LOCUS       D974p7D35s-Cpf1-       12356 bp    DNA     circular SYN 26-AUG-2020
DEFINITION  Ligation of D915p9oNoR-U10-Cpf1-U6At* into D784p7D35s-C9wt-U6GmNhe*
ACCESSION   D974p7D35s-Cpf1-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  D784p7D35s-C9wt-U6GmNhe*
               Fragment ends: XmaI and BamHI
               Fragment size: 8559
            Insert molecule:  D915p9oNoR-U10-Cpf1-U6At*
               Fragment ends: BamHI and XmaI
               Fragment size: 3797
            Sequencing primers:	
            UbiAT-F3	GCTATTGCTTCACCGCCTTA
            NosR2	GAGCGATCTAGTAACATAGATGAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB'"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LB"
                     /SECDescr="Left Border"
     misc_feature    169..584
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             605..838
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    839..1027
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="STLS1"
                     /SECDescr="Intron"
     CDS             1028..1343
                     /gene="Bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1356..1646
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1672..3147
                     /gene="Pro-Ubq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2776..2839
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2842..3145
                     /product="íntron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3168..6950
                     /gene="ORF-1"
                     /translation="MAPKKKRKVGIHGVPAASKLEKFTNCYSLSKTLRFKAIPVGKTQ
                     ENIDNKRLLVEDEKRAEDYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTR
                     TEKENKELENLEINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSF
                     NGFTTAFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEV
                     QEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYIN
                     LYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSI
                     KKLEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAV
                     VTEKYEDDRRKSFKKIGSFSLEQLQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEK
                     LFDADFVLEKSLKKNDAVVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVL
                     AYDILLKVDHIYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYG
                     SKYYLAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNP
                     SEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEKYKDI
                     AGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLHTMYF
                     KLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYD
                     VYKDKRFSEDQYELHIPIAINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLY
                     IVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKE
                     LKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNY
                     MVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLL
                     KTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADYIKKWKLYSYGNRI
                     RIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQSDKAFYSSFMAL
                     MSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIA
                     RKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSVKHKRPAATKKAGQAKKKK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="LbCPf1"
     misc_feature    3174..3194
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    6900..6947
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    6956..7209
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TNOS"
                     /SECDescr="Nos-Terminator"
     misc_feature    7219..7523
                     /gene="U6Gm"
                     /product="U6Gm"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             7524..7550
                     /product="targetRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             7551..7580
                     /product="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    7551..7633
                     /gene="gRNA"
                     /product="gRNA - scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7674..8276
                     /product="Right Border Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7719..7742
                     /gene="RB"
                     /product="RB"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="RB"
                     /SECDescr="RB"
     misc_feature    8290..8948
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8977..10940
                     /gene="''pVS1 ORI'''"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="pVS1"
                     /SECDescr="pVS1 ORI"
     CDS             9303..10478
