcaag 1501 agcctggagg cccagggctt caagtccgtg gtcgctgtga tcggcctccc caacgacccg 1561 agcgtccgta tgcacgaggc cctcggctac gcgccccgcg ggatgctgag ggccgcgggc 1621 ttcaagcacg gcaactggca cgacgtgggc ttctggcagc tcgacttctc cctgcctgtc 1681 ccaccccgcc cggtgctccc cgtcaccgaa atctgatccg gaatcgcgtt cgagtattat 1741 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt 1801 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga 1861 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt 1921 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa 1981 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc tgcaggcctt 2041 ggcggccgca ctagtctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg 2101 agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga 2161 agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct 2221 atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt 2281 ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca 2341 tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc cattttatta 2401 gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct 2461 attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat 2521 ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct 2581 ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct 2641 gttaaacgcc gtcgatcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg 2701 tcgggccaag cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag 2761 agttccgctc caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc 2821 ggcagacgtg agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac 2881 gggggattcc tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga 2941 caccccctcc acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac 3001 cagatctccc ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc 3061 cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta gggcccggta 3121 gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctgctagc 3181 gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt 3241 tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg atctaggata 3301 ggtatacatg ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta 3361 ttcatatgct ctaaccttga gtacctatct attataataa acaagtatgt tttataatta 3421 ttttgatctt gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag 3481 ccctgccttc atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg 3541 ttgtttggtg ttacttctgc aggtactaga tcctccaccg aaatggcctc cggacccaag 3601 aagaagcgca aagtgggaat ccacggagct ccagacaaga agtacagcat tggtctggac 3661 atcgggacca actccgtcgg ctgggccgtt atcaccgatg agtacaaagt gccttctaag 3721 aagttcaaag tcctcggaaa cactgacagg cacagcatca agaagaactt gattggcgcc 3781 cttctcttcg actccggcga aaccgctgag gccacccgtc tgaagcgcac cgccagaagg 3841 cgctacaccc gtcgcaagaa taggatctgc tacctccagg agatcttcag caacgagatg 3901 gccaaagtgg acgattcctt ctttcaccgt ttggaggaga gcttccttgt cgaggaggac 3961 aagaagcacg agcgccatcc catcttcgga aacattgttg acgaggtaat ttatatacaa 4021 tgacagtgca aaaaattttg atacaattaa tgcatcttaa catgtcatag ctaaaaattc 4081 tattttggtg gaatataggt ggcttaccac gagaagtatc caactatcta ccacctcaga 4141 aagaagctgg tggactccac cgacaaggcc gatttgaggc tcatctacct tgccctcgct 4201 cacatgatca agttccgcgg tcacttcctg attgagggcg acctcaaccc cgacaactct 4261 gacgtcgata agttgttcat ccaactggtc cagacctaca accagctttt cgaggagaat 4321 cctatcaacg cctccggggt tgacgccaag gctattctca gcgcccgtct gtccaagagc 4381 cgcaggcttg agaacttgat cgcccagctc ccaggagaga agaagaacgg cctcttcgga 4441 aacctgatcg ctctctcctt gggccttacc cctaacttca agtctaattt tgacctcgcc 4501 gaggacgcca agctgcaact cagcaaggac acctacgatg acgacttgga caacctgctc 4561 gcccagatcg gcgatcagta cgctgacctt ttcttggccg ccaagaacct ctccgacgct 4621 attctgctct ccgacatcct tcgcgtgaac actgagatca ccaaggcccc actgagcgcc 4681 tccatgatca agcgttacga cgaacaccac caagatttga ccctcctcaa ggctcttgtc 4741 agacagcagc tgcccgagaa gtacaaggag attttcttcg accagagcaa gaacggatac 4801 gccggttaca tcgacggcgg ggcctcccaa gaggagttct acaagttcat caagcccatc 4861 ttggagaaga tggacggcac cgaagagctg ctcgtgaagc tcaaccgcga ggatcttttg 4921 aggaagcagc gtaccttcga caacggatct attccccacc agatccatct cggcgagctg 4981 cacgctatcc tccgcaggca agaggacttc tatccattcc ttaaggacaa tcgcgagaag 5041 attgaaaaga tcctcacttt tagaatccct tactacgtcg gacccttggc ccgtggcaac 5101 agccgcttcg cctggatgac caggaagtcc gaggaaacca tcaccccatg gaacttcgag 5161 gaggtggttg acaagggggc cagcgctcag tccttcattg agcgcatgac caacttcgat 5221 aagaacctgc ccaacgagaa agtcctccca aagcactccc ttctgtacga gtacttcact 5281 gtctacaatg agttgaccaa agtgaagtac gtgaccgagg gtatgcgtaa gcctgccttc 5341 ctcagcggcg agcagaagaa ggccatcgtt gacctcctgt tcaagaccaa caggaaagtc 5401 accgtgaagc