LOCUS       D029p9ioR-U10Cas       14739 bp    DNA     circular SYN 26-AUG-2020
DEFINITION  Ligation of B763p9ioR-35sD10Aoi* into C111p9ioR-Uq10CasWToi*
ACCESSION   D029p9ioR-U10Cas
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  C111p9ioR-Uq10CasWToi*
               Fragment ends: AfeI and BamHI
               Fragment size: 13216
            Insert molecule:  B763p9ioR-35sD10Aoi*
               Fragment ends: BamHI and AfeI
               Fragment size: 1523
            Sequencing primers	
            Ubq10-F	CTAGATCTGGTGTTAGTTTCTAGT
            CasF2	CCTTACTACGTCGGACCCTT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..1043
                     /gene="NptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1044..1230
                     /gene="int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1231..1734
                     /gene="NptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1748..2038
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2087..3562
                     /gene="Pro-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3191..3254
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3257..3560
                     /product="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3584..7786
                     /gene="D10A'"
                     /translation="MAPKKKRKVGIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVP
                     SKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIF
                     SNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVD
                     STDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
                     ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAE
                     DAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLS
                     ASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFI
                     KPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLK
                     DNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFI
                     ERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD
                     LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLD
                     NEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKL
                     INGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHI
                     ANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMK
                     RIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDH
                     IVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFD
                     NLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVI
                     TLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYK
                     VYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEI
                     VWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKK
                     YGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYK
                     EVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLK
                     GSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDL
                     SQLGGDKRPAATKKAGQAKKKK"
                     /product="Cas9 nickase"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Cas9D10A"
                     /SECDescr="Cas9 nickase"
     misc_feature    3590..3610
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3660
                     /product="D10A mut to C"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7736..7783
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7792..8045
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS-T"
                     /SECDescr="Nos-Terminator"
     misc_feature    8055..8499
                     /gene="U6At"
                     /product="Promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8500..8526
                     /product="targetRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             8527..8556
                     /product="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    8527..8609
                     /gene="gRNA"
                     /product="gRNA"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8695..8718
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9253..9950
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    10238..13025
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             13209..14219
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct
      781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt
      841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg
      901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg
      961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga
     1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat
     1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac
     1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg
     1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact
     1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct
     1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc
     1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc
     1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg
     1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt
     1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg
     1741 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     1801 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     1861 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     1921 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     1981 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga
     2041 agcctgcagg catgcaagct gatccactag aggccatggc ggccgctttc cttacattct
     2101 gagcctcttt ccttctaatc cactcatctg catcttcttg tgtccttact aatacctcat
     2161 tggttccaaa ttccctccct ttaagcacca gctcgtttct gttcttccac agcctcccaa
     2221 gtatccaagg gactaaagcc tccacattct tcagatcagg atattcttgt ttaagatgtt
     2281 gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc ccttcttcat
     2341 agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta ttaatgaaaa
     2401 aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc ccaaataaga
     2461 tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca cttgcctttt
     2521 ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa atcaataatc
     2581 atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc acaatatgtt
     2641 atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc acttgcatta
     2701 gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat tctagaaaat
     2761 acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt ttcagctcca
     2821 ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa cgatcgttaa