                     /gene="ParB"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="ParB"
     CDS             11129..12139
                     /gene="strep/spec"
                     /product="Strep/Spec resistence"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Sm/Sp"
                     /SECDescr="Strep/Spec resistence"
     misc_feature    join(12207..12356,1..163)
                     /product="Left Border Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attctagaac atggtggagc
      181 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
      241 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      301 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
      361 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
      421 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      481 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      541 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agtcctccat
      601 aactatgggc cccgagcgca ggcccgccga catccgccgt gccaccgagg ccgacatgcc
      661 agccgtctgc accatcgtga accactacat cgagacttcc acggtcaact tccgcacaga
      721 gccgcaggag cctcaggagt ggaccgacga cctcgtgagg ctgcgcgagc gctacccctg
      781 gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggt
      841 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
      901 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
      961 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     1021 tgtgcaggca cgcaacgcct acgactggac ggcggagagc accgtctacg tgtccccacg
     1081 ccaccagcgc accggcctgg gctccacgct ctacacccac ctgctcaaga gcctggaggc
     1141 ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc aacgacccga gcgtccgtat
     1201 gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg gccgcgggct tcaagcacgg
     1261 caactggcac gacgtgggct tctggcagct cgacttctcc ctgcctgtcc caccccgccc
     1321 ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt atggcattgg gaaaactgtt
     1381 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc
     1441 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat
     1501 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata
     1561 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc
     1621 gaagttaata tgaggagtaa aacactgaag cctgcaggcc ttggcggccg ctttccttac
     1681 attctgagcc tctttccttc taatccactc atctgcatct tcttgtgtcc ttactaatac
     1741 ctcattggtt ccaaattccc tccctttaag caccagctcg tttctgttct tccacagcct
     1801 cccaagtatc caagggacta aagcctccac attcttcaga tcaggatatt cttgtttaag
     1861 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc
     1921 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat
     1981 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa
     2041 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc
     2101 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa
     2161 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat
     2221 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg
     2281 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag
     2341 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag
     2401 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc
     2461 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc
     2521 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca
     2581 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt
     2641 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct
     2701 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa
     2761 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca
     2821 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat
     2881 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta
     2941 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt
     3001 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga
     3061 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat
     3121 taatctgagt ttttctgatt aacaggtact agtggatcct ccaaacaatg gctcccaaga
     3181 agaagcgcaa ggtgggcatc cacggagtcc cagccgcctc caagctggag aagttcacca
     3241 actgctacag cctctctaaa accctccgtt tcaaggcaat tcctgttggt aagactcagg
     3301 agaacatcga caacaagagg cttctcgtgg aggacgaaaa gagagccgag gattacaagg
     3361 gcgtcaagaa gctgctcgac cgctactact tgtccttcat caatgacgtg cttcactcga
     3421 tcaagctcaa gaacctgaac aactacatta gcctcttccg taagaaaacc aggacagaga
     3481 aggagaacaa ggagttggaa aaccttgaga tcaatctccg caaagagatc gccaaggctt
     3541 ttaaggggaa cgagggctat aagtccctgt tcaagaagga catcattgag actatcctac
     3601 cggagttcct cgacgataag gacgaaatcg ccttggtcaa ctcattcaac ggattcacca
     3661 cggcgttcac cggcttcttc gacaaccggg agaatatgtt cagcgaggag gccaagtcca
     3721 ccagtatcgc ttttcgctgc attaacgaga acctgacccg ttacatctcc aacatggaca
     3781 tcttcgaaaa ggttgatgcc atcttcgaca agcacgaggt gcaagagatt aaggagaaga
     3841 tcctcaacag cgactacgac gtggaggact tcttcgaagg agagttcttc aacttcgtcc