agcttaagga ggactacttc aagaagatcg agtgctttga ctctgtcgag 5461 atcagcggag tggaggatcg cttcaacgct tccttgggca cttaccacga cctcctgaag 5521 attatcaagg acaaggactt cctcgacaac gaggagaacg aagatatctt ggaggacatc 5581 gttcttaccc tcaccctgtt cgaggacaga gagatgattg aggaacgcct caagacctac 5641 gcccacttgt tcgacgataa agtcatgaag caacttaagc gtaggcgcta caccggatgg 5701 ggcaggctgt cccgtaagct catcaacggt atccgcgaca agcagagcgg caagactatt 5761 ctcgacttct tgaagtccga cggattcgcc aatcgcaact tcatgcagct gatccacgac 5821 gattctctta ccttcaagga ggacatccaa aaggctcaag tgtccggcca gggcgacagc 5881 ctccacgagc acatcgccaa cctggccggg agccccgcta ttaagaaggg aatcctccag 5941 accgtcaagg tggttgacga gttggtcaag gtgatgggca gacataagcc agagaacatc 6001 gtcatcgaga tggccaggga aaaccagacc acccagaagg gacagaagaa ctcccgtgag 6061 cgcatgaaga ggattgagga gggcatcaag gagcttggtt cccagatcct caaggagcac 6121 cccgtggaaa acacccaact ccagaatgag aagctgtatt tgtactacct tcagaacggg 6181 cgcgatatgt acgttgacca agagctggac atcaaccgcc tcagcgacta cgacgtggat 6241 cacattgtcc ctcagtcctt tctcaaggac gactctatcg acaacaaggt gttgactaga 6301 agcgataaga accgcggcaa gtccgacaac gtcccatccg aggaggtggt caagaagatg 6361 aagaattact ggaggcagct gcttaacgcc aagctcatca cccagcgcaa gttcgacaac 6421 ttgaccaagg ccgagcgtgg aggcctcagc gaactggaca aggctggatt catcaagagg 6481 caacttgttg aaacccgcca gattaccaag cacgtggccc agatcctcga ctcccgtatg 6541 aacactaagt acgatgagaa cgacaagctg atccgcgagg tcaaagtgat taccctcaag 6601 agcaagctcg tgtctgactt cagaaaggac ttccaattct acaaggttag ggagatcaac 6661 aactaccacc acgcccacga tgcttacctt aatgccgtgg tcggcaccgc cttgatcaag 6721 aagtacccca agctggagtc cgagttcgtg tatggtgact acaaggtcta cgacgttcgc 6781 aagatgatcg ctaagagcga gcaggagatt ggcaaggcca ccgccaagta cttcttctac 6841 tccaacatca tgaacttctt taagaccgag atcactttgg ctaacgggga gatccgtaag 6901 cgccctctca ttgaaaccaa cggagaaacc ggcgagatcg tgtgggacaa gggcagggac 6961 ttcgccaccg tcagaaaagt gctgagcatg ccccaagtca acatcgtgaa gaaaaccgag 7021 gttcagactg gaggcttctc caaggagtct atcctcccaa agcgcaattc cgataagttg 7081 attgcccgta agaaggactg ggaccccaag aagtacggtg gattcgacag cccaaccgtc 7141 gcctactccg tgcttgtcgt ggctaaagtt gagaagggca agagcaagaa gctcaagtcc 7201 gtcaaggagc tgctcgggat caccatcatg gagcgcagct ccttcgagaa gaaccctatt 7261 gatttccttg aggccaaggg ctacaaggaa gtgaagaagg acttgatcat caagctcccc 7321 aagtactctc tgttcgagct tgagaacgga aggaagcgta tgctcgcctc cgctggcgag 7381 ctgcaaaagg gaaacgagtt ggccctccca agcaagtacg tcaacttcct gtacctcgcc 7441 tcccactatg agaagctcaa gggcagcccc gaggacaacg aacagaagca gttgtttgtg 7501 gagcagcata agcactacct tgacgagatc attgagcaga tcagcgagtt ctccaagcgc 7561 gttatcctgg ctgacgccaa tctcgataaa gtcctttctg cctacaacaa gcacagggac 7621 aagcctatca gagagcaggc tgaaaacatt atccacttgt tcaccctcac caacctgggt 7681 gccccagccg ccttcaagta cttcgacact accatcgacc gcaagcgtta cacctccacc 7741 aaggaagtgc tcgatgctac ccttatccac cagagcatta ctgggttgta cgaaaccagg 7801 atcgacctgt cccaactcgg cggagacaag cgccccgccg ccaccaagaa ggctggccag 7861 gccaagaaga agaagtaacc cgggatcgtt caaacatttg gcaataaagt ttcttaagat 7921 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc 7981 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag 8041 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata 8101 aattatcgcg cgcggtgtca tctatgttac tagatcgctc gaattcgacc tcgagcatct 8161 gtagtagcac agattaattc agcaaatgaa accaaaaaaa aaaagagagt tactcagagt 8221 aagccttgtc atctactatg taattataag caagcataaa tcataattca tgtgttattg 8281 atcattttta tggagtaatc tgggtgtgaa actgcgaata atttatcagt attattatgg 8341 gtgctttaca cgtttcgatg atgcgcttgc atcgtgagac cgtgaagagg tagatgtcga 8401 tctgccgccg gatcatgaac caacggcctg gctgtatttg gtggttgtgt agggagatgg 8461 ggagaagaaa agcccgattc tcttcgctgt gatgggctgg atgcatgcgg gggagcggga 8521 ggcccaagta cgtgcacggt gagcggccca cagggcgagt gtgagcgcga gaggcgggag 8581 gaacagttta gtaccacatt gcccagctaa ctcgaacgcg accaacttat aaacccgcgc 8641 gctgtcgctt gtgttgagac gttcgaagct tctgtcgtct cagttttaga gctagaaata 8701 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 8761 tttttgtcga caggccttaa gggccagatc ttgggcccgg tacccgatca gattgtcgtt 8821 tcccgccttc ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga 8881 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt 8941 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggagtgctt ggcattccgt 9001 gcgataatga cttctgttca accacccaaa cgtcggaaag cctgacgacg gagcagcatt 9061 ccaaaaagat cccttggctc gtctgggtcg gctagaaggt cgagtgggct gctgtggctt 9121 gatccctcaa cgcggtcgcg gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg 9181 acgctgtcga aaagcgtgat ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc 9241 acgcgccaaa gttccgtcac aggatgatct ggcgcgagtt