LOCUS       C143p6ioRAct-UZm       16007 bp    DNA     circular SYN 20-OCT-2016
DEFINITION  Ligation of C164p7ioR-UZmCwtoi-Bbs* into C141p6ioRAct-LH*
ACCESSION   C143p6ioRAct-UZm
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 16007)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  C141p6ioRAct-LH*
               Fragment ends: SfiI
               Fragment size: 9416
            Insert molecule:  C164p7ioR-UZmCwtoi-Bbs*
               Fragment ends: SfiI
               Fragment size: 6591
            Sequencing primers:
            SphR	GGGTGGTTGAACAGAAGTCAT
            SBx	GGTCCTTTTGTTCATTCTCAA
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    313..1710
                     /gene="pAct"
                     /product="Actin Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1140..1268
                     /gene="del"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1733..1737
                     /gene="Kozak"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1738..2307
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    2308..2496
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             2497..2952
                     /gene="'hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2970..3260
                     /gene="'E9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3315..4823
                     /gene="Ubi-int"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4302..4821
                     /gene="Intron"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4854..4874
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             4890..9044
                     /gene="'Cas9WT"
                     /translation="PDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAG
                     QAKKKK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="'Cas9WToi"
                     /SECDescr="no intronsequence"
     misc_feature    8994..9041
                     /gene="NLS"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9050..9303
                     /gene="NOS-T"
                     /product="Nos-Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="NOS-T"
                     /SECDescr="Nos-Terminator"
     misc_feature    9313..9765
                     /gene="U6Os'"
                     /product="U6Os C4"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9766..9794
                     /gene="insert target RNA"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             9795..9824
                     /product="hairpin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    9795..9877
                     /product="gRNA scaffold"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    9963..9986
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    10521..11218
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    11506..14293
                     /gene="'pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             14477..15487
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata
      361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt
      421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa
      481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt
      541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt
      601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac
      661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta
      721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac
      781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc
      841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac
      901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt
      961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt
     1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc
     1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc
     1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc
     1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc
     1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta
     1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga
     1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct
     1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc
     1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat
     1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta
     1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat
     1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg
     1741 aagaaaccgg agctgaccgc cacttccgtc gaaaagttcc tcatcgagaa gtttgatagc
     1801 gtgtctgacc ttatgcagtt gtcggagggc gaagagtccc gcgcgttcag ctttgatgtt
     1861 ggcgggcgcg ggtacgtcct ccgcgtgaac tcttgcgctg acggcttcta taaagatcgg
     1921 tacgtttaca ggcatttcgc ctcggcggcc ctgcctattc ccgaagtcct cgacatcggc
     1981 gagttttccg agagccttac gtattgtatt tcccgccggg ctcaaggggt gaccctccag
     2041 gatctgccgg aaactgagtt gcctgcggtt ctccagcccg tcgccgaggc tatggacgcc
     2101 atcgccgcgg cggatctttc tcaaacgtcg ggcttcgggc cgttcggccc tcaggggatt
     2161 ggccagtaca ccacttggag ggactttatc tgcgctatcg ccgatccgca cgtgtatcat
     2221 tggcaaaccg ttatggacga tacggtctcc gcgagcgtgg cccaggctct ggacgaactc
     2281 atgttgtggg ccgaggattg ccccgaggta agtttctgct tctacctttg atatatatat
     2341 aataattatc attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga
     2401 atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac
     2461 ttttctaata tatgaccaaa atttgttgat gtgcaggtcc gccacctggt tcatgcggac
     2521 ttcggctcta ataacgtgct caccgataat gggcggatta ctgccgtcat cgactggtcc
     2581 gaagctatgt ttggcgattc gcagtacgaa gtggcgaaca ttttcttctg gaggccttgg
     2641 cttgcctgca tggaacagca aacgcgctac tttgagcgga ggcacccgga gctggcgggg
     2701 tctccccgcc tcagggccta tatgttgcgg atcggcctcg accagcttta ccagtccctg
     2761 gttgatggga atttcgacga tgctgcctgg gcgcaaggcc gctgtgacgc tattgtcagg
     2821 agcggcgcgg ggaccgtggg ccgcactcag atcgcccggc ggtcggctgc cgtttggacg
     2881 gatgggtgcg tcgaagtgct cgcggacagc ggcaacaggc ggccttccac ccgcccgcgg
     2941 gccaaggagt gatccggaat cgcgttcgag tattatggca ttgggaaaac tgtttttctt
     3001 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg
     3061 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa
     3121 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat
     3181 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt
     3241 aatatgagga gtaaaacact gaagcctgca ggcatgcaag ctgatccact agaggccatg
     3301 gcggccgcac tagtctgcag tgcagcgtga cccggtcgtg cccctctcta gagataatga
     3361 gcattgcatg tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa
     3421 gtgcagttta tctatcttta tacatatatt taaactttac tctacgaata atataatcta
     3481 tagtactaca ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc
     3541 taaaggacaa ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat
     3601 gtgttctcct ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag
     3661 tacatccatt tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta
     3721 ttttattcta ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt
     3781 taataattta gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt
     3841 taagaaatta aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg
     3901 ttaaacgccg tcgatcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt
     3961 cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga
     4021 gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg
     4081 gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg
     4141 ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac
     4201 accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc
     4261 agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc
     4321 cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag
     4381 ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg
     4441 ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt
     4501 ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga tctaggatag
     4561 gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat
     4621 tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat
     4681 tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc
     4741 cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt
     4801 tgtttggtgt tacttctgca ggtactagat cctccaccga aatggcctcc ggacccaaga
     4861 agaagcgcaa agtgggaatc cacggagctc cagacaagaa gtacagcatt ggtctggaca
     4921 tcgggaccaa ctccgtcggc tgggccgtta tcaccgatga gtacaaagtg ccttctaaga
     4981 agttcaaagt cctcggaaac actgacaggc acagcatcaa gaagaacttg attggcgccc
     5041 ttctcttcga ctccggcgaa accgctgagg ccacccgtct gaagcgcacc gccagaaggc
     5101 gctacacccg tcgcaagaat aggatctgct acctccagga gatcttcagc aacgagatgg
     5161 ccaaagtgga cgattccttc tttcaccgtt tggaggagag cttccttgtc gaggaggaca
     5221 agaagcacga gcgccatccc atcttcggaa acattgttga cgaggtggct taccacgaga
     5281 agtatccaac tatctaccac ctcagaaaga agctggtgga ctccaccgac aaggccgatt
     5341 tgaggctcat ctaccttgcc ctcgctcaca tgatcaagtt ccgcggtcac ttcctgattg
     5401 agggcgacct caaccccgac aactctgacg tcgataagtt gttcatccaa ctggtccaga
     5461 cctacaacca gcttttcgag gagaatccta tcaacgcctc cggggttgac gccaaggcta
     5521 ttctcagcgc ccgtctgtcc aagagccgca ggcttgagaa cttgatcgcc cagctcccag
     5581 gagagaagaa gaacggcctc ttcggaaacc tgatcgctct ctccttgggc cttaccccta
     5641 acttcaagtc taattttgac ctcgccgagg acgccaagct gcaactcagc aaggacacct
     5701 acgatgacga cttggacaac ctgctcgccc agatcggcga tcagtacgct gaccttttct
     5761 tggccgccaa gaacctctcc gacgctattc tgctctccga catccttcgc gtgaacactg
     5821 agatcaccaa ggccccactg agcgcctcca tgatcaagcg ttacgacgaa caccaccaag
     5881 atttgaccct cctcaaggct cttgtcagac agcagctgcc cgagaagtac aaggagattt
     5941 tcttcgacca gagcaagaac ggatacgccg gttacatcga cggcggggcc tcccaagagg
     6001 agttctacaa gttcatcaag cccatcttgg agaagatgga cggcaccgaa gagctgctcg
     6061 tgaagctcaa ccgcgaggat cttttgagga agcagcgtac cttcgacaac ggatctattc
     6121 cccaccagat ccatctcggc gagctgcacg ctatcctccg caggcaagag gacttctatc
     6181 cattccttaa ggacaatcgc gagaagattg aaaagatcct cacttttaga atcccttact
     6241 acgtcggacc cttggcccgt ggcaacagcc gcttcgcctg gatgaccagg aagtccgagg
     6301 aaaccatcac cccatggaac ttcgaggagg tggttgacaa gggggccagc gctcagtcct
     6361 tcattgagcg catgaccaac ttcgataaga acctgcccaa cgagaaagtc ctcccaaagc
     6421 actcccttct gtacgagtac ttcactgtct acaatgagtt gaccaaagtg aagtacgtga
     6481 ccgagggtat gcgtaagcct gccttcctca gcggcgagca gaagaaggcc atcgttgacc
     6541 tcctgttcaa gaccaacagg aaagtcaccg tgaagcagct taaggaggac tacttcaaga
     6601 agatcgagtg ctttgactct gtcgagatca gcggagtgga ggatcgcttc aacgcttcct
     6661 tgggcactta ccacgacctc ctgaagatta tcaaggacaa ggacttcctc gacaacgagg
