LOCUS pU6pp-Pb2Cpf1 7663 bp DNA circular SYN 07-JUN-2019 DEFINITION pU6pp-Pb2Cpf1 (D521): Ligation of Pb2Cpf1-Bam-Xma* into D054pU6pp-LbCpf1* ACCESSION pU6pp-Pb2Cpf1 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/crispr/pU6pp-Pb2Cpf1 FEATURES Location/Qualifiers misc_feature 98..1606 /label="Ubi-int" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 1085..1604 /label="Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 1625..5473 /label="ORF-1" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 1631..1651 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 5423..5470 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 5479..5732 /label="NOS-T" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 5742..6060 /label="U6 Pp" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 6062..6082 /label="DR" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature complement(6161..6825) /label="ColE1" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature complement(join(6826..7663,1..25)) /label="Amp" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" ORIGIN 1 cgacacggaa atgttgaata ctcatggctg acctcctatg cagatatctt atgagtgact 61 agaatagctc tcaagatcag gccttggcgg ccctagtctg cagtgcagcg tgacccggtc 121 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat 181 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt 241 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata 301 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac 361 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata 421 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata 481 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa 541 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga 601 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt 661 ttcgagtaga taatgccagc ctgttaaacg ccgtcgatcg acgagtctaa cggacaccaa 721 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 781 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 841 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 901 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 961 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 1021 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 1081 caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc 1141 ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt 1201 gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc 1261 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg 1321 cagacgggat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac 1381 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat 1441 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc 1501 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt 1561 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcaggtactg gatcctccat 1621 agcaatggct cccaagaaga agcgcaaagt gatgcagatc aacaacctca agattatcta 1681 catgaagttc accgacttca ctggcctgta ctccttgagc aagacccttc gtttcgagtt 1741 gaagccaatc ggaaagaccc tggagaatat caagaaggcc ggtctgctcg aacaagacca 1801 gcacagggcc gattcttaca agaaagtcaa gaagattatc gacgagtacc acaaggcctt 1861 catcgagaag tccctttcga actttgagct gaagtaccag agcgaggaca agctcgactc 1921 cttggaggaa tatctcatgt actacagcat gaagcgcatc gagaaaacag agaaggataa 1981 gttcgcaaag attcaggaca accttagaaa gcaaatcgcc gaccacctca agggcgacga 2041 gtcatacaag accatcttct ccaaggacct catcaggaag aacctccctg atttcgttaa 2101 gagtgacgag gagcgcaccc tgattaagga gttcaaggac ttcacgacct acttcaaggg 2161 gttctacgaa aaccgtgaga acatgtactc cgctgaggac aagagcaccg ccatctctca 2221 ccggatcatt catgagaatt tgccgaagtt cgtggacaac atcaacgcct tttccaagat 2281 catccttatt cccgaactcc gcgagaagct gaaccagatc taccaggatt tcgaggagta 2341 cctcaacgtc gagagcatcg acgagatctt ccacttggac tacttctcga tggtgatgac 2401 tcaaaagcag attgaagtct acaacgcgat catcggcgga aagtccacca atgacaagaa 2461 gatccagggc cttaacgagt acattaacct ctacaaccag aagcacaagg attgcaagct 2521 gccaaagcta aagctcttgt tcaagcaaat cctgagcgac aggatcgcta tttcatggct 2581 ccccgacaac ttcaaggacg accaggaggc ccttgattcc atcgacacct gctacaagaa 2641 cctcctgaat gacggaaacg ttttgggcga gggtaacctc aaacttctgt tggagaacat 2701 cgacacttac aacctcaagg gcatcttcat tcgtaacgat ctccagctta ccgacatctc 2761 tcagaagatg tatgccagct ggaatgtgat ccaagacgct gtcatactgg acctcaagaa 2821 gcaagtgtcc cgcaagaaga aggagtcggc cgaagattac aacgacagat tgaagaagct 2881 ctacacctcc caggagagct tctccatcca gtacctgaac gactgcctta gggcctacgg 2941 aaagaccgag aacatccagg actacttcgc aaagctcggg gccgtcaaca acgagcacga 3001 gcagacaatt aatctgttcg ctcaagtgcg caacgcctac accagcgttc aggcgatctt 3061 gacgacccct tacccggaaa acgccaacct cgctcaggac aaggagactg tcgcccttat 3121 caagaacctg ctcgactctt tgaaacgtct