LOCUS       D023p7D35s-C9wt-       12916 bp    DNA     circular SYN 26-AUG-2020
DEFINITION  Ligation of C035p7ioR-Uq10CasWToi* into D011p7D35s*
ACCESSION   D023p7D35s-C9wt-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  D011p7D35s*
               Fragment ends: SfiI
               Fragment size: 6377
            Insert molecule:  C035p7ioR-Uq10CasWToi*
               Fragment ends: SfiI
               Fragment size: 6539
            Sequencing primers:
            BarF	CTTCTGGCAGCTCGACTTCT
            SphR	GGGTGGTTGAACAGAAGTCAT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB'"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LB"
                     /SECDescr="Left Border"
     misc_feature    169..584
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             605..838
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    839..1027
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="STLS1"
                     /SECDescr="Intron"
     CDS             1028..1343
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1356..1646
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1672..3147
                     /gene="Pro-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2776..2839
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2842..3145
                     /product="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3169..7365
                     /gene="'Cas9WT"
                     /translation="MAPKKKRKVGIHGAPDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST
                     DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS
                     MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP
                     ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN
                     REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER
                     MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
                     FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE
                     ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN
                     GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN
                     LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI
                     EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV
                     PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
                     TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL
                     KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
                     DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW
                     DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG
                     GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV
                     KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS
                     PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA
                     ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ
                     LGGDKRPAATKKAGQAKKKK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Cas9WToi"
                     /SECDescr="no intronsequence"
     misc_feature    3175..3195
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7315..7362
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7371..7624
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TNOS"
                     /SECDescr="Nos-Terminator"
     misc_feature    7634..8078
                     /gene="U6At"
                     /product="U6At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8079..8105
                     /product="targetRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             8106..8135
                     /product="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    8106..8188
                     /gene="gRNA"
                     /product="gRNA - scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8234..8836
                     /product="Right Border Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8279..8302
                     /gene="RB"
                     /product="RB"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="RB"
                     /SECDescr="RB"
     misc_feature    8850..9508
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9537..11500
                     /gene="''pVS1 ORI'''"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pVS1"
                     /SECDescr="pVS1 ORI"
     CDS             9863..11038
                     /gene="ParB"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="ParB"
     CDS             11689..12699
                     /gene="strep/spec"
                     /product="Strep/Spec resistence"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Sm/Sp"
                     /SECDescr="Strep/Spec resistence"
     misc_feature    join(12767..12916,1..163)