     6721 agaacgaaga tatcttggag gacatcgttc ttaccctcac cctgttcgag gacagagaga
     6781 tgattgagga acgcctcaag acctacgccc acttgttcga cgataaagtc atgaagcaac
     6841 ttaagcgtag gcgctacacc ggatggggca ggctgtcccg taagctcatc aacggtatcc
     6901 gcgacaagca gagcggcaag actattctcg acttcttgaa gtccgacgga ttcgccaatc
     6961 gcaacttcat gcagctgatc cacgacgatt ctcttacctt caaggaggac atccaaaagg
     7021 ctcaagtgtc cggccagggc gacagcctcc acgagcacat cgccaacctg gccgggagcc
     7081 ccgctattaa gaagggaatc ctccagaccg tcaaggtggt tgacgagttg gtcaaggtga
     7141 tgggcagaca taagccagag aacatcgtca tcgagatggc cagggaaaac cagaccaccc
     7201 agaagggaca gaagaactcc cgtgagcgca tgaagaggat tgaggagggc atcaaggagc
     7261 ttggttccca gatcctcaag gagcaccccg tggaaaacac ccaactccag aatgagaagc
     7321 tgtatttgta ctaccttcag aacgggcgcg atatgtacgt tgaccaagag ctggacatca
     7381 accgcctcag cgactacgac gtggatcaca ttgtccctca gtcctttctc aaggacgact
     7441 ctatcgacaa caaggtgttg actagaagcg ataagaaccg cggcaagtcc gacaacgtcc
     7501 catccgagga ggtggtcaag aagatgaaga attactggag gcagctgctt aacgccaagc
     7561 tcatcaccca gcgcaagttc gacaacttga ccaaggccga gcgtggaggc ctcagcgaac
     7621 tggacaaggc tggattcatc aagaggcaac ttgttgaaac ccgccagatt accaagcacg
     7681 tggcccagat cctcgactcc cgtatgaaca ctaagtacga tgagaacgac aagctgatcc
     7741 gcgaggtcaa agtgattacc ctcaagagca agctcgtgtc tgacttcaga aaggacttcc
     7801 aattctacaa ggttagggag atcaacaact accaccacgc ccacgatgct taccttaatg
     7861 ccgtggtcgg caccgccttg atcaagaagt accccaagct ggagtccgag ttcgtgtatg
     7921 gtgactacaa ggtctacgac gttcgcaaga tgatcgctaa gagcgagcag gagattggca
     7981 aggccaccgc caagtacttc ttctactcca acatcatgaa cttctttaag accgagatca
     8041 ctttggctaa cggggagatc cgtaagcgcc ctctcattga aaccaacgga gaaaccggcg
     8101 agatcgtgtg ggacaagggc agggacttcg ccaccgtcag aaaagtgctg agcatgcccc
     8161 aagtcaacat cgtgaagaaa accgaggttc agactggagg cttctccaag gagtctatcc
     8221 tcccaaagcg caattccgat aagttgattg cccgtaagaa ggactgggac cccaagaagt
     8281 acggtggatt cgacagccca accgtcgcct actccgtgct tgtcgtggct aaagttgaga
     8341 agggcaagag caagaagctc aagtccgtca aggagctgct cgggatcacc atcatggagc
     8401 gcagctcctt cgagaagaac cctattgatt tccttgaggc caagggctac aaggaagtga
     8461 agaaggactt gatcatcaag ctccccaagt actctctgtt cgagcttgag aacggaagga
     8521 agcgtatgct cgcctccgct ggcgagctgc aaaagggaaa cgagttggcc ctcccaagca
     8581 agtacgtcaa cttcctgtac ctcgcctccc actatgagaa gctcaagggc agccccgagg
     8641 acaacgaaca gaagcagttg tttgtggagc agcataagca ctaccttgac gagatcattg
     8701 agcagatcag cgagttctcc aagcgcgtta tcctggctga cgccaatctc gataaagtcc
     8761 tttctgccta caacaagcac agggacaagc ctatcagaga gcaggctgaa aacattatcc
     8821 acttgttcac cctcaccaac ctgggtgccc cagccgcctt caagtacttc gacactacca
     8881 tcgaccgcaa gcgttacacc tccaccaagg aagtgctcga tgctaccctt atccaccaga
     8941 gcattactgg gttgtacgaa accaggatcg acctgtccca actcggcgga gacaagcgcc
     9001 ccgccgccac caagaaggct ggccaggcca agaagaagaa gtaacccggg atcgttcaaa
     9061 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     9121 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     9181 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     9241 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     9301 tcgctcgaat tcgtacagca ttacgtaggt acgttttctt tttcttcccg gagagatgat
     9361 acgataatca tgtaaaccca gaatttaaaa aatattcttt actataaaaa ttttaattag
     9421 ggaacgtatt attttttaca tgacaccttt tgagaaagag ggacttgtaa tatgggacaa
     9481 atgaacaatt tctaagaaat gggcatatga ctctcagtac aatggaccaa attccctcca
     9541 gtcggcccag caatacaaag ggaaagaaat gagggggccc acaggccacg gcccactttt
     9601 ctccgtggtg gggagatcca gctagaggtc cggcccacaa gtggcccttg ccccgtggga
     9661 cggtgggatt gcagagcgcg tgggcggaaa caacagttta gtaccacctc gctcacgcaa
     9721 cgacgcgacc acttgcttat aagctgctgc gctgaggctc agcttggtct tcttcgaagc
     9781 ttctgtgaag accagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     9841 caacttgaaa aagtggcacc gagtcggtgc tttttttgtc gacaggcctt aagggccaga
     9901 tcttgggccc ggtacccgat cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt
     9961 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
    10021 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
    10081 agggttcccc tcgggagtgc ttggcattcc gtgcgataat gacttctgtt caaccaccca
    10141 aacgtcggaa agcctgacga cggagcagca ttccaaaaag atcccttggc tcgtctgggt
    10201 cggctagaag gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc
    10261 gcagcgccga aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt
    10321 cgctctttcg gccgacgtcc tggccagtca tcacgcgcca aagttccgtc acaggatgat
    10381 ctggcgcgag ttgctggatc tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa
    10441 tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga
    10501 cgccgttgat gtggacgccg aaaaggatct aggtgaagat cctttttgat aatctcatga
    10561 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10621 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10681 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10741 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10801 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10861 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10921 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10981 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11041 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11101 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11161 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11221 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11281 tctttcctgc gttatcccct gattctgtgg ataaccgatt accgcctttg agtgagctga
