LOCUS       D784p7D35s-C9wt-       12771 bp    DNA     circular SYN 26-AUG-2020
DEFINITION  Ligation of U6Gm-Xma-Kpn* into D024p7D35s-C9wt-U6Gm*
ACCESSION   D784p7D35s-C9wt-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  D024p7D35s-C9wt-U6Gm*
               Fragment ends: KpnI and XmaI
               Fragment size: 12051
            Insert molecule:  U6Gm-Xma-Kpn*
               Fragment ends: XmaI and KpnI
               Fragment size: 720
            Sequencing primers:
            SphR	GGGTGGTTGAACAGAAGTCAT
            Caseq5     CGAGCTTGAGAACGGAAGG
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB'"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LB"
                     /SECDescr="Left Border"
     misc_feature    169..584
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             605..838
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    839..1027
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="STLS1"
                     /SECDescr="Intron"
     CDS             1028..1343
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1356..1646
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1672..3147
                     /gene="Pro-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2776..2839
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2842..3145
                     /product="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3169..7365
                     /gene="'Cas9WT"
                     /translation="MAPKKKRKVGIHGAPDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST
                     DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS
                     MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP
                     ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN
                     REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER
                     MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
                     FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE
                     ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN
                     GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN
                     LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI
                     EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV
                     PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
                     TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL
                     KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
                     DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW
                     DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG
                     GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV
                     KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS
                     PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA
                     ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ
                     LGGDKRPAATKKAGQAKKKK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Cas9WToi"
                     /SECDescr="no intronsequence"
     misc_feature    3175..3195
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7315..7362
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7371..7624
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TNOS"
                     /SECDescr="Nos-Terminator"
     misc_feature    7634..7938
                     /gene="U6Gm"
                     /product="U6Gm"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             7939..7965
                     /product="targetRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             7966..7995
                     /product="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    7966..8048
                     /gene="gRNA"
                     /product="gRNA - scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8089..8691
                     /product="Right Border Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8134..8157
                     /gene="RB"
                     /product="RB"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="RB"
                     /SECDescr="RB"
     misc_feature    8705..9363
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9392..11355
                     /gene="''pVS1 ORI'''"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pVS1"
                     /SECDescr="pVS1 ORI"
     CDS             9718..10893
                     /gene="ParB"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="ParB"
     CDS             11544..12554
                     /gene="strep/spec"
                     /product="Strep/Spec resistence"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Sm/Sp"