                     /product="Left Border Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc
      181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
      241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
      361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
      421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat
      601 aactatgggc cccgagcgca ggcccgccga catccgccgt gccaccgagg ccgacatgcc
      661 agccgtctgc accatcgtga accactacat cgagacttcc acggtcaact tccgcacaga
      721 gccgcaggag cctcaggagt ggaccgacga cctcgtgagg ctgcgcgagc gctacccctg
      781 gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggt
      841 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
      901 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
      961 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     1021 tgtgcaggca cgcaacgcct acgactggac ggcggagagc accgtctacg tgtccccacg
     1081 ccaccagcgc accggcctgg gctccacgct ctacacccac ctgctcaaga gcctggaggc
     1141 ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc aacgacccga gcgtccgtat
     1201 gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg gccgcgggct tcaagcacgg
     1261 caactggcac gacgtgggct tctggcagct cgacttctcc ctgcctgtcc caccccgccc
     1321 ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt atggcattgg gaaaactgtt
     1381 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc
     1441 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat
     1501 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata
     1561 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc
     1621 gaagttaata tgaggagtaa aacactgaag cctgcaggcc ttggcggccg ctttccttac
     1681 attctgagcc tctttccttc taatccactc atctgcatct tcttgtgtcc ttactaatac
     1741 ctcattggtt ccaaattccc tccctttaag caccagctcg tttctgttct tccacagcct
     1801 cccaagtatc caagggacta aagcctccac attcttcaga tcaggatatt cttgtttaag
     1861 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc
     1921 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat
     1981 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa
     2041 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc
     2101 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa
     2161 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat
     2221 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg
     2281 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag
     2341 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag
     2401 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc
     2461 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc
     2521 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca
     2581 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt
     2641 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct
     2701 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa
     2761 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca
     2821 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat
     2881 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta
     2941 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt
     3001 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga
     3061 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat
     3121 taatctgagt ttttctgatt aacaggtact agtggatcct ccataactat ggcccccaag
     3181 aagaagcgca aagtgggaat ccacggagct ccagacaaga agtacagcat tggtctggac
     3241 atcgggacca actccgtcgg ctgggccgtt atcaccgatg agtacaaagt gccttctaag
     3301 aagttcaaag tcctcggaaa cactgacagg cacagcatca agaagaactt gattggcgcc
     3361 cttctcttcg actccggcga aaccgctgag gccacccgtc tgaagcgcac cgccagaagg
     3421 cgctacaccc gtcgcaagaa taggatctgc tacctccagg agatcttcag caacgagatg
     3481 gccaaagtgg acgattcctt ctttcaccgt ttggaggaga gcttccttgt cgaggaggac
     3541 aagaagcacg agcgccatcc catcttcgga aacattgttg acgaggtggc ttaccacgag
     3601 aagtatccaa ctatctacca cctcagaaag aagctggtgg actccaccga caaggccgat
     3661 ttgaggctca tctaccttgc cctcgctcac atgatcaagt tccgcggtca cttcctgatt
     3721 gagggcgacc tcaaccccga caactctgac gtcgataagt tgttcatcca actggtccag
     3781 acctacaacc agcttttcga ggagaatcct atcaacgcct ccggggttga cgccaaggct
     3841 attctcagcg cccgtctgtc caagagccgc aggcttgaga acttgatcgc ccagctccca
     3901 ggagagaaga agaacggcct cttcggaaac ctgatcgctc tctccttggg ccttacccct
     3961 aacttcaagt ctaattttga cctcgccgag gacgccaagc tgcaactcag caaggacacc
     4021 tacgatgacg acttggacaa cctgctcgcc cagatcggcg atcagtacgc tgaccttttc
     4081 ttggccgcca agaacctctc cgacgctatt ctgctctccg acatccttcg cgtgaacact
     4141 gagatcacca aggccccact gagcgcctcc atgatcaagc gttacgacga acaccaccaa
     4201 gatttgaccc tcctcaaggc tcttgtcaga cagcagctgc ccgagaagta caaggagatt
     4261 ttcttcgacc agagcaagaa cggatacgcc ggttacatcg acggcggggc ctcccaagag
     4321 gagttctaca agttcatcaa gcccatcttg gagaagatgg acggcaccga agagctgctc