gctggatctc gccttcaatc 9301 cgggtctgtg gcgggaactc cacgaaaata tccgaacgca gcaagatatc gcggtgcatc 9361 tcggtcttgc ctgggcagtc gccgccgacg ccgttgatgt ggacgccggc ttagctcatg 9421 gccaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 9481 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 9541 ccatcgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 9601 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 9661 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 9721 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 9781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgctcaca gcccagcttg 9841 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 9901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 9961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 10021 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 10081 aacgccagca acgcggcctt tcctaagggt taatgaggta aagagaaaat gagcaaaagc 10141 acaaacacgc taagtgccgg tcgtccgagc gcacgcagca gcaaggctgc aacgttggcc 10201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac 10261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa 10321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc 10381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc 10441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg 10501 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc 10561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggcggcgcag 10621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagccgcc 10681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg 10741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc 10801 acccgcgata gtcgcagcat catggacgtg gcagtattcc gcctctcgaa acgtgatcgc 10861 cgggcgggcg aagtcatccg ctacgaactg ccggacggtc atgtcgaggt gagcgcaggc 10921 ccggcgggca tggcctccgt ctgggattat gacctcgttc tgatggccgt gtcgcacctt 10981 acggaaagca tgaatcgcta ccgggagggc aagggtgata agcccggtcg cgtattccgt 11041 ccgcatgtgg ccgacgttct gaaattctgt cgccgggcgg atggcggcaa gcaaaaggac 11101 gatctcgtcg aaacctgcat ccgcctgaac acgacccacg tcgccatgca gcgcaccaag 11161 aaagcgaaga atggccgtct tgtgactgtc tccgagggcg aagccctgat ctcgcgctac 11221 aagatcgtaa agagcgaaac cgggcggccc gagtacatcg agatcgagct tgctgattgg 11281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac 11341 tttttgattg atcccggcat cggtcgtttt ctctaccgcc tggcacgccg cgccgcaggc 11401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag 11461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac 11521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg 11581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc 11641 ctagcagggg aaaaaggtcg aaaagctctc tttcctgtgg atagcacgta cattgggaac 11701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg 11761 aaccgggcac gcatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa 11821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc 11881 cagcgcacag ccgaatagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc 11941 cccgccgctt cgcgtcggcc tatcgtggcc gctggccgct caaaaatggc tggcctacgg 12001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccgccgccca 12061 catcaaggca cctcagcggt gggtatgcct gacgatgcgt gcagaccgaa accttgcgct 12121 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac 12181 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta 12241 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca 12301 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac 12361 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt 12421 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca 12481 tgcgtgaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 12541 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 12601 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 12661 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 12721 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 12781 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 12841 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 12901 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 12961 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 13021 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 13081 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 13141 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct 13201 ctcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag 13261 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact 13321 caagcgttag acggaccgcc catgtggatc actccgttgc cccgtcgctc accgtgttgg 13381 ggggaaggtg cacatggctc agttctcaat ggaaattatc tgcctaaccg gctcagttct 13441 gcgtagaaac caacatgcaa gctccaccgg gtgcaaagcg gcagcggc //