LOCUS pUbiZmU6pp-Cpf1 7658 bp DNA circular SYN 23-AUG-2018 DEFINITION pUbiZmU6pp-Cpf1 (D042): Ligation of Cpf1_BamXma* into C845pUbiZmU6pp-Cas-kozpp* ACCESSION pUbiZmU6pp-Cpf1 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT DNA Cloning Service, https://dna-cloning.com/vectors/crispr/pUbiZmU6pp-Cpf1 FEATURES Location/Qualifiers misc_feature 98..1606 /label="Ubi-int" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature 1085..1604 /label="Intron" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 1625..5407 /label="ORF-1" /note="color: #D9A400" /ApEinfo_fwdcolor="#D9A400" /ApEinfo_revcolor="#D9A400" misc_feature 1631..1651 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 5357..5404 /label="NLS" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 5413..5666 /label="NOS-T" /note="color: #B8322F" /ApEinfo_fwdcolor="#B8322F" /ApEinfo_revcolor="#B8322F" misc_feature 5676..5994 /label="U6 Pp" /note="color: #2F7A3E" /ApEinfo_fwdcolor="#2F7A3E" /ApEinfo_revcolor="#2F7A3E" misc_feature complement(5990..6000) /label="BsmBI site (CAAC)" /note="BsmBI recognition site CGTCTC and the 4-nt overhang it leaves" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 5996..6021 /label="targetRNA" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 6015..6025 /label="BsmBI site (GTTT)" /note="BsmBI recognition site CGTCTC and the 4-nt overhang it leaves" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" CDS 6022..6051 /label="hairpin" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature 6022..6104 /label="gRNA" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" misc_feature complement(6156..6820) /label="ColE1" /note="color: #8B8F86" /ApEinfo_fwdcolor="#8B8F86" /ApEinfo_revcolor="#8B8F86" misc_feature complement(join(6821..7658,1..25)) /label="Amp" /note="color: #7B5EA7" /ApEinfo_fwdcolor="#7B5EA7" /ApEinfo_revcolor="#7B5EA7" ORIGIN 1 cgacacggaa atgttgaata ctcatggctg acctcctatg cagatatctt atgagtgact 61 agaatagctc tcaagatcag gccttggcgg ccctagtctg cagtgcagcg tgacccggtc 121 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat 181 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt 241 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata 301 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac 361 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata 421 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata 481 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa 541 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga 601 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt 661 ttcgagtaga taatgccagc ctgttaaacg ccgtcgatcg acgagtctaa cggacaccaa 721 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 781 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 841 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 901 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 961 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 1021 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 1081 caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc 1141 ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt 1201 gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc 1261 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg 1321 cagacgggat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac 1381 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat 1441 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc 1501 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt 1561 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcaggtactg gatcctccat 1621 agcaatggct cccaagaaga agcgcaaggt gggcatccac ggagtcccag ccgcctccaa 1681 gctggagaag ttcaccaact gctacagcct ctctaaaacc ctccgtttca aggcaattcc 1741 tgttggtaag actcaggaga acatcgacaa caagaggctt ctcgtggagg acgaaaagag 1801 agccgaggat tacaagggcg tcaagaagct gctcgaccgc tactacttgt ccttcatcaa 1861 tgacgtgctt cactcgatca agctcaagaa cctgaacaac tacattagcc tcttccgtaa 1921 gaaaaccagg acagagaagg agaacaagga gttggaaaac cttgagatca atctccgcaa 1981 agagatcgcc aaggctttta aggggaacga gggctataag tccctgttca agaaggacat 2041 cattgagact atcctaccgg agttcctcga cgataaggac gaaatcgcct tggtcaactc 2101 attcaacgga ttcaccacgg cgttcaccgg cttcttcgac aaccgggaga atatgttcag 2161 cgaggaggcc aagtccacca gtatcgcttt tcgctgcatt aacgagaacc tgacccgtta 2221 catctccaac atggacatct tcgaaaaggt tgatgccatc ttcgacaagc acgaggtgca 2281 agagattaag gagaagatcc tcaacagcga ctacgacgtg gaggacttct tcgaaggaga 2341 gttcttcaac ttcgtcctta cccaggaggg catcgatgtc tacaacgcca ttatcggtgg 2401 cttcgttacc gagtctggag agaagatcaa ggggctcaat gagtacatca acctctacaa 2461 ccagaaaacc aagcagaagc tgcccaagtt taagccactt tacaagcaag tgctttccga 2521 cagggaaagc ctgtcgttct