gcagcgcttt attaagccac tccttggcaa 3181 gggagatgag tccgacaagg acgagcgatt ctatggcgac ttcactcccc tctgggagac 3241 attgaaccaa atcacccctc tctacaatat ggtgaggaac tacatgaccc gcaagccgta 3301 ctcacaggag aagatcaagc tgaacttcga aaacagcacc cttctcggtg gctgggattt 3361 gaacaaggag cacgacaaca ccgccatcat tctgcgtaag aatggcctct actaccttgc 3421 tatcatgaag aagtccgcca acaagatctt cgacaaggac aagctcgaca actcgggaga 3481 ttgctacgag aagatggtct acaaactgtt gcccggcgcc aacaagatgc tcccaaaagt 3541 attcttcagc aagtccagga tcgacgagtt caagcccagt gagaacatta tcgagaacta 3601 caagaagggg acccacaaga agggagcaaa tttcaacctg gccgactgcc acaaccttat 3661 cgacttcttt aagtcctcta tcagcaagca cgaagattgg tccaagttca acttccactt 3721 cagcgacacc tcgtcctacg aggacctcag cgacttctac cgcgaagtgg agcagcaggg 3781 ctactcaatt tccttctgcg acgtcagcgt ggagtacatc aacaagatgg ttgagaaggg 3841 tgatttgtat ctcttccaaa tctacaacaa ggacttctct gagttttcca agggaacccc 3901 aaatatgcat actctgtact ggaactccct tttcagcaag gagaacctca acaacattat 3961 ctacaagttg aacggccagg ctgagatctt cttcagaaag aagtcgctga attacaagcg 4021 tcctacccac ccggctcacc aggccatcaa gaacaagaac aagtgcaacg agaagaagga 4081 gtccattttc gactacgacc tcgtgaagga caagcgctac accgtcgata aattccagtt 4141 ccacgtgccc atcacgatga acttcaagag caccgggaac accaatatca accaacaagt 4201 catcgactac ctaaggactg aagatgacac ccacattatc ggcatcgacc gcggagagcg 4261 tcaccttctc tacctggttg tgattgactc tcacggcaag atcgtcgagc agttcacctt 4321 gaacgagatc gtgaacgagt acggtggcaa catctatcgc accaactacc acgacctcct 4381 ggacacaagg gagcagaatc gcgaaaaggc cagagagtcc tggcagacca ttgagaacat 4441 caaggagctt aaggagggat acatctcaca agtcatccac aagattaccg acctcatgca 4501 gaagtaccat gccgttgtgg tcttggaaga tctcaacatg ggctttatgc gtgggaggca 4561 gaaagtggag aagcaagtct accagaagtt cgaggagatg ctgatcaaca agctcaacta 4621 ccttgtgaac aagaaggcgg accagaatag cgccggaggc ttgctgcacg cttaccagct 4681 cacttccaag ttcgagagtt tccagaagct cggtaagcag agcggcttcc tgttctacat 4741 cccagcctgg aacacctcca agatcgaccc cgttacggga ttcgtcaacc ttttcgacac 4801 ccgctacgag tcgattgata aggccaaggc attcttcggc aagtttgact ctatccggta 4861 caacgccgac aaggactggt tcgagttcgc tttcgactac aacaacttca ccactaaggc 4921 cgaaggaacc cgtactaatt ggaccatctg cacctacggc tcccgcatta ggaccttcag 4981 aaaccaagcc aagaacagcc agtgggataa cgaggagatc gacttgacca aggcttacaa 5041 ggccttcttc gcgaagcacg ggatcaacat ctatgacaac attaaggagg ccatcgctat 5101 ggagacagaa aagtccttct ttgaggacct cctgcacctc cttaaattga ccctccagat 5161 gcgcaatagc atcaccggca cgaccaccga ctacctgatc tcacctgtgc acgattccaa 5221 gggtaacttc tacgacagcc gtatttgcga caactcgctc ccggccaacg ccgacgcaaa 5281 cggagcctac aacatcgcta ggaagggcct tatgttgatc cagcaaatca aggattccac 5341 ttctagcaat cgcttcaagt tctccccaat taccaacaag gactggctga tcttcgccca 5401 ggagaagccc tacctcaacg acaagcgccc tgctgccacc aagaaagctg gccaggccaa 5461 gaagaagaaa tgacccggga tcgttcaaac atttggcaat aaagtttctt aagattgaat 5521 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta 5581 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg 5641 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta 5701 tcgcgcgcgg tgtcatctat gttactagat cgctcgaatt cattgaatgt ccattgaagc 5761 agacgtgttg cgacaggtta gcgacgatgg gtgtagatgt gatgtgatgt gatggtgtgg 5821 ttcttccacg gcggcgtcct tgcggtggcg gagaagggga tatcccgaag gagcggcagc 5881 gggagagcac aagcagaaag ggtgcagtga gtgagtgggt ccagctgggt ggctggccga 5941 gtggacgcga ccgggtttcg agggggcggg ggagaaaagg gatggagcga gggatataac 6001 ccacatggaa tggaggtggg tgtgaaggcg ggtatatagg aaggtggagg acttacaacc 6061 gtaatttcta ctaagtgtag atgagacgtt cgaagcttct gtcgtctcag tcgacaggcc 6121 ttaagggcct gatctgtata aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 6661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 6721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 6781 acgctcagtg gaacgaaaac tcacgttaag ggattttggc catgagatca ccaatgctta 6841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 6901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 6961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 7021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 7081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 7141 gctgctggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 7201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 7261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 7321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 7381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 7441 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 7501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 7561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 7621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa ggg //