LOCUS       B350p7i-UcasW-U6       14996 bp    DNA     circular SYN 05-OCT-2016
DEFINITION  Ligation of Frag-BstE-Aar* into B331p7i2xoR-UcasW-U6Os4*
ACCESSION   B350p7i-UcasW-U6
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 14996)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B331p7i2xoR-UcasW-U6Os4*
               Fragment ends: AarI and BstEII
               Fragment size: 12207
            Insert molecule:  Frag-BstE-Aar*
               Fragment ends: BstEII and AarI
               Fragment size: 2789
            Primer for gene synthesis
            P7BstE	CGGCCTGAAGCCACACAGTGATA
            P7BsmB	GTGTAGAGCGTGGAGCCCAGTCCCGTCCGCTGGTGGCGGGGGGAcACGTACACGGTGGAC
            P7Aar	ccagggacttcagcaggtggGTGTAGAGCGTGGAGCC
            Sequencing primers	
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
            Bar-R	GTGGTTGACGATGGTGCAGA
            aadAend	GTGAAAGGCGAGATCACCA
            LH3	CATCATCCGTCGGATGGCGT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    315..1099
                     /gene="P2x35s'"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1113..1346
                     /gene="bar"
                     /product="Basta-Resistenz"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1347..1535
                     /gene="STLS1"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1536..1853
                     /gene="bar'"
                     /product="basta resistance"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1868..2158
                     /gene="E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2213..3721
                     /gene="Ubi-int"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3200..3719
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             3740..4162
                     /gene="Cas9WT"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3752..3772
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3816
                     /gene="mut"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4163..4255
                     /gene="int"
                     /product="intron from rbcs Nicotiana tobaccum"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             4256..8035
                     /gene="Cas9WT"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Cas9WT"
     misc_feature    7985..8032
                     /product="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8041..8294
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS-T"
                     /SECDescr="Nos-Terminator"
     misc_feature    8304..8756
                     /gene="U6Os"
                     /product="U6Os C4"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8768..8783
                     /product="targetRNA"
                     /codon_start=2
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             8784..8813
                     /product="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    8784..8866
                     /product="gRNA scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    8952..8975
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9510..10207
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    10495..13282
                     /gene="'pVS1'"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             13466..14476
                     /gene="Sm/Sp'"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc gggccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      361 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      421 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      481 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      541 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      601 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac
      661 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
      721 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
      781 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
      841 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      901 cacgaggagc atcgtggaaa aagaagacct tccaaccacg tcttcaaagc aagtggattg
      961 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     1021 ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct
     1081 ctctacaaat ctatctctct cgagtggcca ccatgggccc agaacgacgc ccggccgaca
     1141 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     1201 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     1261 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     1321 gcatcgccta cgcgggcccc tggaaggtaa gtttctgctt ctacctttga tatatatata
     1381 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     1441 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     1501 tttctaatat atgaccaaaa tttgttgatg tgcaggcacg caacgcctac gactggacgg
     1561 ccgagtccac cgtgtacgtg tccccccgcc accagcggac gggactgggc tccacgctct
     1621 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
     1681 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
     1741 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
     1801 acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatc tgagatcacg
     1861 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     1921 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     1981 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     2041 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     2101 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga
     2161 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtctgcagtg
     2221 cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa
     2281 aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata
     2341 catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg
     2401 ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg
     2461 acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa
     2521 atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg
     2581 ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta
     2641 aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag
     2701 aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg
     2761 aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gatcgacgag
     2821 tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc
     2881 acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt
     2941 gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca
     3001 ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc
     3061 cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc
