LOCUS pU6pp-LbCpf1 7597 bp DNA circular SYN 14-NOV-2018 DEFINITION pU6pp-LbCpf1 (D054): Ligation of TargetCpf1_EcoSal* into D042pUbiZmU6pp-Cpf1* ACCESSION pU6pp-LbCpf1 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/crispr/pU6pp-LbCpf1 FEATURES Location/Qualifiers misc_feature 98..1606 /label="Ubi-int" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 1085..1604 /label="Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 1625..5407 /label="ORF-1" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 1631..1651 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 5357..5404 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 5413..5666 /label="NOS-T" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 5676..5994 /label="U6 Pp" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 5996..6016 /label="DR" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature complement(6095..6759) /label="ColE1" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature complement(join(6760..7597,1..25)) /label="Amp" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" ORIGIN 1 cgacacggaa atgttgaata ctcatggctg acctcctatg cagatatctt atgagtgact 61 agaatagctc tcaagatcag gccttggcgg ccctagtctg cagtgcagcg tgacccggtc 121 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat 181 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt 241 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata 301 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac 361 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata 421 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata 481 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa 541 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga 601 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt 661 ttcgagtaga taatgccagc ctgttaaacg ccgtcgatcg acgagtctaa cggacaccaa 721 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 781 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 841 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 901 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 961 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 1021 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 1081 caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc 1141 ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt 1201 gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc 1261 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg 1321 cagacgggat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac 1381 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat 1441 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc 1501 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt 1561 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcaggtactg gatcctccat 1621 agcaatggct cccaagaaga agcgcaaggt gggcatccac ggagtcccag ccgcctccaa 1681 gctggagaag ttcaccaact gctacagcct ctctaaaacc ctccgtttca aggcaattcc 1741 tgttggtaag actcaggaga acatcgacaa caagaggctt ctcgtggagg acgaaaagag 1801 agccgaggat tacaagggcg tcaagaagct gctcgaccgc tactacttgt ccttcatcaa 1861 tgacgtgctt cactcgatca agctcaagaa cctgaacaac tacattagcc tcttccgtaa 1921 gaaaaccagg acagagaagg agaacaagga gttggaaaac cttgagatca atctccgcaa 1981 agagatcgcc aaggctttta aggggaacga gggctataag tccctgttca agaaggacat 2041 cattgagact atcctaccgg agttcctcga cgataaggac gaaatcgcct tggtcaactc 2101 attcaacgga ttcaccacgg cgttcaccgg cttcttcgac aaccgggaga atatgttcag 2161 cgaggaggcc aagtccacca gtatcgcttt tcgctgcatt aacgagaacc tgacccgtta 2221 catctccaac atggacatct tcgaaaaggt tgatgccatc ttcgacaagc acgaggtgca 2281 agagattaag gagaagatcc tcaacagcga ctacgacgtg gaggacttct tcgaaggaga 2341 gttcttcaac ttcgtcctta cccaggaggg catcgatgtc tacaacgcca ttatcggtgg 2401 cttcgttacc gagtctggag agaagatcaa ggggctcaat gagtacatca acctctacaa 2461 ccagaaaacc aagcagaagc tgcccaagtt taagccactt tacaagcaag tgctttccga 2521 cagggaaagc ctgtcgttct acggcgaggg ctacacatcc gacgaggagg tcttggaggt 2581 gttccgcaac accctcaaca agaacagcga aattttctcc tctatcaaga agctggagaa 2641 gctcttcaag aacttcgacg agtactcaag cgctggaatc ttcgtcaaga atggtcccgc 2701 catctccact atttccaagg acatcttcgg cgagtggaac gttatcagag ataagtggaa 2761 cgcagagtat gacgacatcc atcttaagaa gaaagccgtg gtcaccgaga agtacgagga 2821 cgatcgccgt aagagcttca agaagattgg atcgttttcc ttggaacagc tccaggagta 2881 cgccgacgct gacctgtctg tggtcgagaa gctcaaggag atcatcatac agaaggtaga 2941 cgaaatctac aaggtgtacg gcagctccga gaagttgttc gacgccgatt tcgtccttga 3001 gaagagcctc aagaagaacg