LOCUS       B349p7i-Ucas-U6O       14992 bp    DNA     circular SYN 12-JUL-2018
DEFINITION  Ligation of Frag-BstE-Aar* into B329p7i2xoR-Ucas-U6Os*corrected
            sequence
ACCESSION   B349p7i-Ucas-U6O
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B329p7i2xoR-Ucas-U6Os*
               Fragment ends: AarI and BstEII
               Fragment size: 12207
            Insert molecule:  Frag-BstE-Aar*
               Fragment ends: BstEII and AarI
               Fragment size: 2789
            Primer for gene synthesis
            P7BstE	CGGCCTGAAGCCACACAGTGATA
            P7BsmB	GTGTAGAGCGTGGAGCCCAGTCCCGTCCGCTGGTGGCGGGGGGAcACGTACACGGTGGAC
            P7Aar	ccagggacttcagcaggtggGTGTAGAGCGTGGAGCC
            Sequencing primers	
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
            Bar-R	GTGGTTGACGATGGTGCAGA
            aadAend	GTGAAAGGCGAGATCACCA
            LH3	CATCATCCGTCGGATGGCGT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    315..1099
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1113..1346
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1347..1535
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1536..1853
                     /gene="bar'"
                     /product="basta resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1864..2154
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2209..3717
                     /gene="Ubi-int"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3196..3715
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3743..8031
                     /gene="'Cas9"
                     /translation="PDPRRSAKWESTELQTRSTALVWPSGPTPSAGPLSPMSTKCLLR
                     SSKSSETLTGTASRRT"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="'Cas9D10A"
     misc_feature    3748..3768
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3812
                     /gene="D10A"
                     /product="mut to C"
                     /SECDrawAs="Label"
     misc_feature    4159..4251
                     /gene="int"
                     /product="intron from rbcs Nicotiana tobaccum"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7981..8028
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8037..8290
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS-T"
                     /SECDescr="Nos-Terminator"
     misc_feature    8300..8752
                     /gene="PU6C4"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8754..8779
                     /gene="MCS"
                     /codon_start=2
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             8780..8809
                     /gene="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    8780..8862
                     /gene="gRNA"
                     /product="scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8948..8971
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9506..10203
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    10491..13278
                     /gene="'pVS1'"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             13462..14472
                     /gene="Sm/Sp'"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc gggccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      361 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      421 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      481 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      541 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      601 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac
      661 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
      721 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      781 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
      841 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      901 cacgaggagc atcgtggaaa aagaagacct tccaaccacg tcttcaaagc aagtggattg
      961 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     1021 ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct
     1081 ctctacaaat ctatctctct cgagtggcca ccatgggccc agaacgacgc ccggccgaca
     1141 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     1201 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     1261 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     1321 gcatcgccta cgcgggcccc tggaaggtaa gtttctgctt ctacctttga tatatatata
     1381 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     1441 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     1501 tttctaatat atgaccaaaa tttgttgatg tgcaggcacg caacgcctac gactggacgg
     1561 ccgagtccac cgtgtacgtg tccccccgcc accagcggac gggactgggc tccacgctct
     1621 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
     1681 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
     1741 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
     1801 acttcagcct gccggtacag gcccgtccgg tcctgcccgt caccgagatc tgaacgcgtt
     1861 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta
     1921 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt
     1981 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta
     2041 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa
     2101 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc
     2161 tgcaggcatg caagctgatc cactagaggc catggcggcc gcactagtct gcagtgcagc
     2221 gtgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa
     2281 ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata
     2341 tatttaaact ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt
     2401 agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa
     2461 caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag
     2521 cttcacctat ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa
     2581 tggtttttat agactaattt ttttagtaca tctattttat tctattttag cctctaaatt
     2641 aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata
     2701 aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac
     2761 atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgatc gacgagtcta
     2821 acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg
     2881 catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc
     2941 cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg
     3001 gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc
     3061 gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa
     3121 cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg
     3181 cacctccgct tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag
     3241 atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta
     3301 gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt
     3361 cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc
     3421 tagccgttcc gcagacggga tcgatctagg ataggtatac atgttgatgt gggttttact
     3481 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     3541 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     3601 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     3661 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggtact
     3721 agatcctcca ccgaaatggc ctccggaccc aagaagaagc gcaaagtggg aatccacgga
     3781 gctccagaca agaagtacag cattggtctg gccatcggga ccaactccgt cggctgggcc
     3841 gttatcaccg atgagtacaa agtgccttct aagaagttca aagtcctcgg aaacactgac
     3901 aggcacagca tcaagaagaa cttgattggc gcccttctct tcgactccgg cgaaaccgct
     3961 gaggccaccc gtctgaagcg caccgccaga aggcgctaca cccgtcgcaa gaataggatc
     4021 tgctacctcc aggagatctt cagcaacgag atggccaaag tggacgattc cttctttcac
     4081 cgtttggagg agagcttcct tgtcgaggag gacaagaagc acgagcgcca tcccatcttc
     4141 ggaaacattg ttgacgaggt aatttatata caatgacagt gcaaaaaatt ttgatacaat
     4201 taatgcatct taacatgtca tagctaaaaa ttctattttg gtggaatata ggtggcttac
     4261 cacgagaagt atccaactat ctaccacctc agaaagaagc tggtggactc caccgacaag
     4321 gccgatttga ggctcatcta ccttgccctc gctcacatga tcaagttccg cggtcacttc
     4381 ctgattgagg gcgacctcaa ccccgacaac tctgacgtcg ataagttgtt catccaactg
     4441 gtccagacct acaaccagct tttcgaggag aatcctatca acgcctccgg ggttgacgcc
     4501 aaggctattc tcagcgcccg tctgtccaag agccgcaggc ttgagaactt gatcgcccag
     4561 ctcccaggag agaagaagaa cggcctcttc ggaaacctga tcgctctctc cttgggcctt
     4621 acccctaact tcaagtctaa ttttgacctc gccgaggacg ccaagctgca actcagcaag
     4681 gacacctacg atgacgactt ggacaacctg ctcgcccaga tcggcgatca gtacgctgac
     4741 cttttcttgg ccgccaagaa cctctccgac gctattctgc tctccgacat ccttcgcgtg
     4801 aacactgaga tcaccaaggc cccactgagc gcctccatga tcaagcgtta cgacgaacac
     4861 caccaagatt tgaccctcct caaggctctt gtcagacagc agctgcccga gaagtacaag
     4921 gagattttct tcgaccagag caagaacgga tacgccggtt acatcgacgg cggggcctcc
     4981 caagaggagt tctacaagtt catcaagccc atcttggaga agatggacgg caccgaagag
     5041 ctgctcgtga agctcaaccg cgaggatctt ttgaggaagc agcgtacctt cgacaacgga
     5101 tctattcccc accagatcca tctcggcgag ctgcacgcta tcctccgcag gcaagaggac
     5161 ttctatccat tccttaagga caatcgcgag aagattgaaa agatcctcac ttttagaatc
     5221 ccttactacg tcggaccctt ggcccgtggc aacagccgct tcgcctggat gaccaggaag
     5281 tccgaggaaa ccatcacccc atggaacttc gaggaggtgg ttgacaaggg ggccagcgct
     5341 cagtccttca ttgagcgcat gaccaacttc gataagaacc tgcccaacga gaaagtcctc
     5401 ccaaagcact cccttctgta cgagtacttc actgtctaca atgagttgac caaagtgaag
     5461 tacgtgaccg agggtatgcg taagcctgcc ttcctcagcg gcgagcagaa gaaggccatc
     5521 gttgacctcc tgttcaagac caacaggaaa gtcaccgtga agcagcttaa ggaggactac
     5581 ttcaagaaga tcgagtgctt tgactctgtc gagatcagcg gagtggagga tcgcttcaac
     5641 gcttccttgg gcacttacca cgacctcctg aagattatca aggacaagga cttcctcgac
     5701 aacgaggaga acgaagatat cttggaggac atcgttctta ccctcaccct gttcgaggac
     5761 agagagatga ttgaggaacg cctcaagacc tacgcccact tgttcgacga taaagtcatg
     5821 aagcaactta agcgtaggcg ctacaccgga tggggcaggc tgtcccgtaa gctcatcaac
     5881 ggtatccgcg acaagcagag cggcaagact attctcgact tcttgaagtc cgacggattc
     5941 gccaatcgca acttcatgca gctgatccac gacgattctc ttaccttcaa ggaggacatc
     6001 caaaaggctc aagtgtccgg ccagggcgac agcctccacg agcacatcgc caacctggcc
     6061 gggagccccg ctattaagaa gggaatcctc cagaccgtca aggtggttga cgagttggtc
     6121 aaggtgatgg gcagacataa gccagagaac atcgtcatcg agatggccag ggaaaaccag