acggcgaggg ctacacatcc gacgaggagg tcttggaggt 2581 gttccgcaac accctcaaca agaacagcga aattttctcc tctatcaaga agctggagaa 2641 gctcttcaag aacttcgacg agtactcaag cgctggaatc ttcgtcaaga atggtcccgc 2701 catctccact atttccaagg acatcttcgg cgagtggaac gttatcagag ataagtggaa 2761 cgcagagtat gacgacatcc atcttaagaa gaaagccgtg gtcaccgaga agtacgagga 2821 cgatcgccgt aagagcttca agaagattgg atcgttttcc ttggaacagc tccaggagta 2881 cgccgacgct gacctgtctg tggtcgagaa gctcaaggag atcatcatac agaaggtaga 2941 cgaaatctac aaggtgtacg gcagctccga gaagttgttc gacgccgatt tcgtccttga 3001 gaagagcctc aagaagaacg acgcggttgt ggccattatg aaagacctgc tcgactccgt 3061 caagtcattc gagaactaca tcaaggcttt cttcggggag ggcaaggaga ctaacaggga 3121 cgaatcgttc tacggagatt tcgtgcttgc ctacgacatc ttgctcaagg ttgaccacat 3181 ctacgacgcc attcgcaatt acgtcaccca aaagccttat tccaaggata agttcaagct 3241 gtactttcag aacccgcagt tcatgggcgg ttgggacaag gacaaggaga ctgactaccg 3301 agctaccatc ctccgttacg gcagcaagta ctaccttgcc atcatggaca agaaatacgc 3361 aaagtgcctg caaaagatcg ataaggacga cgtgaacgga aactacgaga agattaacta 3421 caagttgctc ccaggcccca acaagatgct gcctaaggtg ttcttctcta agaagtggat 3481 ggcctactac aatccctccg aggacatcca gaagatttat aagaacggga ccttcaagaa 3541 aggagatatg ttcaacctca acgactgcca caagttgatc gacttcttca aggacagcat 3601 ctcccgctac ccaaagtgga gtaacgccta cgacttcaac tttagcgaga cagaaaagta 3661 caaggatatc gctggcttct acagggaggt cgaggagcag ggttacaagg tcagcttcga 3721 gtctgcctcg aagaaggagg ttgacaagct ggtggaagag ggcaagctct atatgttcca 3781 gatttacaac aaggacttct ccgacaagtc ccacggaacc ccgaatcttc acaccatgta 3841 cttcaagctc ttgttcgacg agaacaacca cggccaaatc agactttcag gcggggcgga 3901 gctcttcatg cgccgtgcct ccctgaagaa agaggaactc gtcgttcacc ccgctaacag 3961 cccaatcgcc aacaagaacc ctgataatcc caagaaaacc accacgttgt cctacgacgt 4021 gtataaggac aagaggttca gcgaggacca gtacgagctg cacatcccaa tcgccattaa 4081 caagtgcccc aagaacatct ttaagatcaa caccgaggtc cgcgtgcttc tcaagcatga 4141 cgataaccct tacgtcatcg gaattgaccg tggcgagagg aacctactct acatcgtggt 4201 cgtggacggt aagggaaata tcgttgagca gtactctttg aacgaaatca ttaacaactt 4261 caacggcatc cgcatcaaga cggactacca ctccctgctc gataagaaag agaaggagag 4321 attcgaggca cgccagaact ggacctcgat cgagaatatt aaggaactta aggctggcta 4381 catcagccaa gtcgtccaca agatttgcga gctggtggag aagtacgacg ccgtgatcgc 4441 cttggaggac ctcaactccg ggttcaagaa ctcacgggtt aaggtcgaga agcaggtgta 4501 ccagaagttc gagaagatgc tcatcgacaa gctgaactac atggtggaca agaaatccaa 4561 cccgtgcgcc accggaggcg ctcttaaggg ttaccaaatc accaacaagt tcgaatcttt 4621 caagagcatg tccactcaga acggcttcat cttctacatt cccgcctggc tcaccagcaa 4681 gatcgatcca tccaccggat ttgtcaattt gctcaagaca aagtacacct cgatcgcgga 4741 cagcaagaag ttcatctcca gtttcgaccg tattatgtac gttcccgagg aggacctgtt 4801 cgagttcgcc cttgactaca agaacttctc taggaccgat gctgactaca tcaagaagtg 4861 gaagttgtac tcctacggca accgcatccg catcttccgt aacccgaaga agaacaacgt 4921 gttcgactgg gaggaagtct gcctcacgag cgcctacaaa gagctgttca ataagtacgg 4981 aattaactat cagcagggcg acatcagggc cttgctttgc gagcaatccg ataaggcatt 5041 ttacagctca ttcatggccc tcatgtccct catgctccag atgcgcaaca gcatcaccgg 5101 gagaactgac gtggacttcc tgatctcgcc tgtcaagaac tccgacggca ttttctacga 5161 ctctcgtaac tacgaggctc aggagaacgc catccttcca aagaatgccg atgctaacgg 5221 tgcctacaac atcgcgagga aggttttgtg ggccatcgga cagttcaaga aggctgagga 5281 cgaaaagctg gacaaggtca agattgccat cagcaacaag gagtggttgg agtacgccca 5341 aacctccgtg aagcacaaac gccccgcagc caccaagaag gctggccagg ccaagaagaa 5401 gaagtaaccc gggatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt 5461 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt 5521 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta 5581 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc 5641 gcggtgtcat ctatgttact agatcgctcg aattcattga atgtccattg aagcagacgt 5701 gttgcgacag gttagcgacg atgggtgtag atgtgatgtg atgtgatggt gtggttcttc 5761 cacggcggcg tccttgcggt ggcggagaag gggatatccc gaaggagcgg cagcgggaga 5821 gcacaagcag aaagggtgca gtgagtgagt gggtccagct gggtggctgg ccgagtggac 5881 gcgaccgggt ttcgaggggg cgggggagaa aagggatgga gcgagggata taacccacat 5941 ggaatggagg tgggtgtgaa ggcgggtata taggaaggtg gaggacttac aaccgagacg 6001 ttcgaagctt ctgtcgtctc agttttagag ctagaaatag caagttaaaa taaggctagt 6061 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgtcgac aggccttaag 6121 ggcctgatct gtataaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 6601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6781 cagtggaacg aaaactcacg ttaagggatt ttggccatga gatcaccaat gcttaatcag 6841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 7141 tggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 7441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7621 aacaggaagg caaaatgccg caaaaaaggg aataaggg //