    11341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    11461 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtaaccgt gcggctgcat
    11521 gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag cttggccgct
    11581 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
    11641 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
    11701 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
    11761 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
    11821 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
    11881 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
    11941 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
    12001 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
    12061 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
    12121 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
    12181 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
    12241 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
    12301 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
    12361 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
    12421 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
    12481 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
    12541 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
    12601 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
    12661 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
    12721 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
    12781 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggcggcgca
    12841 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
    12901 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
    12961 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
    13021 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
    13081 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
    13141 gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
    13201 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
    13261 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
    13321 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
    13381 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
    13441 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
    13501 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
    13561 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
    13621 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
    13681 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
    13741 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
    13801 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
    13861 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
    13921 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    13981 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    14041 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    14101 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    14161 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    14221 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    14281 acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct tgcgctcgtt
    14341 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
    14401 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
    14461 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
    14521 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
    14581 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
    14641 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
    14701 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
    14761 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
    14821 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
    14881 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
    14941 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
    15001 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
    15061 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
    15121 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
    15181 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
    15241 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
    15301 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
    15361 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
    15421 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
    15481 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
    15541 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
    15601 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct
    15661 ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt gcttggtcgt
    15721 tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg
    15781 cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac gccatccgac
    15841 ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg
    15901 gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg
    15961 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggc
//