     6181 accacccaga agggacagaa gaactcccgt gagcgcatga agaggattga ggagggcatc
     6241 aaggagcttg gttcccagat cctcaaggag caccccgtgg aaaacaccca actccagaat
     6301 gagaagctgt atttgtacta ccttcagaac gggcgcgata tgtacgttga ccaagagctg
     6361 gacatcaacc gcctcagcga ctacgacgtg gatcacattg tccctcagtc ctttctcaag
     6421 gacgactcta tcgacaacaa ggtgttgact agaagcgata agaaccgcgg caagtccgac
     6481 aacgtcccat ccgaggaggt ggtcaagaag atgaagaatt actggaggca gctgcttaac
     6541 gccaagctca tcacccagcg caagttcgac aacttgacca aggccgagcg tggaggcctc
     6601 agcgaactgg acaaggctgg attcatcaag aggcaacttg ttgaaacccg ccagattacc
     6661 aagcacgtgg cccagatcct cgactcccgt atgaacacta agtacgatga gaacgacaag
     6721 ctgatccgcg aggtcaaagt gattaccctc aagagcaagc tcgtgtctga cttcagaaag
     6781 gacttccaat tctacaaggt tagggagatc aacaactacc accacgccca cgatgcttac
     6841 cttaatgccg tggtcggcac cgccttgatc aagaagtacc ccaagctgga gtccgagttc
     6901 gtgtatggtg actacaaggt ctacgacgtt cgcaagatga tcgctaagag cgagcaggag
     6961 attggcaagg ccaccgccaa gtacttcttc tactccaaca tcatgaactt ctttaagacc
     7021 gagatcactt tggctaacgg ggagatccgt aagcgccctc tcattgaaac caacggagaa
     7081 accggcgaga tcgtgtggga caagggcagg gacttcgcca ccgtcagaaa agtgctgagc
     7141 atgccccaag tcaacatcgt gaagaaaacc gaggttcaga ctggaggctt ctccaaggag
     7201 tctatcctcc caaagcgcaa ttccgataag ttgattgccc gtaagaagga ctgggacccc
     7261 aagaagtacg gtggattcga cagcccaacc gtcgcctact ccgtgcttgt cgtggctaaa
     7321 gttgagaagg gcaagagcaa gaagctcaag tccgtcaagg agctgctcgg gatcaccatc
     7381 atggagcgca gctccttcga gaagaaccct attgatttcc ttgaggccaa gggctacaag
     7441 gaagtgaaga aggacttgat catcaagctc cccaagtact ctctgttcga gcttgagaac
     7501 ggaaggaagc gtatgctcgc ctccgctggc gagctgcaaa agggaaacga gttggccctc
     7561 ccaagcaagt acgtcaactt cctgtacctc gcctcccact atgagaagct caagggcagc
     7621 cccgaggaca acgaacagaa gcagttgttt gtggagcagc ataagcacta ccttgacgag
     7681 atcattgagc agatcagcga gttctccaag cgcgttatcc tggctgacgc caatctcgat
     7741 aaagtccttt ctgcctacaa caagcacagg gacaagccta tcagagagca ggctgaaaac
     7801 attatccact tgttcaccct caccaacctg ggtgccccag ccgccttcaa gtacttcgac
     7861 actaccatcg accgcaagcg ttacacctcc accaaggaag tgctcgatgc tacccttatc
     7921 caccagagca ttactgggtt gtacgaaacc aggatcgacc tgtcccaact cggcggagac
     7981 aagcgccccg ccgccaccaa gaaggctggc caggccaaga agaagaagta acccgggatc
     8041 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     8101 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     8161 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     8221 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     8281 tactagatcg ctcgaattcg tacagcatta cgtaggtacg ttttcttttt cttcccggag
     8341 agatgatacg ataatcatgt aaacccagaa tttaaaaaat attctttact ataaaaattt
     8401 taattaggga acgtattatt ttttacatga caccttttga gaaagaggga cttgtaatat
     8461 gggacaaatg aacaatttct aagaaatggg catatgactc tcagtacaat ggaccaaatt
     8521 ccctccagtc ggcccagcaa tacaaaggga aagaaatgag ggggcccaca ggccacggcc
     8581 cacttttctc cgtggtgggg agatccagct agaggtccgg cccacaagtg gcccttgccc
     8641 cgtgggacgg tgggattgca gagcgcgtgg gcggaaacaa cagtttagta ccacctcgct
     8701 cacgcaacga cgcgaccact tgcttataag ctgctgcgct gaggctcagc ttgagacgtt
     8761 cgaagcttct gtcgtctcag ttttagagct agaaatagca agttaaaata aggctagtcc
     8821 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgtcgacag gccttaaggg
     8881 ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc
     8941 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     9001 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     9061 accacagggt tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc
     9121 acccaaacgt cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc
     9181 tgggtcggct agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac
     9241 gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg
     9301 cttgtcgctc tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg
     9361 atgatctggc gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac
     9421 gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc
     9481 gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg aagatccttt ttgataatct
     9541 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9601 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9661 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9721 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9781 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9841 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9901 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9961 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10021 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10081 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10141 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10201 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10261 catgttcttt cctgcgttat cccctgattc tgtggataac cgattaccgc ctttgagtga
    10321 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10381 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
