LOCUS       C034p7ioR-35sCas       13433 bp    DNA     circular SYN 14-JUL-2016
DEFINITION  Ligation of B414p7o-LH* into B792p6ioR-35sCasWToi*
ACCESSION   C034p7ioR-35sCas
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B792p6ioR-35sCasWToi*
               Fragment ends: MluI and XhoI
               Fragment size: 12674
            Insert molecule:  B414p7o-LH*
               Fragment ends: XhoI and MluI
               Fragment size: 759
            Sequencing primers:
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..978
                     /gene="Bar'"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    979..1167
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="STLS1"
                     /SECDescr="Intron"
     CDS             1168..1483
                     /gene="''Bar'"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1496..1786
                     /gene="'E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1841..2258
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             2229..2235
                     /product="TATA-box"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2284..6480
                     /gene="'Cas9WT"
                     /translation="MAPKKKRKVGIHGAPDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST
                     DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS
                     MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP
                     ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN
                     REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER
                     MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
                     FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE
                     ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN
                     GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN
                     LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI
                     EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV
                     PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
                     TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL
                     KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
                     DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW
                     DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG
                     GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV
                     KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS
                     PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA
                     ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ
                     LGGDKRPAATKKAGQAKKKK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Cas9WToi"
                     /SECDescr="no intronsequence"
     misc_feature    2290..2310
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    6430..6477
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    6486..6739
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS-T"
                     /SECDescr="Nos-Terminator"
     misc_feature    6744..7304
                     /gene="'RNA'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             6749..7193
                     /gene="U6At"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             7194..7220
                     /gene="targetRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             7221..7250
                     /gene="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    7221..7303
                     /gene="gRNA"
                     /product="scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7389..7412
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    7947..8644
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8932..11719
                     /gene="'pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             11903..12913
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt
      781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc
      841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg
      901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc
      961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc
     1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac
     1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc
     1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg
     1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc
     1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt
     1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt
     1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat
     1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt
     1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca
     1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca
     1801 tgcaagctga tccactagag gccatggcgg ccgcactaga tcaacatggt ggagcacgac
     1861 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag
     1921 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     1981 cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat
     2041 aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca
     2101 cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     2161 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     2221 ccttcctcta tataaggaag ttcatttcat ttggagagga cactagttgg atcctccata
     2281 actatggccc ccaagaagaa gcgcaaagtg ggaatccacg gagctccaga caagaagtac
     2341 agcattggtc tggacatcgg gaccaactcc gtcggctggg ccgttatcac cgatgagtac
     2401 aaagtgcctt ctaagaagtt caaagtcctc ggaaacactg acaggcacag catcaagaag
     2461 aacttgattg gcgcccttct cttcgactcc ggcgaaaccg ctgaggccac ccgtctgaag
     2521 cgcaccgcca gaaggcgcta cacccgtcgc aagaatagga tctgctacct ccaggagatc
     2581 ttcagcaacg agatggccaa agtggacgat tccttctttc accgtttgga ggagagcttc