     4381 gtgaagctca accgcgagga tcttttgagg aagcagcgta ccttcgacaa cggatctatt
     4441 ccccaccaga tccatctcgg cgagctgcac gctatcctcc gcaggcaaga ggacttctat
     4501 ccattcctta aggacaatcg cgagaagatt gaaaagatcc tcacttttag aatcccttac
     4561 tacgtcggac ccttggcccg tggcaacagc cgcttcgcct ggatgaccag gaagtccgag
     4621 gaaaccatca ccccatggaa cttcgaggag gtggttgaca agggggccag cgctcagtcc
     4681 ttcattgagc gcatgaccaa cttcgataag aacctgccca acgagaaagt cctcccaaag
     4741 cactcccttc tgtacgagta cttcactgtc tacaatgagt tgaccaaagt gaagtacgtg
     4801 accgagggta tgcgtaagcc tgccttcctc agcggcgagc agaagaaggc catcgttgac
     4861 ctcctgttca agaccaacag gaaagtcacc gtgaagcagc ttaaggagga ctacttcaag
     4921 aagatcgagt gctttgactc tgtcgagatc agcggagtgg aggatcgctt caacgcttcc
     4981 ttgggcactt accacgacct cctgaagatt atcaaggaca aggacttcct cgacaacgag
     5041 gagaacgaag atatcttgga ggacatcgtt cttaccctca ccctgttcga ggacagagag
     5101 atgattgagg aacgcctcaa gacctacgcc cacttgttcg acgataaagt catgaagcaa
     5161 cttaagcgta ggcgctacac cggatggggc aggctgtccc gtaagctcat caacggtatc
     5221 cgcgacaagc agagcggcaa gactattctc gacttcttga agtccgacgg attcgccaat
     5281 cgcaacttca tgcagctgat ccacgacgat tctcttacct tcaaggagga catccaaaag
     5341 gctcaagtgt ccggccaggg cgacagcctc cacgagcaca tcgccaacct ggccgggagc
     5401 cccgctatta agaagggaat cctccagacc gtcaaggtgg ttgacgagtt ggtcaaggtg
     5461 atgggcagac ataagccaga gaacatcgtc atcgagatgg ccagggaaaa ccagaccacc
     5521 cagaagggac agaagaactc ccgtgagcgc atgaagagga ttgaggaggg catcaaggag
     5581 cttggttccc agatcctcaa ggagcacccc gtggaaaaca cccaactcca gaatgagaag
     5641 ctgtatttgt actaccttca gaacgggcgc gatatgtacg ttgaccaaga gctggacatc
     5701 aaccgcctca gcgactacga cgtggatcac attgtccctc agtcctttct caaggacgac
     5761 tctatcgaca acaaggtgtt gactagaagc gataagaacc gcggcaagtc cgacaacgtc
     5821 ccatccgagg aggtggtcaa gaagatgaag aattactgga ggcagctgct taacgccaag
     5881 ctcatcaccc agcgcaagtt cgacaacttg accaaggccg agcgtggagg cctcagcgaa
     5941 ctggacaagg ctggattcat caagaggcaa cttgttgaaa cccgccagat taccaagcac
     6001 gtggcccaga tcctcgactc ccgtatgaac actaagtacg atgagaacga caagctgatc
     6061 cgcgaggtca aagtgattac cctcaagagc aagctcgtgt ctgacttcag aaaggacttc
     6121 caattctaca aggttaggga gatcaacaac taccaccacg cccacgatgc ttaccttaat
     6181 gccgtggtcg gcaccgcctt gatcaagaag taccccaagc tggagtccga gttcgtgtat
     6241 ggtgactaca aggtctacga cgttcgcaag atgatcgcta agagcgagca ggagattggc
     6301 aaggccaccg ccaagtactt cttctactcc aacatcatga acttctttaa gaccgagatc
     6361 actttggcta acggggagat ccgtaagcgc cctctcattg aaaccaacgg agaaaccggc
     6421 gagatcgtgt gggacaaggg cagggacttc gccaccgtca gaaaagtgct gagcatgccc
     6481 caagtcaaca tcgtgaagaa aaccgaggtt cagactggag gcttctccaa ggagtctatc
     6541 ctcccaaagc gcaattccga taagttgatt gcccgtaaga aggactggga ccccaagaag
     6601 tacggtggat tcgacagccc aaccgtcgcc tactccgtgc ttgtcgtggc taaagttgag
     6661 aagggcaaga gcaagaagct caagtccgtc aaggagctgc tcgggatcac catcatggag
     6721 cgcagctcct tcgagaagaa ccctattgat ttccttgagg ccaagggcta caaggaagtg
     6781 aagaaggact tgatcatcaa gctccccaag tactctctgt tcgagcttga gaacggaagg
     6841 aagcgtatgc tcgcctccgc tggcgagctg caaaagggaa acgagttggc cctcccaagc
     6901 aagtacgtca acttcctgta cctcgcctcc cactatgaga agctcaaggg cagccccgag
     6961 gacaacgaac agaagcagtt gtttgtggag cagcataagc actaccttga cgagatcatt
     7021 gagcagatca gcgagttctc caagcgcgtt atcctggctg acgccaatct cgataaagtc
     7081 ctttctgcct acaacaagca cagggacaag cctatcagag agcaggctga aaacattatc
     7141 cacttgttca ccctcaccaa cctgggtgcc ccagccgcct tcaagtactt cgacactacc
     7201 atcgaccgca agcgttacac ctccaccaag gaagtgctcg atgctaccct tatccaccag
     7261 agcattactg ggttgtacga aaccaggatc gacctgtccc aactcggcgg agacaagcgc
     7321 cccgccgcca ccaagaaggc tggccaggcc aagaagaaga agtaacccgg gatcgttcaa
     7381 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     7441 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     7501 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     7561 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     7621 atcgctcgaa ttccttcgtt gaacaacgga aactcgactt gccttccgca caatacatca
     7681 tttcttctta gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt
     7741 acattttctt gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg
     7801 tatcttgttt catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt
     7861 tgattgaata aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg
     7921 aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat
     7981 aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga
     8041 agctgagttt atatacagct agagtcgaag tagtgattga gacgttcgaa gcttctgtcg
     8101 tctcagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     8161 aaagtggcac cgagtcggtg ctttttttgt cgacaggcct taacggccag atctgctggg
     8221 ccctgtggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga
     8281 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     8341 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     8401 ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg
     8461 tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc
     8521 tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag
     8581 cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct
     8641 ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg
     8701 cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc
     8761 cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc
     8821 gttgatgtgg acgccggctt agctcatggc caaaatccct taacgtgagt tttcgttcca
     8881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8941 cgtaatctgc tgcttgcaaa caaaaaaacc atcgctacca gcggtggttt gtttgccgga
     9001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9061 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9241 ggggggttcg tgctcacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttc ctaagggtta
     9541 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggtc gtccgagcgc
     9601 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     9661 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     9721 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     9781 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     9841 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     9901 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     9961 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
    10021 cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg aggcagaagc
    10081 acgccccggt gaatcgtggc aagccgccgc tgatcgaatc cgcaaagaat cccggcaacc
    10141 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
    10201 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
    10261 agtattccgc ctctcgaaac gtgatcgccg ggcgggcgaa gtcatccgct acgaactgcc
    10321 ggacggtcat gtcgaggtga gcgcaggccc ggcgggcatg gcctccgtct gggattatga
    10381 cctcgttctg atggccgtgt cgcaccttac ggaaagcatg aatcgctacc gggagggcaa
    10441 gggtgataag cccggtcgcg tattccgtcc gcatgtggcc gacgttctga aattctgtcg
    10501 ccgggcggat ggcggcaagc aaaaggacga tctcgtcgaa acctgcatcc gcctgaacac
    10561 gacccacgtc gccatgcagc gcaccaagaa agcgaagaat ggccgtcttg tgactgtctc
    10621 cgagggcgaa gccctgatct cgcgctacaa gatcgtaaag agcgaaaccg ggcggcccga
    10681 gtacatcgag atcgagcttg ctgattggat gtaccgcgag atcacagaag gcaagaaccc
    10741 ggacgtgctg acggttcacc ccgattactt tttgattgat cccggcatcg gtcgttttct
    10801 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
    10861 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
    10921 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
    10981 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
    11041 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aagctctctt
    11101 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
    11161 cattgggaac ccaaagccgt acattgggaa ccgggcacgc atgtaagtga ctgatataaa
    11221 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
    11281 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaatagctgc aaaaagcgcc
    11341 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgtggccgc
    11401 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
    11461 gccgtcgcca ctcgaccgcc gccgcccaca tcaaggcacc tcagcggtgg gtatgcctga
    11521 cgatgcgtgc agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
    11581 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
    11641 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
    11701 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
    11761 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
    11821 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
    11881 agtcgcccta aaacaaagtt aaacatcatg cgtgaagcgg tgatcgccga agtatcgact
    11941 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
    12001 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
    12061 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
    12121 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
    12181 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
    12241 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
    12301 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
    12361 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
    12421 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
    12481 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
    12541 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
    12601 cttggacaag aagaagatcg cttggcctct cgcgcagatc agttggaaga atttgtccac
    12661 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
    12721 cgacgccgct tcgcggcgcg gcttaactca agcgttagac ggaccgccca tgtggatcac
    12781 tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg
    12841 aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt
    12901 gcaaagcggc agcggc
//