    10441 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta accgtgcggc
    10501 tgcatgaaat cctggccggt ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg
    10561 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
    10621 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
    10681 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
    10741 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
    10801 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
    10861 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
    10921 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
    10981 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
    11041 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
    11101 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
    11161 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
    11221 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
    11281 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
    11341 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
    11401 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
    11461 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
    11521 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
    11581 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
    11641 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
    11701 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
    11761 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg
    11821 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
    11881 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
    11941 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
    12001 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
    12061 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
    12121 gcagggccgg ccggcatggc gagtgtgtgg gattacgacc tggtactgat ggcggtttcc
    12181 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
    12241 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
    12301 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
    12361 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
    12421 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
    12481 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
    12541 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
    12601 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
    12661 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
    12721 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
    12781 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
    12841 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
    12901 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
    12961 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
    13021 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
    13081 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
    13141 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
    13201 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
    13261 cgcccacatc aaggcaccgg tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc
    13321 tcgttcgcca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga
    13381 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
    13441 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
    13501 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
    13561 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
    13621 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
    13681 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
    13741 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
    13801 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
    13861 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
    13921 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
    13981 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
    14041 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
    14101 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
    14161 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
    14221 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
    14281 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
    14341 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
    14401 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
    14461 gtcggcaaat aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac
    14521 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
    14581 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa
    14641 aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat agcttgcttg
    14701 gtcgttccgc gtgaacgtcg gctcgattgt acctgcgttc aaatactttg cgatcgtgtt
    14761 gcgcgcctgc ccggtgcgtc ggctgatctc acggatcgac tgcttctctc gcaacgccat
    14821 ccgacggatg atgtttaaaa gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg
    14881 ttggggggaa ggtgcacatg gctcagttct caatggaaat tatctgccta accggctcag
    14941 ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa agcggcagcg gc
//