     3901 ttacccagga gggcatcgat gtctacaacg ccattatcgg tggcttcgtt accgagtctg
     3961 gagagaagat caaggggctc aatgagtaca tcaacctcta caaccagaaa accaagcaga
     4021 agctgcccaa gtttaagcca ctttacaagc aagtgctttc cgacagggaa agcctgtcgt
     4081 tctacggcga gggctacaca tccgacgagg aggtcttgga ggtgttccgc aacaccctca
     4141 acaagaacag cgaaattttc tcctctatca agaagctgga gaagctcttc aagaacttcg
     4201 acgagtactc aagcgctgga atcttcgtca agaatggtcc cgccatctcc actatttcca
     4261 aggacatctt cggcgagtgg aacgttatca gagataagtg gaacgcagag tatgacgaca
     4321 tccatcttaa gaagaaagcc gtggtcaccg agaagtacga ggacgatcgc cgtaagagct
     4381 tcaagaagat tggatcgttt tccttggaac agctccagga gtacgccgac gctgacctgt
     4441 ctgtggtcga gaagctcaag gagatcatca tacagaaggt agacgaaatc tacaaggtgt
     4501 acggcagctc cgagaagttg ttcgacgccg atttcgtcct tgagaagagc ctcaagaaga
     4561 acgacgcggt tgtggccatt atgaaagacc tgctcgactc cgtcaagtca ttcgagaact
     4621 acatcaaggc tttcttcggg gagggcaagg agactaacag ggacgaatcg ttctacggag
     4681 atttcgtgct tgcctacgac atcttgctca aggttgacca catctacgac gccattcgca
     4741 attacgtcac ccaaaagcct tattccaagg ataagttcaa gctgtacttt cagaacccgc
     4801 agttcatggg cggttgggac aaggacaagg agactgacta ccgagctacc atcctccgtt
     4861 acggcagcaa gtactacctt gccatcatgg acaagaaata cgcaaagtgc ctgcaaaaga
     4921 tcgataagga cgacgtgaac ggaaactacg agaagattaa ctacaagttg ctcccaggcc
     4981 ccaacaagat gctgcctaag gtgttcttct ctaagaagtg gatggcctac tacaatccct
     5041 ccgaggacat ccagaagatt tataagaacg ggaccttcaa gaaaggagat atgttcaacc
     5101 tcaacgactg ccacaagttg atcgacttct tcaaggacag catctcccgc tacccaaagt
     5161 ggagtaacgc ctacgacttc aactttagcg agacagaaaa gtacaaggat atcgctggct
     5221 tctacaggga ggtcgaggag cagggttaca aggtcagctt cgagtctgcc tcgaagaagg
     5281 aggttgacaa gctggtggaa gagggcaagc tctatatgtt ccagatttac aacaaggact
     5341 tctccgacaa gtcccacgga accccgaatc ttcacaccat gtacttcaag ctcttgttcg
     5401 acgagaacaa ccacggccaa atcagacttt caggcggggc ggagctcttc atgcgccgtg
     5461 cctccctgaa gaaagaggaa ctcgtcgttc accccgctaa cagcccaatc gccaacaaga
     5521 accctgataa tcccaagaaa accaccacgt tgtcctacga cgtgtataag gacaagaggt
     5581 tcagcgagga ccagtacgag ctgcacatcc caatcgccat taacaagtgc cccaagaaca
     5641 tctttaagat caacaccgag gtccgcgtgc ttctcaagca tgacgataac ccttacgtca
     5701 tcggaattga ccgtggcgag aggaacctac tctacatcgt ggtcgtggac ggtaagggaa
     5761 atatcgttga gcagtactct ttgaacgaaa tcattaacaa cttcaacggc atccgcatca
     5821 agacggacta ccactccctg ctcgataaga aagagaagga gagattcgag gcacgccaga
     5881 actggacctc gatcgagaat attaaggaac ttaaggctgg ctacatcagc caagtcgtcc
     5941 acaagatttg cgagctggtg gagaagtacg acgccgtgat cgccttggag gacctcaact
     6001 ccgggttcaa gaactcacgg gttaaggtcg agaagcaggt gtaccagaag ttcgagaaga
     6061 tgctcatcga caagctgaac tacatggtgg acaagaaatc caacccgtgc gccaccggag
     6121 gcgctcttaa gggttaccaa atcaccaaca agttcgaatc tttcaagagc atgtccactc
     6181 agaacggctt catcttctac attcccgcct ggctcaccag caagatcgat ccatccaccg
     6241 gatttgtcaa tttgctcaag acaaagtaca cctcgatcgc ggacagcaag aagttcatct
     6301 ccagtttcga ccgtattatg tacgttcccg aggaggacct gttcgagttc gcccttgact
     6361 acaagaactt ctctaggacc gatgctgact acatcaagaa gtggaagttg tactcctacg
     6421 gcaaccgcat ccgcatcttc cgtaacccga agaagaacaa cgtgttcgac tgggaggaag
     6481 tctgcctcac gagcgcctac aaagagctgt tcaataagta cggaattaac tatcagcagg
     6541 gcgacatcag ggccttgctt tgcgagcaat ccgataaggc attttacagc tcattcatgg
     6601 ccctcatgtc cctcatgctc cagatgcgca acagcatcac cgggagaact gacgtggact
     6661 tcctgatctc gcctgtcaag aactccgacg gcattttcta cgactctcgt aactacgagg
     6721 ctcaggagaa cgccatcctt ccaaagaatg ccgatgctaa cggtgcctac aacatcgcga
     6781 ggaaggtttt gtgggccatc ggacagttca agaaggctga ggacgaaaag ctggacaagg
     6841 tcaagattgc catcagcaac aaggagtggt tggagtacgc ccaaacctcc gtgaagcaca
     6901 aacgccccgc agccaccaag aaggctggcc aggccaagaa gaagaagtaa cccgggatcg
     6961 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     7021 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     7081 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     7141 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     7201 actagatcgc tcgaattcaa aataaatggt aaaatgtcaa atcaaaacta ggctgcagta
     7261 tgcagagcag agtcatgatg atactactta ctacaccgat tcttgtgtgc agaaaaatat
     7321 gttaaaataa ttgaatcttt ctctagccaa atttgacaac aatgtacacc gttcatattg
     7381 agagacgatg cttcttgttt gctttcggtg gaagctgcat atactcaaca ttactccttc
     7441 agcgagtttt ccaactgagt cccacattgc ccagacctaa cacggtattc ttgtttataa
     7501 tgaaatgtgc caccacatgg attgagacct tcgaagcttc tgtggtctca gttttagagc
     7561 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     7621 cggtgctttt tttgctagca ggccttaagg gccagatctt gggcccggta cccgatcaga
     7681 ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga caggatatat tggcgggtaa
