LOCUS H048p9D35s-C9wt-U6Ats 13018 bp DNA circular SYN 21-JAN-2026 DEFINITION Ligation of G873pHC-U6Ats-XmaKpn* into E733p9D35s-C9wt-U6GmNhe* ACCESSION H048p9D35s-C9wt-U6Ats KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct FEATURES Location/Qualifiers misc_feature 1..24 /label="LB'" /SECDrawAs="Gene" misc_feature 169..584 /label="35S" /SECDrawAs="Region" CDS 605..901 /label="NptIIo" /SECDrawAs="Gene" CDS 902..1090 /label="'intron" /SECDrawAs="Gene" CDS 1091..1594 /label="NptIIo" /SECDrawAs="Gene" misc_feature 1608..1898 /label="'E9" /SECDrawAs="Region" misc_feature 1924..3399 /label="Pro-Ubq10At" /SECDrawAs="Region" CDS 3028..3091 /label="Ubi cds 5`" /SECDrawAs="Gene" misc_feature 3094..3397 /label="íntron" /SECDrawAs="Region" CDS 3421..7617 /label="'Cas9WT" /SECDrawAs="Gene" misc_feature 3427..3447 /label="NLS" /SECDrawAs="Region" misc_feature 7567..7614 /label="NLS" /SECDrawAs="Region" misc_feature 7623..7876 /label="NOS-T" /SECDrawAs="Region" CDS 7886..8185 /label="'U6Ats" /SECDrawAs="Gene" CDS 8186..8212 /label="targetRNA" /SECDrawAs="Gene" CDS 8213..8242 /label="hairpin" /SECDrawAs="Gene" misc_feature 8213..8295 /label="gRNA" /SECDrawAs="Region" misc_feature 8336..8938 /label="Right Border Region" /SECDrawAs="Region" misc_feature 8381..8404 /label="RB" /SECDrawAs="Gene" misc_feature 8952..9610 /label="'ColE1''" /SECDrawAs="Region" misc_feature 9639..11602 /label="''pVS1 ORI'''" /SECDrawAs="Region" CDS 9965..11140 /label="ParB" /SECDrawAs="Gene" CDS 11791..12801 /label="strep/spec" /SECDrawAs="Gene" misc_feature join(12869..13018,1..163) /label="Left Border Region" /SECDrawAs="Region" ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc 181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat 601 aactatggga gcatggattg agcaggatgg cctccatgct ggtagccccg ctgcttgggt 661 ggagcgcctc tttggctacg actgggccca acagaccatt gggtgcagtg atgccgctgt 721 gttccgtttg agcgcccagg gcaggccagt tctcttcgtc aagactgacc tctccggtgc 781 cctcaacgag ttgcaggacg aagctgcccg cctctcatgg cttgccacca ccggagtccc 841 ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga gactggctgc tcttgggcga 901 ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat 961 ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct 1021 gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt 1081 tgatgtgcag gtgccaggcc aagacctcct gagctcccac ctggccccag ccgagaaggt 1141 gtccatcatg gcagacgcca tgcgtcgcct ccacaccctg gacccagcca cctgcccctt 1201 cgaccaccag gccaagcaca ggatcgaacg tgcacgcact aggatggagg ccggccttgt 1261 ggaccaggat gaccttgacg aggagcacca gggccttgct cccgccgagc tgttcgcccg 1321 cctcaaggct agaatgcctg acggagagga cttggtggtc acccacgggg atgcctgcct 1381 ccccaacatc atggttgaga acggacgctt ctctggcttc atcgactgcg gtcgtctggg 1441 cgtggccgac cgctaccaag acatcgccct ggccaccagg gacatcgctg aggaacttgg 1501 aggggagtgg gccgatcgtt tcttggtcct ctacggcatt gctgcccccg actcccagag 1561 gatcgccttc taccgcctgc ttgacgagtt cttctgaacg cgttcgagta ttatggcatt 1621 gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg 1681 gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt 1741 ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa 1801 tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg 1861 cctctaatga ccgaagttaa tatgaggagt aaaacactga agcctgcagg ccttggcggc 1921 cgctttcctt acattctgag cctctttcct tctaatccac tcatctgcat cttcttgtgt 1981 ccttactaat acctcattgg ttccaaattc cctcccttta agcaccagct cgtttctgtt 2041 cttccacagc ctcccaagta tccaagggac taaagcctcc acattcttca gatcaggata 2101 ttcttgttta agatgttgaa ctctatggag gtttgtatga actgatgatc taggaccgga 2161 taagttccct tcttcatagc gaacttattc aaagaatgtt ttgtgtatca ttcttgttac 2221 attgttatta atgaaaaaat attattggtc attggactga acacgagtgt taaatatgga 2281 ccaggcccca aataagatcc attgatatat gaattaaata acaagaataa atcgagtcac 2341 caaaccactt gcctttttta acgagacttg ttcaccaact tgatacaaaa gtcattatcc 2401 tatgcaaatc aataatcata caaaaatatc caataacact aaaaaattaa aagaaatgga 2461 taatttcaca atatgttata cgataaagaa gttacttttc caagaaattc actgatttta 2521 taagcccact tgcattagat aaatggcaaa