     2881 atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac gagtcagtaa
     2941 taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca
     3001 caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa
     3061 caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc
     3121 gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct
     3181 cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc tctcaattct
     3241 ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct tcgattttgt
     3301 tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa tcttagatcg
     3361 aagacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta gggtttcata
     3421 gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc ttcgatttgt
     3481 gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg tcgattaatc
     3541 tgagtttttc tgattaacag gtactagtgg atcctccata actatggccc ccaagaagaa
     3601 gcgcaaagtg ggaatccatg gcgtcccagc cgcagacaag aagtacagca ttggtctggc
     3661 catcgggacc aactccgtcg gctgggccgt tatcaccgat gagtacaaag tgccttctaa
     3721 gaagttcaaa gtcctcggaa acactgacag gcacagcatc aagaagaact tgattggcgc
     3781 ccttctcttc gactccggcg aaaccgctga ggccacccgt ctgaagcgca ccgccagaag
     3841 gcgctacacc cgtcgcaaga ataggatctg ctacctccag gagatcttca gcaacgagat
     3901 ggccaaagtg gacgattcct tctttcaccg tttggaggag agcttccttg tcgaggagga
     3961 caagaagcac gagcgccatc ccatcttcgg aaacattgtt gacgaggtgg cttaccacga
     4021 gaagtatcca actatctacc acctcagaaa gaagctggtg gactccaccg acaaggccga
     4081 tttgaggctc atctaccttg ccctcgctca catgatcaag ttccgcggtc acttcctgat
     4141 tgagggcgac ctcaaccccg acaactctga cgtcgataag ttgttcatcc aactggtcca
     4201 gacctacaac cagcttttcg aggagaatcc tatcaacgcc tccggggttg acgccaaggc
     4261 tattctcagc gcccgtctgt ccaagagccg caggcttgag aacttgatcg cccagctccc
     4321 aggagagaag aagaacggcc tcttcggaaa cctgatcgct ctctccttgg gccttacccc
     4381 taacttcaag tctaattttg acctcgccga ggacgccaag ctgcaactca gcaaggacac
     4441 ctacgatgac gacttggaca acctgctcgc ccagatcggc gatcagtacg ctgacctttt
     4501 cttggccgcc aagaacctct ccgacgctat tctgctctcc gacatccttc gcgtgaacac
     4561 tgagatcacc aaggccccac tgagcgcctc catgatcaag cgttacgacg aacaccacca
     4621 agatttgacc ctcctcaagg ctcttgtcag acagcagctg cccgagaagt acaaggagat
     4681 tttcttcgac cagagcaaga acggatacgc cggttacatc gacggcgggg cctcccaaga
     4741 ggagttctac aagttcatca agcccatctt ggagaagatg gacggcaccg aagagctgct
     4801 cgtgaagctc aaccgcgagg atcttttgag gaagcagcgt accttcgaca acggatctat
     4861 tccccaccag atccatctcg gcgagctgca cgctatcctc cgcaggcaag aggacttcta
     4921 tccattcctt aaggacaatc gcgagaagat tgaaaagatc ctcactttta gaatccctta
     4981 ctacgtcgga cccttggccc gtggcaacag ccgcttcgcc tggatgacca ggaagtccga
     5041 ggaaaccatc accccatgga acttcgagga ggtggttgac aagggggcca gcgctcagtc
     5101 cttcattgag cgcatgacca acttcgataa gaacctgccc aacgagaaag tcctcccaaa
     5161 gcactccctt ctgtacgagt acttcactgt ctacaatgag ttgaccaaag tgaagtacgt
     5221 gaccgagggt atgcgtaagc ctgccttcct cagcggcgag cagaagaagg ccatcgttga
     5281 cctcctgttc aagaccaaca ggaaagtcac cgtgaagcag cttaaggagg actacttcaa
     5341 gaagatcgag tgctttgact ctgtcgagat cagcggagtg gaggatcgct tcaacgcttc
     5401 cttgggcact taccacgacc tcctgaagat tatcaaggac aaggacttcc tcgacaacga
     5461 ggagaacgaa gatatcttgg aggacatcgt tcttaccctc accctgttcg aggacagaga
     5521 gatgattgag gaacgcctca agacctacgc ccacttgttc gacgataaag tcatgaagca
     5581 acttaagcgt aggcgctaca ccggatgggg caggctgtcc cgtaagctca tcaacggtat
     5641 ccgcgacaag cagagcggca agactattct cgacttcttg aagtccgacg gattcgccaa
     5701 tcgcaacttc atgcagctga tccacgacga ttctcttacc ttcaaggagg acatccaaaa
     5761 ggctcaagtg tccggccagg gcgacagcct ccacgagcac atcgccaacc tggccgggag
     5821 ccccgctatt aagaagggaa tcctccagac cgtcaaggtg gttgacgagt tggtcaaggt
     5881 gatgggcaga cataagccag agaacatcgt catcgagatg gccagggaaa accagaccac
     5941 ccagaaggga cagaagaact cccgtgagcg catgaagagg attgaggagg gcatcaagga
     6001 gcttggttcc cagatcctca aggagcaccc cgtggaaaac acccaactcc agaatgagaa
     6061 gctgtatttg tactaccttc agaacgggcg cgatatgtac gttgaccaag agctggacat
     6121 caaccgcctc agcgactacg acgtggatca cattgtccct cagtcctttc tcaaggacga
     6181 ctctatcgac aacaaggtgt tgactagaag cgataagaac cgcggcaagt ccgacaacgt
     6241 cccatccgag gaggtggtca agaagatgaa gaattactgg aggcagctgc ttaacgccaa
     6301 gctcatcacc cagcgcaagt tcgacaactt gaccaaggcc gagcgtggag gcctcagcga
     6361 actggacaag gctggattca tcaagaggca acttgttgaa acccgccaga ttaccaagca
     6421 cgtggcccag atcctcgact cccgtatgaa cactaagtac gatgagaacg acaagctgat
     6481 ccgcgaggtc aaagtgatta ccctcaagag caagctcgtg tctgacttca gaaaggactt
     6541 ccaattctac aaggttaggg agatcaacaa ctaccaccac gcccacgatg cttaccttaa
     6601 tgccgtggtc ggcaccgcct tgatcaagaa gtaccccaag ctggagtccg agttcgtgta
     6661 tggtgactac aaggtctacg acgttcgcaa gatgatcgct aagagcgagc aggagattgg
     6721 caaggccacc gccaagtact tcttctactc caacatcatg aacttcttta agaccgagat
     6781 cactttggct aacggggaga tccgtaagcg ccctctcatt gaaaccaacg gagaaaccgg
     6841 cgagatcgtg tgggacaagg gcagggactt cgccaccgtc agaaaagtgc tgagcatgcc
     6901 ccaagtcaac atcgtgaaga aaaccgaggt tcagactgga ggcttctcca aggagtctat
     6961 cctcccaaag cgcaattccg ataagttgat tgcccgtaag aaggactggg accccaagaa
     7021 gtacggtgga ttcgacagcc caaccgtcgc ctactccgtg cttgtcgtgg ctaaagttga
     7081 gaagggcaag agcaagaagc tcaagtccgt caaggagctg ctcgggatca ccatcatgga
     7141 gcgcagctcc ttcgagaaga accctattga tttccttgag gccaagggct acaaggaagt
     7201 gaagaaggac ttgatcatca agctccccaa gtactctctg ttcgagcttg agaacggaag
     7261 gaagcgtatg ctcgcctccg ctggcgagct gcaaaaggga aacgagttgg ccctcccaag
     7321 caagtacgtc aacttcctgt acctcgcctc ccactatgag aagctcaagg gcagccccga
     7381 ggacaacgaa cagaagcagt tgtttgtgga gcagcataag cactaccttg acgagatcat
     7441 tgagcagatc agcgagttct ccaagcgcgt tatcctggct gacgccaatc tcgataaagt
     7501 cctttctgcc tacaacaagc acagggacaa gcctatcaga gagcaggctg aaaacattat
     7561 ccacttgttc accctcacca acctgggtgc cccagccgcc ttcaagtact tcgacactac
     7621 catcgaccgc aagcgttaca cctccaccaa ggaagtgctc gatgctaccc ttatccacca
     7681 gagcattact gggttgtacg aaaccaggat cgacctgtcc caactcggcg gagacaagcg
     7741 ccccgccgcc accaagaagg ctggccaggc caagaagaag aagtaacccg ggatcgttca
     7801 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     7861 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     7921 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     7981 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     8041 gatcgctcga attccttcgt tgaacaacgg aaactcgact tgccttccgc acaatacatc
     8101 atttcttctt agcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct
     8161 tacattttct tgaaccgtag ctttcgtttt cttcttttta actttccatt cggagttttt
     8221 gtatcttgtt tcatagtttg tcccaggatt agaatgatta ggcatcgaac cttcaagaat
     8281 ttgattgaat aaaacatctt cattcttaag atatgaagat aatcttcaaa aggcccctgg
