LOCUS       B521p9ioR-35sCas       13780 bp    DNA     circular SYN 04-SEP-2015
DEFINITION  Ligation of B510p7iCas-Gus2* into B358p9ioR-35sCasD10A*
ACCESSION   B521p9ioR-35sCas
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B358p9ioR-35sCasD10A*
               Fragment ends: EcoRI and BamHI
               Fragment size: 9207
            Insert molecule:  B510p7iCas-Gus2*
               Fragment ends: BamHI and EcoRI
               Fragment size: 4573
            Sequencing primers	
            C9	GAACTTCTTAGAAGGCACTTTGTA
            NosF2	GATTAGAGTCCCGCAATTATAC
            STLS      nur Maxi
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             741..777
                     /gene="NptII"
                     /product="Kanamycin resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin resistance"
     misc_feature    778..966
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             967..1733
                     /gene="NptII"
                     /product="Kanamycin-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NptII"
                     /SECDescr="Kanamycin-Resistenz"
     misc_feature    1744..2034
                     /gene="'E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2089..2506
                     /gene="35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             2477..2483
                     /product="TATA-box"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2576..3982
                     /gene="'Cas9"
                     /codon_start=3
                     /translation="PQTRSTALVWPSGPTPSAGPLSPMSTKCLLRSSKSSETLTGTAS
                     RRT"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="'Cas9D10A''"
     misc_feature    2608
                     /gene="D10A"
                     /product="mut to C"
                     /SECDrawAs="Label"
     misc_feature    2955..3047
                     /gene="int"
                     /product="intron from rbcs Nicotiana tobaccum"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3983..6827
                     /gene="'Cas9"
                     /codon_start=3
                     /translation="RRLKRSSLLESLTTSDPWPVATAASPG"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="'Cas9D10A"
     misc_feature    6777..6824
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    6833..7086
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS-T"
                     /SECDescr="Nos-Terminator"
     misc_feature    7096..7540
                     /gene="U6At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             7541..7567
                     /gene="targetRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             7568..7597
                     /gene="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    7568..7650
                     /gene="gRNA"
                     /product="scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7736..7759
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8294..8991
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9279..12066
                     /gene="'pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             12250..13260
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtggccacc atgggcgcct ggattgaaca agatggattg cacgcaggta
      781 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
      841 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
      901 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
      961 gtgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     1141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1441 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtt
     1741 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta
     1801 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt
     1861 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta
     1921 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa
     1981 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc
     2041 tgcaggcatg caagctgatc cactagaggc catggcggcc gcactagatc aacatggtgg
     2101 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
     2161 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     2221 ctatctgtca cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc
     2281 attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg
     2341 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     2401 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     2461 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca ctagttggat
     2521 cctccataac tatggccccc aagaagaagc gcaaagtggg aatccatggc gtcccagccg
     2581 cagacaagaa gtacagcatt ggtctggcca tcgggaccaa ctccgtcggc tgggccgtta
     2641 tcaccgatga gtacaaagtg ccttctaaga agttcaaagt cctcggaaac actgacaggc
     2701 acagcatcaa gaagaacttg attggcgccc ttctcttcga ctccggcgaa accgctgagg
     2761 ccacccgtct gaagcgcacc gccagaaggc gctacacccg tcgcaagaat aggatctgct
     2821 acctccagga gatcttcagc aacgagatgg ccaaagtgga cgattccttc tttcaccgtt
     2881 tggaggagag cttccttgtc gaggaggaca agaagcacga gcgccatccc atcttcggaa
     2941 acattgttga cgaggtaatt tatatacaat gacagtgcaa aaaattttga tacaattaat
     3001 gcatcttaac atgtcatagc taaaaattct attttggtgg aatataggtg gcttaccacg
     3061 agaagtatcc aactatctac cacctcagaa agaagctggt ggactccacc gacaaggccg
     3121 atttgaggct catctacctt gccctcgctc acatgatcaa gttccgcggt cacttcctga
     3181 ttgagggcga cctcaacccc gacaactctg acgtcgataa gttgttcatc caactggtcc
     3241 agacctacaa ccagcttttc gaggagaatc ctatcaacgc ctccggggtt gacgccaagg
     3301 ctattctcag cgcccgtctg tccaagagcc gcaggcttga gaacttgatc gcccagctcc
     3361 caggagagaa gaagaacggc ctcttcggaa acctgatcgc tctctccttg ggccttaccc
     3421 ctaacttcaa gtctaatttt gacctcgccg aggacgccaa gctgcaactc agcaaggaca
     3481 cctacgatga cgacttggac aacctgctcg cccagatcgg cgatcagtac gctgaccttt