aaaaaacaaa aaggaaaaga aataaagcac 2581 gaagaattct agaaaatacg aaatacgctt caatgcagtg ggacccacgg ttcaattatt 2641 gccaattttc agctccaccg tatatttaaa aaataaaacg ataatgctaa aaaaatataa 2701 atcgtaacga tcgttaaatc tcaacggctg gatcttatga cgaccgttag aaattgtggt 2761 tgtcgacgag tcagtaataa acggcgtcaa agtggttgca gccggcacac acgagtcgtg 2821 tttatcaact caaagcacaa atacttttcc tcaacctaaa aataaggcaa ttagccaaaa 2881 acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt ttattattag ctattgcttc 2941 accgccttag ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt cttctataaa 3001 acaataccca aagagctctt cttcttcaca attcagattt caatttctca aaatcttaaa 3061 aactttctct caattctctc taccgtgatc aaggtaaatt tctgtgttcc ttattctctc 3121 aaaatcttcg attttgtttt cgttcgatcc caatttcgta tatgttcttt ggtttagatt 3181 ctgttaatct tagatcgaag acgattttct gggtttgatc gttagatatc atcttaattc 3241 tcgattaggg tttcatagat atcatccgat ttgttcaaat aatttgagtt ttgtcgaata 3301 attactcttc gatttgtgat ttctatctag atctggtgtt agtttctagt ttgtgcgatc 3361 gaatttgtcg attaatctga gtttttctga ttaacaggta ctagtggatc ctccataact 3421 atggccccca agaagaagcg caaagtggga atccacggag ctccagacaa gaagtacagc 3481 attggtctgg acatcgggac caactccgtc ggctgggccg ttatcaccga tgagtacaaa 3541 gtgccttcta agaagttcaa agtcctcgga aacactgaca ggcacagcat caagaagaac 3601 ttgattggcg cccttctctt cgactccggc gaaaccgctg aggccacccg tctgaagcgc 3661 accgccagaa ggcgctacac ccgtcgcaag aataggatct gctacctcca ggagatcttc 3721 agcaacgaga tggccaaagt ggacgattcc ttctttcacc gtttggagga gagcttcctt 3781 gtcgaggagg acaagaagca cgagcgccat cccatcttcg gaaacattgt tgacgaggtg 3841 gcttaccacg agaagtatcc aactatctac cacctcagaa agaagctggt ggactccacc 3901 gacaaggccg atttgaggct catctacctt gccctcgctc acatgatcaa gttccgcggt 3961 cacttcctga ttgagggcga cctcaacccc gacaactctg acgtcgataa gttgttcatc 4021 caactggtcc agacctacaa ccagcttttc gaggagaatc ctatcaacgc ctccggggtt 4081 gacgccaagg ctattctcag cgcccgtctg tccaagagcc gcaggcttga gaacttgatc 4141 gcccagctcc caggagagaa gaagaacggc ctcttcggaa acctgatcgc tctctccttg 4201 ggccttaccc ctaacttcaa gtctaatttt gacctcgccg aggacgccaa gctgcaactc 4261 agcaaggaca cctacgatga cgacttggac aacctgctcg cccagatcgg cgatcagtac 4321 gctgaccttt tcttggccgc caagaacctc tccgacgcta ttctgctctc cgacatcctt 4381 cgcgtgaaca ctgagatcac caaggcccca ctgagcgcct ccatgatcaa gcgttacgac 4441 gaacaccacc aagatttgac cctcctcaag gctcttgtca gacagcagct gcccgagaag 4501 tacaaggaga ttttcttcga ccagagcaag aacggatacg ccggttacat cgacggcggg 4561 gcctcccaag aggagttcta caagttcatc aagcccatct tggagaagat ggacggcacc 4621 gaagagctgc tcgtgaagct caaccgcgag gatcttttga ggaagcagcg taccttcgac 4681 aacggatcta ttccccacca gatccatctc ggcgagctgc acgctatcct ccgcaggcaa 4741 gaggacttct atccattcct taaggacaat cgcgagaaga ttgaaaagat cctcactttt 4801 agaatccctt actacgtcgg acccttggcc cgtggcaaca gccgcttcgc ctggatgacc 4861 aggaagtccg aggaaaccat caccccatgg aacttcgagg aggtggttga caagggggcc 4921 agcgctcagt ccttcattga gcgcatgacc aacttcgata agaacctgcc caacgagaaa 4981 gtcctcccaa agcactccct tctgtacgag tacttcactg tctacaatga gttgaccaaa 5041 gtgaagtacg tgaccgaggg tatgcgtaag cctgccttcc tcagcggcga gcagaagaag 5101 gccatcgttg acctcctgtt caagaccaac aggaaagtca ccgtgaagca gcttaaggag 5161 gactacttca agaagatcga gtgctttgac tctgtcgaga tcagcggagt ggaggatcgc 5221 ttcaacgctt ccttgggcac ttaccacgac ctcctgaaga ttatcaagga caaggacttc 5281 ctcgacaacg aggagaacga agatatcttg gaggacatcg ttcttaccct caccctgttc 5341 gaggacagag agatgattga ggaacgcctc aagacctacg cccacttgtt cgacgataaa 5401 gtcatgaagc aacttaagcg taggcgctac accggatggg gcaggctgtc ccgtaagctc 5461 atcaacggta tccgcgacaa gcagagcggc aagactattc tcgacttctt gaagtccgac 5521 ggattcgcca atcgcaactt catgcagctg atccacgacg attctcttac cttcaaggag 5581 gacatccaaa aggctcaagt gtccggccag ggcgacagcc tccacgagca catcgccaac 5641 ctggccggga gccccgctat taagaaggga atcctccaga ccgtcaaggt ggttgacgag 5701 ttggtcaagg tgatgggcag acataagcca gagaacatcg tcatcgagat ggccagggaa 5761 aaccagacca cccagaaggg acagaagaac tcccgtgagc gcatgaagag gattgaggag 5821 ggcatcaagg agcttggttc ccagatcctc aaggagcacc ccgtggaaaa cacccaactc 5881 cagaatgaga