     2641 cttgtcgagg aggacaagaa gcacgagcgc catcccatct tcggaaacat tgttgacgag
     2701 gtggcttacc acgagaagta tccaactatc taccacctca gaaagaagct ggtggactcc
     2761 accgacaagg ccgatttgag gctcatctac cttgccctcg ctcacatgat caagttccgc
     2821 ggtcacttcc tgattgaggg cgacctcaac cccgacaact ctgacgtcga taagttgttc
     2881 atccaactgg tccagaccta caaccagctt ttcgaggaga atcctatcaa cgcctccggg
     2941 gttgacgcca aggctattct cagcgcccgt ctgtccaaga gccgcaggct tgagaacttg
     3001 atcgcccagc tcccaggaga gaagaagaac ggcctcttcg gaaacctgat cgctctctcc
     3061 ttgggcctta cccctaactt caagtctaat tttgacctcg ccgaggacgc caagctgcaa
     3121 ctcagcaagg acacctacga tgacgacttg gacaacctgc tcgcccagat cggcgatcag
     3181 tacgctgacc ttttcttggc cgccaagaac ctctccgacg ctattctgct ctccgacatc
     3241 cttcgcgtga acactgagat caccaaggcc ccactgagcg cctccatgat caagcgttac
     3301 gacgaacacc accaagattt gaccctcctc aaggctcttg tcagacagca gctgcccgag
     3361 aagtacaagg agattttctt cgaccagagc aagaacggat acgccggtta catcgacggc
     3421 ggggcctccc aagaggagtt ctacaagttc atcaagccca tcttggagaa gatggacggc
     3481 accgaagagc tgctcgtgaa gctcaaccgc gaggatcttt tgaggaagca gcgtaccttc
     3541 gacaacggat ctattcccca ccagatccat ctcggcgagc tgcacgctat cctccgcagg
     3601 caagaggact tctatccatt ccttaaggac aatcgcgaga agattgaaaa gatcctcact
     3661 tttagaatcc cttactacgt cggacccttg gcccgtggca acagccgctt cgcctggatg
     3721 accaggaagt ccgaggaaac catcacccca tggaacttcg aggaggtggt tgacaagggg
     3781 gccagcgctc agtccttcat tgagcgcatg accaacttcg ataagaacct gcccaacgag
     3841 aaagtcctcc caaagcactc ccttctgtac gagtacttca ctgtctacaa tgagttgacc
     3901 aaagtgaagt acgtgaccga gggtatgcgt aagcctgcct tcctcagcgg cgagcagaag
     3961 aaggccatcg ttgacctcct gttcaagacc aacaggaaag tcaccgtgaa gcagcttaag
     4021 gaggactact tcaagaagat cgagtgcttt gactctgtcg agatcagcgg agtggaggat
     4081 cgcttcaacg cttccttggg cacttaccac gacctcctga agattatcaa ggacaaggac
     4141 ttcctcgaca acgaggagaa cgaagatatc ttggaggaca tcgttcttac cctcaccctg
     4201 ttcgaggaca gagagatgat tgaggaacgc ctcaagacct acgcccactt gttcgacgat
     4261 aaagtcatga agcaacttaa gcgtaggcgc tacaccggat ggggcaggct gtcccgtaag
     4321 ctcatcaacg gtatccgcga caagcagagc ggcaagacta ttctcgactt cttgaagtcc
     4381 gacggattcg ccaatcgcaa cttcatgcag ctgatccacg acgattctct taccttcaag
     4441 gaggacatcc aaaaggctca agtgtccggc cagggcgaca gcctccacga gcacatcgcc
     4501 aacctggccg ggagccccgc tattaagaag ggaatcctcc agaccgtcaa ggtggttgac
     4561 gagttggtca aggtgatggg cagacataag ccagagaaca tcgtcatcga gatggccagg
     4621 gaaaaccaga ccacccagaa gggacagaag aactcccgtg agcgcatgaa gaggattgag
     4681 gagggcatca aggagcttgg ttcccagatc ctcaaggagc accccgtgga aaacacccaa
     4741 ctccagaatg agaagctgta tttgtactac cttcagaacg ggcgcgatat gtacgttgac
     4801 caagagctgg acatcaaccg cctcagcgac tacgacgtgg atcacattgt ccctcagtcc
     4861 tttctcaagg acgactctat cgacaacaag gtgttgacta gaagcgataa gaaccgcggc
     4921 aagtccgaca acgtcccatc cgaggaggtg gtcaagaaga tgaagaatta ctggaggcag
     4981 ctgcttaacg ccaagctcat cacccagcgc aagttcgaca acttgaccaa ggccgagcgt
     5041 ggaggcctca gcgaactgga caaggctgga ttcatcaaga ggcaacttgt tgaaacccgc
     5101 cagattacca agcacgtggc ccagatcctc gactcccgta tgaacactaa gtacgatgag
     5161 aacgacaagc tgatccgcga ggtcaaagtg attaccctca agagcaagct cgtgtctgac
     5221 ttcagaaagg acttccaatt ctacaaggtt agggagatca acaactacca ccacgcccac
     5281 gatgcttacc ttaatgccgt ggtcggcacc gccttgatca agaagtaccc caagctggag
     5341 tccgagttcg tgtatggtga ctacaaggtc tacgacgttc gcaagatgat cgctaagagc
     5401 gagcaggaga ttggcaaggc caccgccaag tacttcttct actccaacat catgaacttc
     5461 tttaagaccg agatcacttt ggctaacggg gagatccgta agcgccctct cattgaaacc
     5521 aacggagaaa ccggcgagat cgtgtgggac aagggcaggg acttcgccac cgtcagaaaa
     5581 gtgctgagca tgccccaagt caacatcgtg aagaaaaccg aggttcagac tggaggcttc
     5641 tccaaggagt ctatcctccc aaagcgcaat tccgataagt tgattgcccg taagaaggac
     5701 tgggacccca agaagtacgg tggattcgac agcccaaccg tcgcctactc cgtgcttgtc
     5761 gtggctaaag ttgagaaggg caagagcaag aagctcaagt ccgtcaagga gctgctcggg
     5821 atcaccatca tggagcgcag ctccttcgag aagaacccta ttgatttcct tgaggccaag
     5881 ggctacaagg aagtgaagaa ggacttgatc atcaagctcc ccaagtactc tctgttcgag
     5941 cttgagaacg gaaggaagcg tatgctcgcc tccgctggcg agctgcaaaa gggaaacgag
     6001 ttggccctcc caagcaagta cgtcaacttc ctgtacctcg cctcccacta tgagaagctc
     6061 aagggcagcc ccgaggacaa cgaacagaag cagttgtttg tggagcagca taagcactac
     6121 cttgacgaga tcattgagca gatcagcgag ttctccaagc gcgttatcct ggctgacgcc
     6181 aatctcgata aagtcctttc tgcctacaac aagcacaggg acaagcctat cagagagcag
     6241 gctgaaaaca ttatccactt gttcaccctc accaacctgg gtgccccagc cgccttcaag
     6301 tacttcgaca ctaccatcga ccgcaagcgt tacacctcca ccaaggaagt gctcgatgct
     6361 acccttatcc accagagcat tactgggttg tacgaaacca ggatcgacct gtcccaactc
     6421 ggcggagaca agcgccccgc cgccaccaag aaggctggcc aggccaagaa gaagaagtaa
     6481 cccgggatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     6541 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     6601 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     6661 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     6721 catctatgtt actagatcgc tcgaattcct tcgttgaaca acggaaactc gacttgcctt
     6781 ccgcacaata catcatttct tcttagcttt ttttcttctt cttcgttcat acagtttttt
     6841 tttgtttatc agcttacatt ttcttgaacc gtagctttcg ttttcttctt tttaactttc
     6901 cattcggagt ttttgtatct tgtttcatag tttgtcccag gattagaatg attaggcatc
     6961 gaaccttcaa gaatttgatt gaataaaaca tcttcattct taagatatga agataatctt
     7021 caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg gaaagaacaa
     7081 tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct
     7141 tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg attgagacgt
     7201 tcgaagcttc tgtcgtctca gttttagagc tagaaatagc aagttaaaat aaggctagtc
     7261 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgtcgaca ggccttaagg