     7741 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     7801 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gagtgcttgg
     7861 cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc tgacgacgga
     7921 gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg agtgggctgc
     7981 tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa tcctcgatcg
     8041 caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg acgtcctggc
     8101 cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc tggatctcgc
     8161 cttcaatccg ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc aagatatcgc
     8221 ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc gttgatgtgg acgccggctt
     8281 agctcatggc caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8401 caaaaaaacc atcgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
     8701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8941 tatggaaaaa cgccagcaac gcggcctttc ctaagggtta atgaggtaaa gagaaaatga
     9001 gcaaaagcac aaacacgcta agtgccggtc gtccgagcgc acgcagcagc aaggctgcaa
     9061 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     9121 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     9181 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     9241 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     9301 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     9361 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     9421 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     9481 cggcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     9541 aagccgccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     9601 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     9661 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc agtattccgc ctctcgaaac
     9721 gtgatcgccg ggcgggcgaa gtcatccgct acgaactgcc ggacggtcat gtcgaggtga
     9781 gcgcaggccc ggcgggcatg gcctccgtct gggattatga cctcgttctg atggccgtgt
     9841 cgcaccttac ggaaagcatg aatcgctacc gggagggcaa gggtgataag cccggtcgcg
     9901 tattccgtcc gcatgtggcc gacgttctga aattctgtcg ccgggcggat ggcggcaagc
     9961 aaaaggacga tctcgtcgaa acctgcatcc gcctgaacac gacccacgtc gccatgcagc
    10021 gcaccaagaa agcgaagaat ggccgtcttg tgactgtctc cgagggcgaa gccctgatct
    10081 cgcgctacaa gatcgtaaag agcgaaaccg ggcggcccga gtacatcgag atcgagcttg
    10141 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    10201 ccgattactt tttgattgat cccggcatcg gtcgttttct ctaccgcctg gcacgccgcg
    10261 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    10321 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    10381 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    10441 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    10501 aaattgccct agcaggggaa aaaggtcgaa aagctctctt tcctgtggat agcacgtaca
    10561 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    10621 acattgggaa ccgggcacgc atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    10681 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    10741 tgtctggcca gcgcacagcc gaatagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    10801 ccctacgccc cgccgcttcg cgtcggccta tcgtggccgc tggccgctca aaaatggctg
    10861 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    10921 gccgcccaca tcaaggcacc tcagcggtgg gtatgcctga cgatgcgtgc agaccgaaac
    10981 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
    11041 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
    11101 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
    11161 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
    11221 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
    11281 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
    11341 aaacatcatg cgtgaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
    11401 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
    11461 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
    11521 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
    11581 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
    11641 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
    11701 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
    11761 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
    11821 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
    11881 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
    11941 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
    12001 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
    12061 cttggcctct cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
    12121 caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg
    12181 gcttaactca agcgttagac ggaccgccca tgtggatcac tccgttgccc cgtcgctcac
    12241 cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg cctaaccggc
    12301 tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc agcggc
//