agctgtattt gtactacctt cagaacgggc gcgatatgta cgttgaccaa 5941 gagctggaca tcaaccgcct cagcgactac gacgtggatc acattgtccc tcagtccttt 6001 ctcaaggacg actctatcga caacaaggtg ttgactagaa gcgataagaa ccgcggcaag 6061 tccgacaacg tcccatccga ggaggtggtc aagaagatga agaattactg gaggcagctg 6121 cttaacgcca agctcatcac ccagcgcaag ttcgacaact tgaccaaggc cgagcgtgga 6181 ggcctcagcg aactggacaa ggctggattc atcaagaggc aacttgttga aacccgccag 6241 attaccaagc acgtggccca gatcctcgac tcccgtatga acactaagta cgatgagaac 6301 gacaagctga tccgcgaggt caaagtgatt accctcaaga gcaagctcgt gtctgacttc 6361 agaaaggact tccaattcta caaggttagg gagatcaaca actaccacca cgcccacgat 6421 gcttacctta atgccgtggt cggcaccgcc ttgatcaaga agtaccccaa gctggagtcc 6481 gagttcgtgt atggtgacta caaggtctac gacgttcgca agatgatcgc taagagcgag 6541 caggagattg gcaaggccac cgccaagtac ttcttctact ccaacatcat gaacttcttt 6601 aagaccgaga tcactttggc taacggggag atccgtaagc gccctctcat tgaaaccaac 6661 ggagaaaccg gcgagatcgt gtgggacaag ggcagggact tcgccaccgt cagaaaagtg 6721 ctgagcatgc cccaagtcaa catcgtgaag aaaaccgagg ttcagactgg aggcttctcc 6781 aaggagtcta tcctcccaaa gcgcaattcc gataagttga ttgcccgtaa gaaggactgg 6841 gaccccaaga agtacggtgg attcgacagc ccaaccgtcg cctactccgt gcttgtcgtg 6901 gctaaagttg agaagggcaa gagcaagaag ctcaagtccg tcaaggagct gctcgggatc 6961 accatcatgg agcgcagctc cttcgagaag aaccctattg atttccttga ggccaagggc 7021 tacaaggaag tgaagaagga cttgatcatc aagctcccca agtactctct gttcgagctt 7081 gagaacggaa ggaagcgtat gctcgcctcc gctggcgagc tgcaaaaggg aaacgagttg 7141 gccctcccaa gcaagtacgt caacttcctg tacctcgcct cccactatga gaagctcaag 7201 ggcagccccg aggacaacga acagaagcag ttgtttgtgg agcagcataa gcactacctt 7261 gacgagatca ttgagcagat cagcgagttc tccaagcgcg ttatcctggc tgacgccaat 7321 ctcgataaag tcctttctgc ctacaacaag cacagggaca agcctatcag agagcaggct 7381 gaaaacatta tccacttgtt caccctcacc aacctgggtg ccccagccgc cttcaagtac 7441 ttcgacacta ccatcgaccg caagcgttac acctccacca aggaagtgct cgatgctacc 7501 cttatccacc agagcattac tgggttgtac gaaaccagga tcgacctgtc ccaactcggc 7561 ggagacaagc gccccgccgc caccaagaag gctggccagg ccaagaagaa gaagtaaccc 7621 gggatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg 7681 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat 7741 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat 7801 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat 7861 ctatgttact agatcgctcg aattcaactt tccattcgga gtttttgtat cttgtttcat 7921 agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga ttgaataaaa 7981 catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag 8041 agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag 8101 ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata 8161 tacagctaga gtcgaagtag tgattgagac gttcgaagct tctgtcgtct cagttttaga 8221 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga 8281 gtcggtgctt tttttgtcga caggccttaa gggccagatc ttgggcccgg tacccgatca 8341 gattgtcgtt tcccgccttc ggtttaaact atcagtgttt gacaggatat attggcgggt 8401 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg 8461 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggagtgctt 8521 ggcattccgt gcgataatga cttctgttca accacccaaa cgtcggaaag cctgacgacg 8581 gagcagcatt ccaaaaagat cccttggctc gtctgggtcg gctagaaggt cgagtgggct 8641 gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc agcgccgaaa aatcctcgat 8701 cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg ctctttcggc cgacgtcctg 8761 gccagtcatc acgcgccaaa gttccgtcac aggatgatct ggcgcgagtt gctggatctc 8821 gccttcaatc cgggtctgtg gcgggaactc cacgaaaata tccgaacgca gcaagatatc 8881 gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg ccgttgatgt ggacgccggc 8941 ttagctcatg gccaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 9001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 9061 aacaaaaaaa ccatcgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 9121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 9181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 9241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 9301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgctcaca 9361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 9421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 9481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 9541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 9601 cctatggaaa aacgccagca acgcggcctt tcctaagggt taatgaggta aagagaaaat 9661 gagcaaaagc acaaacacgc taagtgccgg tcgtccgagc gcacgcagca gcaaggctgc 9721 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc 9781 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct 9841 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat 9901 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg 9961 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct 10021 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa 10081 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 10141 ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 10201 gcaagccgcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 10261 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 10321 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gcagtattcc gcctctcgaa 10381 acgtgatcgc cgggcgggcg aagtcatccg ctacgaactg ccggacggtc atgtcgaggt 10441 gagcgcaggc ccggcgggca tggcctccgt ctgggattat gacctcgttc tgatggccgt 10501 gtcgcacctt acggaaagca tgaatcgcta ccgggagggc aagggtgata agcccggtcg 10561 cgtattccgt ccgcatgtgg ccgacgttct gaaattctgt cgccgggcgg atggcggcaa 10621 gcaaaaggac gatctcgtcg aaacctgcat ccgcctgaac acgacccacg tcgccatgca 10681 gcgcaccaag aaagcgaaga atggccgtct tgtgactgtc tccgagggcg aagccctgat 10741 ctcgcgctac aagatcgtaa agagcgaaac cgggcggccc gagtacatcg agatcgagct 10801 tgctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 10861 ccccgattac tttttgattg atcccggcat cggtcgtttt ctctaccgcc tggcacgccg 10921 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 10981 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 11041 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 11101 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 11161 gcaaattgcc ctagcagggg aaaaaggtcg aaaagctctc tttcctgtgg atagcacgta 11221 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 11281 gtacattggg aaccgggcac gcatgtaagt gactgatata aaagagaaaa aaggcgattt 11341 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 11401 actgtctggc cagcgcacag ccgaatagct gcaaaaagcg cctacccttc ggtcgctgcg 11461 ctccctacgc cccgccgctt cgcgtcggcc tatcgtggcc gctggccgct caaaaatggc 11521 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 11581 ccgccgccca catcaaggca cctcagcggt gggtatgcct gacgatgcgt gcagaccgaa 11641 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt 11701 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt 11761 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg 11821 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt 11881 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga 11941 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag 12001 ttaaacatca tgcgtgaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt 12061 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca 12121 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg 12181 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct 12241 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt 12301 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt 12361 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa 12421 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt 12481 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc 12541 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca 12601 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg 12661 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat 12721 cgcttggcct ctcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc 12781 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg 12841 cggcttaact caagcgttag acggaccgcc catgtggatc actccgttgc cccgtcgctc 12901 accgtgttgg ggggaaggtg cacatggctc agttctcaat ggaaattatc tgcctaaccg 12961 gctcagttct gcgtagaaac caacatgcaa gctccaccgg gtgcaaagcg gcagcggc //