     3121 ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg
     3181 tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc tctaccttct
     3241 ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt
     3301 gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt
     3361 acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg
     3421 gctctagccg ttccgcagac gggatcgatc taggataggt atacatgttg atgtgggttt
     3481 tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta
     3541 cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat
     3601 gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat
     3661 ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcagg
     3721 tactagatcc tccaccgaaa tggcctccgg acccaagaag aagcgcaaag tgggaatcca
     3781 cggagctcca gacaagaagt acagcattgg tctggacatc gggaccaact ccgtcggctg
     3841 ggccgttatc accgatgagt acaaagtgcc ttctaagaag ttcaaagtcc tcggaaacac
     3901 tgacaggcac agcatcaaga agaacttgat tggcgccctt ctcttcgact ccggcgaaac
     3961 cgctgaggcc acccgtctga agcgcaccgc cagaaggcgc tacacccgtc gcaagaatag
     4021 gatctgctac ctccaggaga tcttcagcaa cgagatggcc aaagtggacg attccttctt
     4081 tcaccgtttg gaggagagct tccttgtcga ggaggacaag aagcacgagc gccatcccat
     4141 cttcggaaac attgttgacg aggtaattta tatacaatga cagtgcaaaa aattttgata
     4201 caattaatgc atcttaacat gtcatagcta aaaattctat tttggtggaa tataggtggc
     4261 ttaccacgag aagtatccaa ctatctacca cctcagaaag aagctggtgg actccaccga
     4321 caaggccgat ttgaggctca tctaccttgc cctcgctcac atgatcaagt tccgcggtca
     4381 cttcctgatt gagggcgacc tcaaccccga caactctgac gtcgataagt tgttcatcca
     4441 actggtccag acctacaacc agcttttcga ggagaatcct atcaacgcct ccggggttga
     4501 cgccaaggct attctcagcg cccgtctgtc caagagccgc aggcttgaga acttgatcgc
     4561 ccagctccca ggagagaaga agaacggcct cttcggaaac ctgatcgctc tctccttggg
     4621 ccttacccct aacttcaagt ctaattttga cctcgccgag gacgccaagc tgcaactcag
     4681 caaggacacc tacgatgacg acttggacaa cctgctcgcc cagatcggcg atcagtacgc
     4741 tgaccttttc ttggccgcca agaacctctc cgacgctatt ctgctctccg acatccttcg
     4801 cgtgaacact gagatcacca aggccccact gagcgcctcc atgatcaagc gttacgacga
     4861 acaccaccaa gatttgaccc tcctcaaggc tcttgtcaga cagcagctgc ccgagaagta
     4921 caaggagatt ttcttcgacc agagcaagaa cggatacgcc ggttacatcg acggcggggc
     4981 ctcccaagag gagttctaca agttcatcaa gcccatcttg gagaagatgg acggcaccga
     5041 agagctgctc gtgaagctca accgcgagga tcttttgagg aagcagcgta ccttcgacaa
     5101 cggatctatt ccccaccaga tccatctcgg cgagctgcac gctatcctcc gcaggcaaga
     5161 ggacttctat ccattcctta aggacaatcg cgagaagatt gaaaagatcc tcacttttag
     5221 aatcccttac tacgtcggac ccttggcccg tggcaacagc cgcttcgcct ggatgaccag
     5281 gaagtccgag gaaaccatca ccccatggaa cttcgaggag gtggttgaca agggggccag
     5341 cgctcagtcc ttcattgagc gcatgaccaa cttcgataag aacctgccca acgagaaagt
     5401 cctcccaaag cactcccttc tgtacgagta cttcactgtc tacaatgagt tgaccaaagt
     5461 gaagtacgtg accgagggta tgcgtaagcc tgccttcctc agcggcgagc agaagaaggc
     5521 catcgttgac ctcctgttca agaccaacag gaaagtcacc gtgaagcagc ttaaggagga
     5581 ctacttcaag aagatcgagt gctttgactc tgtcgagatc agcggagtgg aggatcgctt
     5641 caacgcttcc ttgggcactt accacgacct cctgaagatt atcaaggaca aggacttcct
     5701 cgacaacgag gagaacgaag atatcttgga ggacatcgtt cttaccctca ccctgttcga
     5761 ggacagagag atgattgagg aacgcctcaa gacctacgcc cacttgttcg acgataaagt
     5821 catgaagcaa cttaagcgta ggcgctacac cggatggggc aggctgtccc gtaagctcat
     5881 caacggtatc cgcgacaagc agagcggcaa gactattctc gacttcttga agtccgacgg
     5941 attcgccaat cgcaacttca tgcagctgat ccacgacgat tctcttacct tcaaggagga
     6001 catccaaaag gctcaagtgt ccggccaggg cgacagcctc cacgagcaca tcgccaacct
     6061 ggccgggagc cccgctatta agaagggaat cctccagacc gtcaaggtgg ttgacgagtt
     6121 ggtcaaggtg atgggcagac ataagccaga gaacatcgtc atcgagatgg ccagggaaaa
     6181 ccagaccacc cagaagggac agaagaactc ccgtgagcgc atgaagagga ttgaggaggg
     6241 catcaaggag cttggttccc agatcctcaa ggagcacccc gtggaaaaca cccaactcca
     6301 gaatgagaag ctgtatttgt actaccttca gaacgggcgc gatatgtacg ttgaccaaga
     6361 gctggacatc aaccgcctca gcgactacga cgtggatcac attgtccctc agtcctttct
     6421 caaggacgac tctatcgaca acaaggtgtt gactagaagc gataagaacc gcggcaagtc
     6481 cgacaacgtc ccatccgagg aggtggtcaa gaagatgaag aattactgga ggcagctgct
     6541 taacgccaag ctcatcaccc agcgcaagtt cgacaacttg accaaggccg agcgtggagg
     6601 cctcagcgaa ctggacaagg ctggattcat caagaggcaa cttgttgaaa cccgccagat
     6661 taccaagcac gtggcccaga tcctcgactc ccgtatgaac actaagtacg atgagaacga
     6721 caagctgatc cgcgaggtca aagtgattac cctcaagagc aagctcgtgt ctgacttcag
     6781 aaaggacttc caattctaca aggttaggga gatcaacaac taccaccacg cccacgatgc
     6841 ttaccttaat gccgtggtcg gcaccgcctt gatcaagaag taccccaagc tggagtccga
     6901 gttcgtgtat ggtgactaca aggtctacga cgttcgcaag atgatcgcta agagcgagca
     6961 ggagattggc aaggccaccg ccaagtactt cttctactcc aacatcatga acttctttaa
     7021 gaccgagatc actttggcta acggggagat ccgtaagcgc cctctcattg aaaccaacgg
     7081 agaaaccggc gagatcgtgt gggacaaggg cagggacttc gccaccgtca gaaaagtgct
     7141 gagcatgccc caagtcaaca tcgtgaagaa aaccgaggtt cagactggag gcttctccaa
     7201 ggagtctatc ctcccaaagc gcaattccga taagttgatt gcccgtaaga aggactggga
     7261 ccccaagaag tacggtggat tcgacagccc aaccgtcgcc tactccgtgc ttgtcgtggc
     7321 taaagttgag aagggcaaga gcaagaagct caagtccgtc aaggagctgc tcgggatcac
     7381 catcatggag cgcagctcct tcgagaagaa ccctattgat ttccttgagg ccaagggcta
     7441 caaggaagtg aagaaggact tgatcatcaa gctccccaag tactctctgt tcgagcttga
     7501 gaacggaagg aagcgtatgc tcgcctccgc tggcgagctg caaaagggaa acgagttggc
     7561 cctcccaagc aagtacgtca acttcctgta cctcgcctcc cactatgaga agctcaaggg
     7621 cagccccgag gacaacgaac agaagcagtt gtttgtggag cagcataagc actaccttga
     7681 cgagatcatt gagcagatca gcgagttctc caagcgcgtt atcctggctg acgccaatct
     7741 cgataaagtc ctttctgcct acaacaagca cagggacaag cctatcagag agcaggctga
     7801 aaacattatc cacttgttca ccctcaccaa cctgggtgcc ccagccgcct tcaagtactt
     7861 cgacactacc atcgaccgca agcgttacac ctccaccaag gaagtgctcg atgctaccct
     7921 tatccaccag agcattactg ggttgtacga aaccaggatc gacctgtccc aactcggcgg
     7981 agacaagcgc cccgccgcca ccaagaaggc tggccaggcc aagaagaaga agtaacccgg
     8041 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     8101 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     8161 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     8221 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     8281 atgttactag atcgctcgaa ttcgtacagc attacgtagg tacgttttct ttttcttccc
     8341 ggagagatga tacgataatc atgtaaaccc agaatttaaa aaatattctt tactataaaa