     3541 tcttggccgc caagaacctc tccgacgcta ttctgctctc cgacatcctt cgcgtgaaca
     3601 ctgagatcac caaggcccca ctgagcgcct ccatgatcaa gcgttacgac gaacaccacc
     3661 aagatttgac cctcctcaag gctcttgtca gacagcagct gcccgagaag tacaaggaga
     3721 ttttcttcga ccagagcaag aacggatacg ccggttacat cgacggcggg gcctcccaag
     3781 aggagttcta caagttcatc aagcccatct tggagaagat ggacggcacc gaagagctgc
     3841 tcgtgaagct caaccgcgag gatcttttga ggaagcagcg taccttcgac aacggatcta
     3901 ttccccacca gatccatctc ggcgagctgc acgctatcct ccgcaggcaa gaggacttct
     3961 atccattcct taaggacaat cgcgagaaga ttgaaaagat cctcactttt agaatccctt
     4021 actacgtcgg acccttggcc cgtggcaaca gccgcttcgc ctggatgacc aggaagtccg
     4081 aggaaaccat caccccatgg aacttcgagg aggtggttga caagggggcc agcgctcagt
     4141 ccttcattga gcgcatgacc aacttcgata agaacctgcc caacgagaaa gtcctcccaa
     4201 agcactccct tctgtacgag tacttcactg tctacaatga gttgaccaaa gtgaagtacg
     4261 tgaccgaggg tatgcgtaag cctgccttcc tcagcggcga gcagaagaag gccatcgttg
     4321 acctcctgtt caagaccaac aggaaagtca ccgtgaagca gcttaaggag gactacttca
     4381 agaagatcga gtgctttgac tctgtcgaga tcagcggagt ggaggatcgc ttcaacgctt
     4441 ccttgggcac ttaccacgac ctcctgaaga ttatcaagga caaggacttc ctcgacaacg
     4501 aggagaacga agatatcttg gaggacatcg ttcttaccct caccctgttc gaggacagag
     4561 agatgattga ggaacgcctc aagacctacg cccacttgtt cgacgataaa gtcatgaagc
     4621 aacttaagcg taggcgctac accggatggg gcaggctgtc ccgtaagctc atcaacggta
     4681 tccgcgacaa gcagagcggc aagactattc tcgacttctt gaagtccgac ggattcgcca
     4741 atcgcaactt catgcagctg atccacgacg attctcttac cttcaaggag gacatccaaa
     4801 aggctcaagt gtccggccag ggcgacagcc tccacgagca catcgccaac ctggccggga
     4861 gccccgctat taagaaggga atcctccaga ccgtcaaggt ggttgacgag ttggtcaagg
     4921 tgatgggcag acataagcca gagaacatcg tcatcgagat ggccagggaa aaccagacca
     4981 cccagaaggg acagaagaac tcccgtgagc gcatgaagag gattgaggag ggcatcaagg
     5041 agcttggttc ccagatcctc aaggagcacc ccgtggaaaa cacccaactc cagaatgaga
     5101 agctgtattt gtactacctt cagaacgggc gcgatatgta cgttgaccaa gagctggaca
     5161 tcaaccgcct cagcgactac gacgtggatc acattgtccc tcagtccttt ctcaaggacg
     5221 actctatcga caacaaggtg ttgactagaa gcgataagaa ccgcggcaag tccgacaacg
     5281 tcccatccga ggaggtggtc aagaagatga agaattactg gaggcagctg cttaacgcca
     5341 agctcatcac ccagcgcaag ttcgacaact tgaccaaggc cgagcgtgga ggcctcagcg
     5401 aactggacaa ggctggattc atcaagaggc aacttgttga aacccgccag attaccaagc
     5461 acgtggccca gatcctcgac tcccgtatga acactaagta cgatgagaac gacaagctga
     5521 tccgcgaggt caaagtgatt accctcaaga gcaagctcgt gtctgacttc agaaaggact
     5581 tccaattcta caaggttagg gagatcaaca actaccacca cgcccacgat gcttacctta
     5641 atgccgtggt cggcaccgcc ttgatcaaga agtaccccaa gctggagtcc gagttcgtgt
     5701 atggtgacta caaggtctac gacgttcgca agatgatcgc taagagcgag caggagattg
     5761 gcaaggccac cgccaagtac ttcttctact ccaacatcat gaacttcttt aagaccgaga
     5821 tcactttggc taacggggag atccgtaagc gccctctcat tgaaaccaac ggagaaaccg
     5881 gcgagatcgt gtgggacaag ggcagggact tcgccaccgt cagaaaagtg ctgagcatgc
     5941 cccaagtcaa catcgtgaag aaaaccgagg ttcagactgg aggcttctcc aaggagtcta
     6001 tcctcccaaa gcgcaattcc gataagttga ttgcccgtaa gaaggactgg gaccccaaga
     6061 agtacggtgg attcgacagc ccaaccgtcg cctactccgt gcttgtcgtg gctaaagttg
     6121 agaagggcaa gagcaagaag ctcaagtccg tcaaggagct gctcgggatc accatcatgg
     6181 agcgcagctc cttcgagaag aaccctattg atttccttga ggccaagggc tacaaggaag
     6241 tgaagaagga cttgatcatc aagctcccca agtactctct gttcgagctt gagaacggaa
     6301 ggaagcgtat gctcgcctcc gctggcgagc tgcaaaaggg aaacgagttg gccctcccaa
     6361 gcaagtacgt caacttcctg tacctcgcct cccactatga gaagctcaag ggcagccccg
     6421 aggacaacga acagaagcag ttgtttgtgg agcagcataa gcactacctt gacgagatca
     6481 ttgagcagat cagcgagttc tccaagcgcg ttatcctggc tgacgccaat ctcgataaag
     6541 tcctttctgc ctacaacaag cacagggaca agcctatcag agagcaggct gaaaacatta
     6601 tccacttgtt caccctcacc aacctgggtg ccccagccgc cttcaagtac ttcgacacta
     6661 ccatcgaccg caagcgttac acctccacca aggaagtgct cgatgctacc cttatccacc
     6721 agagcattac tgggttgtac gaaaccagga tcgacctgtc ccaactcggc ggagacaagc
     6781 gccccgccgc caccaagaag gctggccagg ccaagaagaa gaagtaaccc gggatcgttc
     6841 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     6901 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     6961 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     7021 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     7081 agatcgctcg aattccttcg ttgaacaacg gaaactcgac ttgccttccg cacaatacat
     7141 catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
     7201 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
     7261 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
     7321 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
     7381 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
     7441 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
     7501 gaagctgagt ttatatacag ctagagtcga agtagtgatt gagacgttcg aagcttctgt
     7561 cgtctcagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     7621 aaaaagtggc accgagtcgg tgcttttttt gtcgacaggc cttaagggcc agatcttggg
     7681 cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag tgtttgacag
     7741 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     7801 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     7861 ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg
     7921 gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag
     7981 aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc
     8041 cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt
     8101 tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc
     8161 gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga
     8221 acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt
     8281 gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8341 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8401 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8461 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8521 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8581 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8641 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8701 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8761 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8821 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8881 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8941 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9001 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9061 tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc tgataccgct
     9121 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     9181 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9241 agtacaatct gctctgatgc cgcatagtta agccagtaac cgtgcggctg catgaaatcc
     9301 tggccggttt gtctgatgcc aagctcgcgg cctggccggc gagcttggcc gctgaagaaa
     9361 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     9421 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     9481 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     9541 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     9601 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     9661 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     9721 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     9781 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     9841 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     9901 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     9961 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
    10021 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
    10081 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
    10141 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
    10201 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
    10261 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
    10321 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
    10381 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
    10441 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
    10501 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
    10561 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggcggc gcaggccgcc
    10621 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
    10681 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    10741 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    10801 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    10861 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    10921 ggcatggcga gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    10981 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    11041 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    11101 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    11161 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    11221 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    11281 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    11341 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    11401 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    11461 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    11521 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    11581 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    11641 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
    11701 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    11761 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    11821 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    11881 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    11941 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    12001 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    12061 ggcaccggtg ggtatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc
    12121 caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg
    12181 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
    12241 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
    12301 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
    12361 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
    12421 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
    12481 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
    12541 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
    12601 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
    12661 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
    12721 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
    12781 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
    12841 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
    12901 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
    12961 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
    13021 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
    13081 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
    13141 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
    13201 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
    13261 tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
    13321 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
    13381 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgaaag gctggctttt
    13441 tcttgttatc gcaatagttg gcgaagtaat cgcaacatag cttgcttggt cgttccgcgt
    13501 gaacgtcggc tcgattgtac ctgcgttcaa atactttgcg atcgtgttgc gcgcctgccc
    13561 ggtgcgtcgg ctgatctcac ggatcgactg cttctctcgc aacgccatcc gacggatgat
    13621 gtttaaaagt cccatgtgga tcactccgtt gccccgtcgc tcaccgtgtt ggggggaagg
    13681 tgcacatggc tcagttctca atggaaatta tctgcctaac cggctcagtt ctgcgtagaa
    13741 accaacatgc aagctccacc gggtgcaaag cggcagcggc
//