                     /SECDescr="Strep/Spec resistence"
     misc_feature    join(12622..12771,1..163)
                     /product="Left Border Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc
      181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
      241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
      361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
      421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat
      601 aactatgggc cccgagcgca ggcccgccga catccgccgt gccaccgagg ccgacatgcc
      661 agccgtctgc accatcgtga accactacat cgagacttcc acggtcaact tccgcacaga
      721 gccgcaggag cctcaggagt ggaccgacga cctcgtgagg ctgcgcgagc gctacccctg
      781 gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggt
      841 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
      901 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
      961 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     1021 tgtgcaggca cgcaacgcct acgactggac ggcggagagc accgtctacg tgtccccacg
     1081 ccaccagcgc accggcctgg gctccacgct ctacacccac ctgctcaaga gcctggaggc
     1141 ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc aacgacccga gcgtccgtat
     1201 gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg gccgcgggct tcaagcacgg
     1261 caactggcac gacgtgggct tctggcagct cgacttctcc ctgcctgtcc caccccgccc
     1321 ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt atggcattgg gaaaactgtt
     1381 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc
     1441 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat
     1501 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata
     1561 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc
     1621 gaagttaata tgaggagtaa aacactgaag cctgcaggcc ttggcggccg ctttccttac
     1681 attctgagcc tctttccttc taatccactc atctgcatct tcttgtgtcc ttactaatac
     1741 ctcattggtt ccaaattccc tccctttaag caccagctcg tttctgttct tccacagcct
     1801 cccaagtatc caagggacta aagcctccac attcttcaga tcaggatatt cttgtttaag
     1861 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc
     1921 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat
     1981 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa
     2041 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc
     2101 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa
     2161 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat
     2221 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg
     2281 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag
     2341 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag
     2401 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc
     2461 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc
     2521 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca
     2581 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt
     2641 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct
     2701 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa
     2761 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca
     2821 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat
     2881 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta
     2941 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt
     3001 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga
     3061 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat
     3121 taatctgagt ttttctgatt aacaggtact agtggatcct ccataactat ggcccccaag
     3181 aagaagcgca aagtgggaat ccacggagct ccagacaaga agtacagcat tggtctggac
     3241 atcgggacca actccgtcgg ctgggccgtt atcaccgatg agtacaaagt gccttctaag
     3301 aagttcaaag tcctcggaaa cactgacagg cacagcatca agaagaactt gattggcgcc
     3361 cttctcttcg actccggcga aaccgctgag gccacccgtc tgaagcgcac cgccagaagg
     3421 cgctacaccc gtcgcaagaa taggatctgc tacctccagg agatcttcag caacgagatg
     3481 gccaaagtgg acgattcctt ctttcaccgt ttggaggaga gcttccttgt cgaggaggac
     3541 aagaagcacg agcgccatcc catcttcgga aacattgttg acgaggtggc ttaccacgag
     3601 aagtatccaa ctatctacca cctcagaaag aagctggtgg actccaccga caaggccgat
     3661 ttgaggctca tctaccttgc cctcgctcac atgatcaagt tccgcggtca cttcctgatt
     3721 gagggcgacc tcaaccccga caactctgac gtcgataagt tgttcatcca actggtccag
     3781 acctacaacc agcttttcga ggagaatcct atcaacgcct ccggggttga cgccaaggct
     3841 attctcagcg cccgtctgtc caagagccgc aggcttgaga acttgatcgc ccagctccca
     3901 ggagagaaga agaacggcct cttcggaaac ctgatcgctc tctccttggg ccttacccct
     3961 aacttcaagt ctaattttga cctcgccgag gacgccaagc tgcaactcag caaggacacc
     4021 tacgatgacg acttggacaa cctgctcgcc cagatcggcg atcagtacgc tgaccttttc
     4081 ttggccgcca agaacctctc cgacgctatt ctgctctccg acatccttcg cgtgaacact
     4141 gagatcacca aggccccact gagcgcctcc atgatcaagc gttacgacga acaccaccaa
     4201 gatttgaccc tcctcaaggc tcttgtcaga cagcagctgc ccgagaagta caaggagatt