     8401 attttaatta gggaacgtat tattttttac atgacacctt ttgagaaaga gggacttgta
     8461 atatgggaca aatgaacaat ttctaagaaa tgggcatatg actctcagta caatggacca
     8521 aattccctcc agtcggccca gcaatacaaa gggaaagaaa tgagggggcc cacaggccac
     8581 ggcccacttt tctccgtggt ggggagatcc agctagaggt ccggcccaca agtggccctt
     8641 gccccgtggg acggtgggat tgcagagcgc gtgggcggaa acaacagttt agtaccacct
     8701 cgctcacgca acgacgcgac cacttgctta taagctgctg cgctgaggct cagcttgaga
     8761 cgttcgaagc ttctgtcgtc tcagttttag agctagaaat agcaagttaa aataaggcta
     8821 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgtcg acaggcctta
     8881 agggccagat cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac
     8941 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     9001 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     9061 gccaaccaca gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc
     9121 aaccacccaa acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct
     9181 cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc
     9241 ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga
     9301 tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca
     9361 caggatgatc tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact
     9421 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt
     9481 cgccgccgac gccgttgatg tggacgccga aaaggatcta ggtgaagatc ctttttgata
     9541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgatta ccgcctttga
    10321 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10381 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
    10441 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtaaccgtg
    10501 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg gccggcgagc
    10561 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
    10621 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
    10681 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
    10741 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
    10801 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
    10861 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
    10921 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
    10981 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
    11041 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
    11101 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
    11161 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
    11221 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
    11281 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
    11341 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
    11401 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
    11461 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
    11521 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
    11581 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
    11641 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
    11701 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
    11761 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
    11821 ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
    11881 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
    11941 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
    12001 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
    12061 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
    12121 ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac tgatggcggt
    12181 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
    12241 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
    12301 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
    12361 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
    12421 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
    12481 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
    12541 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
    12601 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    12661 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    12721 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    12781 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    12841 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
    12901 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    12961 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    13021 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    13081 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    13141 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    13201 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    13261 ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga ccgaaacctt
    13321 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
    13381 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
    13441 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
    13501 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
    13561 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
    13621 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
    13681 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
    13741 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
    13801 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
    13861 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
    13921 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
    13981 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
    14041 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
    14101 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
    14161 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
    14221 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
    14281 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
    14341 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
    14401 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
    14461 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct
    14521 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
    14581 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
    14641 tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca acatagcttg
    14701 cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac tttgcgatcg
    14761 tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc tctcgcaacg
    14821 ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc cgtcgctcac
    14881 cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg cctaaccggc
    14941 tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc agcggc
//