acgcggttgt ggccattatg aaagacctgc tcgactccgt 3061 caagtcattc gagaactaca tcaaggcttt cttcggggag ggcaaggaga ctaacaggga 3121 cgaatcgttc tacggagatt tcgtgcttgc ctacgacatc ttgctcaagg ttgaccacat 3181 ctacgacgcc attcgcaatt acgtcaccca aaagccttat tccaaggata agttcaagct 3241 gtactttcag aacccgcagt tcatgggcgg ttgggacaag gacaaggaga ctgactaccg 3301 agctaccatc ctccgttacg gcagcaagta ctaccttgcc atcatggaca agaaatacgc 3361 aaagtgcctg caaaagatcg ataaggacga cgtgaacgga aactacgaga agattaacta 3421 caagttgctc ccaggcccca acaagatgct gcctaaggtg ttcttctcta agaagtggat 3481 ggcctactac aatccctccg aggacatcca gaagatttat aagaacggga ccttcaagaa 3541 aggagatatg ttcaacctca acgactgcca caagttgatc gacttcttca aggacagcat 3601 ctcccgctac ccaaagtgga gtaacgccta cgacttcaac tttagcgaga cagaaaagta 3661 caaggatatc gctggcttct acagggaggt cgaggagcag ggttacaagg tcagcttcga 3721 gtctgcctcg aagaaggagg ttgacaagct ggtggaagag ggcaagctct atatgttcca 3781 gatttacaac aaggacttct ccgacaagtc ccacggaacc ccgaatcttc acaccatgta 3841 cttcaagctc ttgttcgacg agaacaacca cggccaaatc agactttcag gcggggcgga 3901 gctcttcatg cgccgtgcct ccctgaagaa agaggaactc gtcgttcacc ccgctaacag 3961 cccaatcgcc aacaagaacc ctgataatcc caagaaaacc accacgttgt cctacgacgt 4021 gtataaggac aagaggttca gcgaggacca gtacgagctg cacatcccaa tcgccattaa 4081 caagtgcccc aagaacatct ttaagatcaa caccgaggtc cgcgtgcttc tcaagcatga 4141 cgataaccct tacgtcatcg gaattgaccg tggcgagagg aacctactct acatcgtggt 4201 cgtggacggt aagggaaata tcgttgagca gtactctttg aacgaaatca ttaacaactt 4261 caacggcatc cgcatcaaga cggactacca ctccctgctc gataagaaag agaaggagag 4321 attcgaggca cgccagaact ggacctcgat cgagaatatt aaggaactta aggctggcta 4381 catcagccaa gtcgtccaca agatttgcga gctggtggag aagtacgacg ccgtgatcgc 4441 cttggaggac ctcaactccg ggttcaagaa ctcacgggtt aaggtcgaga agcaggtgta 4501 ccagaagttc gagaagatgc tcatcgacaa gctgaactac atggtggaca agaaatccaa 4561 cccgtgcgcc accggaggcg ctcttaaggg ttaccaaatc accaacaagt tcgaatcttt 4621 caagagcatg tccactcaga acggcttcat cttctacatt cccgcctggc tcaccagcaa 4681 gatcgatcca tccaccggat ttgtcaattt gctcaagaca aagtacacct cgatcgcgga 4741 cagcaagaag ttcatctcca gtttcgaccg tattatgtac gttcccgagg aggacctgtt 4801 cgagttcgcc cttgactaca agaacttctc taggaccgat gctgactaca tcaagaagtg 4861 gaagttgtac tcctacggca accgcatccg catcttccgt aacccgaaga agaacaacgt 4921 gttcgactgg gaggaagtct gcctcacgag cgcctacaaa gagctgttca ataagtacgg 4981 aattaactat cagcagggcg acatcagggc cttgctttgc gagcaatccg ataaggcatt 5041 ttacagctca ttcatggccc tcatgtccct catgctccag atgcgcaaca gcatcaccgg 5101 gagaactgac gtggacttcc tgatctcgcc tgtcaagaac tccgacggca ttttctacga 5161 ctctcgtaac tacgaggctc aggagaacgc catccttcca aagaatgccg atgctaacgg 5221 tgcctacaac atcgcgagga aggttttgtg ggccatcgga cagttcaaga aggctgagga 5281 cgaaaagctg gacaaggtca agattgccat cagcaacaag gagtggttgg agtacgccca 5341 aacctccgtg aagcacaaac gccccgcagc caccaagaag gctggccagg ccaagaagaa 5401 gaagtaaccc gggatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt 5461 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt 5521 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta 5581 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc 5641 gcggtgtcat ctatgttact agatcgctcg aattcattga atgtccattg aagcagacgt 5701 gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt gtggttcttc 5761 cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg cagcgggaga 5821 gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg ccgagtggac 5881 gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata taacccacat 5941 ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac aaccgtaatt 6001 tctactaagt gtagatgaga cgttcgaagc ttctgtcgtc tcagtcgaca ggccttaagg 6061 gcctgatctg tataaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6721 agtggaacga aaactcacgt taagggattt tggccatgag atcaccaatg cttaatcagt 6781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6901 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 7021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgct 7081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 7141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 7201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 7261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 7321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca 7381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 7441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 7501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 7561 acaggaaggc aaaatgccgc aaaaaaggga ataaggg //