     4261 ttcttcgacc agagcaagaa cggatacgcc ggttacatcg acggcggggc ctcccaagag
     4321 gagttctaca agttcatcaa gcccatcttg gagaagatgg acggcaccga agagctgctc
     4381 gtgaagctca accgcgagga tcttttgagg aagcagcgta ccttcgacaa cggatctatt
     4441 ccccaccaga tccatctcgg cgagctgcac gctatcctcc gcaggcaaga ggacttctat
     4501 ccattcctta aggacaatcg cgagaagatt gaaaagatcc tcacttttag aatcccttac
     4561 tacgtcggac ccttggcccg tggcaacagc cgcttcgcct ggatgaccag gaagtccgag
     4621 gaaaccatca ccccatggaa cttcgaggag gtggttgaca agggggccag cgctcagtcc
     4681 ttcattgagc gcatgaccaa cttcgataag aacctgccca acgagaaagt cctcccaaag
     4741 cactcccttc tgtacgagta cttcactgtc tacaatgagt tgaccaaagt gaagtacgtg
     4801 accgagggta tgcgtaagcc tgccttcctc agcggcgagc agaagaaggc catcgttgac
     4861 ctcctgttca agaccaacag gaaagtcacc gtgaagcagc ttaaggagga ctacttcaag
     4921 aagatcgagt gctttgactc tgtcgagatc agcggagtgg aggatcgctt caacgcttcc
     4981 ttgggcactt accacgacct cctgaagatt atcaaggaca aggacttcct cgacaacgag
     5041 gagaacgaag atatcttgga ggacatcgtt cttaccctca ccctgttcga ggacagagag
     5101 atgattgagg aacgcctcaa gacctacgcc cacttgttcg acgataaagt catgaagcaa
     5161 cttaagcgta ggcgctacac cggatggggc aggctgtccc gtaagctcat caacggtatc
     5221 cgcgacaagc agagcggcaa gactattctc gacttcttga agtccgacgg attcgccaat
     5281 cgcaacttca tgcagctgat ccacgacgat tctcttacct tcaaggagga catccaaaag
     5341 gctcaagtgt ccggccaggg cgacagcctc cacgagcaca tcgccaacct ggccgggagc
     5401 cccgctatta agaagggaat cctccagacc gtcaaggtgg ttgacgagtt ggtcaaggtg
     5461 atgggcagac ataagccaga gaacatcgtc atcgagatgg ccagggaaaa ccagaccacc
     5521 cagaagggac agaagaactc ccgtgagcgc atgaagagga ttgaggaggg catcaaggag
     5581 cttggttccc agatcctcaa ggagcacccc gtggaaaaca cccaactcca gaatgagaag
     5641 ctgtatttgt actaccttca gaacgggcgc gatatgtacg ttgaccaaga gctggacatc
     5701 aaccgcctca gcgactacga cgtggatcac attgtccctc agtcctttct caaggacgac
     5761 tctatcgaca acaaggtgtt gactagaagc gataagaacc gcggcaagtc cgacaacgtc
     5821 ccatccgagg aggtggtcaa gaagatgaag aattactgga ggcagctgct taacgccaag
     5881 ctcatcaccc agcgcaagtt cgacaacttg accaaggccg agcgtggagg cctcagcgaa
     5941 ctggacaagg ctggattcat caagaggcaa cttgttgaaa cccgccagat taccaagcac
     6001 gtggcccaga tcctcgactc ccgtatgaac actaagtacg atgagaacga caagctgatc
     6061 cgcgaggtca aagtgattac cctcaagagc aagctcgtgt ctgacttcag aaaggacttc
     6121 caattctaca aggttaggga gatcaacaac taccaccacg cccacgatgc ttaccttaat
     6181 gccgtggtcg gcaccgcctt gatcaagaag taccccaagc tggagtccga gttcgtgtat
     6241 ggtgactaca aggtctacga cgttcgcaag atgatcgcta agagcgagca ggagattggc
     6301 aaggccaccg ccaagtactt cttctactcc aacatcatga acttctttaa gaccgagatc
     6361 actttggcta acggggagat ccgtaagcgc cctctcattg aaaccaacgg agaaaccggc
     6421 gagatcgtgt gggacaaggg cagggacttc gccaccgtca gaaaagtgct gagcatgccc
     6481 caagtcaaca tcgtgaagaa aaccgaggtt cagactggag gcttctccaa ggagtctatc
     6541 ctcccaaagc gcaattccga taagttgatt gcccgtaaga aggactggga ccccaagaag
     6601 tacggtggat tcgacagccc aaccgtcgcc tactccgtgc ttgtcgtggc taaagttgag
     6661 aagggcaaga gcaagaagct caagtccgtc aaggagctgc tcgggatcac catcatggag
     6721 cgcagctcct tcgagaagaa ccctattgat ttccttgagg ccaagggcta caaggaagtg
     6781 aagaaggact tgatcatcaa gctccccaag tactctctgt tcgagcttga gaacggaagg
     6841 aagcgtatgc tcgcctccgc tggcgagctg caaaagggaa acgagttggc cctcccaagc
     6901 aagtacgtca acttcctgta cctcgcctcc cactatgaga agctcaaggg cagccccgag
     6961 gacaacgaac agaagcagtt gtttgtggag cagcataagc actaccttga cgagatcatt
     7021 gagcagatca gcgagttctc caagcgcgtt atcctggctg acgccaatct cgataaagtc
     7081 ctttctgcct acaacaagca cagggacaag cctatcagag agcaggctga aaacattatc
     7141 cacttgttca ccctcaccaa cctgggtgcc ccagccgcct tcaagtactt cgacactacc
     7201 atcgaccgca agcgttacac ctccaccaag gaagtgctcg atgctaccct tatccaccag
     7261 agcattactg ggttgtacga aaccaggatc gacctgtccc aactcggcgg agacaagcgc
     7321 cccgccgcca ccaagaaggc tggccaggcc aagaagaaga agtaacccgg gatcgttcaa
     7381 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     7441 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     7501 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     7561 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     7621 atcgctcgaa ttcaaaataa atggtaaaat gtcaaatcaa aactaggctg cagtatgcag
     7681 agcagagtca tgatgatact acttactaca ccgattcttg tgtgcagaaa aatatgttaa
     7741 aataattgaa tctttctcta gccaaatttg acaacaatgt acaccgttca tattgagaga
     7801 cgatgcttct tgtttgcttt cggtggaagc tgcatatact caacattact ccttcagcga
     7861 gttttccaac tgagtcccac attgcccaga cctaacacgg tattcttgtt tataatgaaa
     7921 tgtgccacca catggattga gaccttcgaa gcttctgtgg tctcagtttt agagctagaa
     7981 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     8041 ctttttttgc tagcaggcct taagggccag atcttgggcc cggtacccga tcagattgtc