     7321 gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat
     7381 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     7441 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     7501 aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac
     7561 cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt
     7621 ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga
     7681 cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct
     7741 gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag
     7801 gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca
     7861 cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc
     7921 cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt tttgataatc
     7981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg cctttgagtg
     8761 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8821 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8881 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt aaccgtgcgg
     8941 ctgcatgaaa tcctggccgg tttgtctgat gccaagctcg cggcctggcc ggcgagcttg
     9001 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     9061 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
     9121 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     9181 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     9241 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     9301 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     9361 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     9421 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     9481 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
     9541 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
     9601 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
     9661 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
     9721 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
     9781 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
     9841 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
     9901 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
     9961 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
    10021 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
    10081 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc
    10141 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc
    10201 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
    10261 ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
    10321 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
    10381 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
    10441 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
    10501 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
    10561 cgcagggccg gccggcatgg cgagtgtgtg ggattacgac ctggtactga tggcggtttc
    10621 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
    10681 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
    10741 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
    10801 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
    10861 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
    10921 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
    10981 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
    11041 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
    11101 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
    11161 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
    11221 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
    11281 aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat
    11341 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
    11401 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
    11461 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
    11521 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
    11581 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
    11641 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
    11701 gcgcccacat caaggcaccg gtgggtatgc ctgacgatgc gtggagaccg aaaccttgcg
    11761 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
    11821 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
    11881 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
    11941 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
    12001 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
    12061 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
    12121 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
    12181 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
    12241 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
    12301 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
    12361 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
    12421 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
    12481 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
    12541 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
    12601 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
    12661 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
    12721 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
    12781 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
    12841 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
    12901 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
    12961 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
    13021 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatga
    13081 aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca tagcttgctt
    13141 ggtcgttccg cgtgaacgtc ggctcgattg tacctgcgtt caaatacttt gcgatcgtgt
    13201 tgcgcgcctg cccggtgcgt cggctgatct cacggatcga ctgcttctct cgcaacgcca
    13261 tccgacggat gatgtttaaa agtcccatgt ggatcactcc gttgccccgt cgctcaccgt
    13321 gttgggggga aggtgcacat ggctcagttc tcaatggaaa ttatctgcct aaccggctca
    13381 gttctgcgta gaaaccaaca tgcaagctcc accgggtgca aagcggcagc ggc
//