     8341 gaatctgaaa gaagagaagc aggcccattt atatgggaaa gaacaatagt atttcttata
     8401 taggcccatt taagttgaaa acaatcttca aaagtcccac atcgcttaga taagaaaacg
     8461 aagctgagtt tatatacagc tagagtcgaa gtagtgattg agacgttcga agcttctgtc
     8521 gtctcagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     8581 aaaagtggca ccgagtcggt gctttttttg tcgacaggcc ttaagggcca gatcttgggc
     8641 ccggtacccg atcagattgt cgtttcccgc cttcggttta aactatcagt gtttgacagg
     8701 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     8761 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     8821 cctcgggagt gcttggcatt ccgtgcgata atgacttctg ttcaaccacc caaacgtcgg
     8881 aaagcctgac gacggagcag cattccaaaa agatcccttg gctcgtctgg gtcggctaga
     8941 aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt cgcggacgta gcgcagcgcc
     9001 gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg tgatctgctt gtcgctcttt
     9061 cggccgacgt cctggccagt catcacgcgc caaagttccg tcacaggatg atctggcgcg
     9121 agttgctgga tctcgccttc aatccgggtc tgtggcggga actccacgaa aatatccgaa
     9181 cgcagcaaga tatcgcggtg catctcggtc ttgcctgggc agtcgccgcc gacgccgttg
     9241 atgtggacgc cgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9301 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9361 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9421 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9481 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9541 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9601 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9661 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9721 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10021 gcgttatccc ctgattctgt ggataaccga ttaccgcctt tgagtgagct gataccgctc
    10081 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
    10141 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    10201 gtacaatctg ctctgatgcc gcatagttaa gccagtaacc gtgcggctgc atgaaatcct
    10261 ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac
    10321 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
    10381 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
    10441 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
    10501 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
    10561 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
    10621 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
    10681 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
    10741 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
    10801 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
    10861 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
    10921 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
    10981 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    11041 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    11101 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    11161 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    11221 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    11281 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    11341 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
    11401 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
    11461 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
    11521 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc
    11581 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
    11641 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
    11701 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
    11761 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
    11821 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
    11881 gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
    11941 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
    12001 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
    12061 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
    12121 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
    12181 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
    12241 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
    12301 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
    12361 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
    12421 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
    12481 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
    12541 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
    12601 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
    12661 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
    12721 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
    12781 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
    12841 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
    12901 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
    12961 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
    13021 gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
    13081 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
    13141 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
    13201 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
    13261 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
    13321 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
    13381 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
    13441 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
    13501 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
    13561 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
    13621 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
    13681 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
    13741 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
    13801 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
    13861 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
    13921 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
    13981 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
    14041 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
    14101 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
    14161 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
    14221 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
    14281 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
    14341 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt
    14401 cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg
    14461 aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg
    14521 gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg
    14581 tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt
    14641 gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa
    14701 ccaacatgca agctccaccg ggtgcaaagc ggcagcggc
//