     8101 gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     8161 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     8221 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc
     8281 cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc
     8341 attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg
     8401 cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat
     8461 ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc
     8521 atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca
     8581 atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc
     8641 atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc ggcttagctc
     8701 atggccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8821 aaaccatcgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgctc acagcccagc
     9121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9361 aaaaacgcca gcaacgcggc ctttcctaag ggttaatgag gtaaagagaa aatgagcaaa
     9421 agcacaaaca cgctaagtgc cggtcgtccg agcgcacgca gcagcaaggc tgcaacgttg
     9481 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     9541 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     9601 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     9661 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     9721 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     9781 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     9841 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg
     9901 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcc
     9961 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
    10021 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
    10081 ggcacccgcg atagtcgcag catcatggac gtggcagtat tccgcctctc gaaacgtgat
    10141 cgccgggcgg gcgaagtcat ccgctacgaa ctgccggacg gtcatgtcga ggtgagcgca
    10201 ggcccggcgg gcatggcctc cgtctgggat tatgacctcg ttctgatggc cgtgtcgcac
    10261 cttacggaaa gcatgaatcg ctaccgggag ggcaagggtg ataagcccgg tcgcgtattc
    10321 cgtccgcatg tggccgacgt tctgaaattc tgtcgccggg cggatggcgg caagcaaaag
    10381 gacgatctcg tcgaaacctg catccgcctg aacacgaccc acgtcgccat gcagcgcacc
    10441 aagaaagcga agaatggccg tcttgtgact gtctccgagg gcgaagccct gatctcgcgc
    10501 tacaagatcg taaagagcga aaccgggcgg cccgagtaca tcgagatcga gcttgctgat
    10561 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
    10621 tactttttga ttgatcccgg catcggtcgt tttctctacc gcctggcacg ccgcgccgca
    10681 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
    10741 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
    10801 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
    10861 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
    10921 gccctagcag gggaaaaagg tcgaaaagct ctctttcctg tggatagcac gtacattggg
    10981 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
    11041 gggaaccggg cacgcatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    11101 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    11161 ggccagcgca cagccgaata gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    11221 cgccccgccg cttcgcgtcg gcctatcgtg gccgctggcc gctcaaaaat ggctggccta
    11281 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccgccgc
    11341 ccacatcaag gcacctcagc ggtgggtatg cctgacgatg cgtgcagacc gaaaccttgc
    11401 gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt
    11461 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
    11521 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
    11581 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
    11641 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
    11701 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
    11761 tcatgcgtga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
    11821 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
    11881 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
    11941 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
    12001 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
    12061 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
    12121 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
    12181 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
    12241 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
    12301 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
    12361 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
    12421 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
    12481 cctctcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
    12541 tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta
    12601 actcaagcgt tagacggacc gcccatgtgg atcactccgt tgccccgtcg ctcaccgtgt
    12661 tggggggaag gtgcacatgg ctcagttctc aatggaaatt atctgcctaa ccggctcagt
    12721 tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg c
//
