	   SnapGene      ÊCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCTCTAGAGGCCATGGCGGCCGCACTAGATCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAGAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCGAAAGGACAGTAGAAAAGGAAGATGGCTTCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTACCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAACATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACTAGTTGGATCCCCCGGGCTGCAGGAATTCAAGCTTACGCGTTGTACAGGAGTACCGCCCCGTCCGGTCCTGCCCGTCACCGAGATCAGCGGAGAGTTCATGGGCAAGGGCGAGGAACTGTTCACTGGCGTGGTCCCAATCCTGGTGGAACTGGATGGTGATGTGAACGGGCACAAGTTCTCCGTCAGCGGAGAGGGTGAAGGTGATGCCACCTACGGAAAGCTCACCCTGAAGTTCATCTGCACTACCGGAAAGCTCCCTGTTCCGTGGCCAACCCTCGTCACCACTTTCACCTACGGTGTTCAGTGCTTCTCCCGTTACCCAGATCACATGAAGCAGCATGACTTCTTCAAGAGCGCCATGCCCGAAGGCTACGTGCAAGAAAGGACTATCTTCTTCAAGGATGACGGGAACTACAAGACACGTGCCGAAGTCAAGTTCGAAGGTGATACCCTGGTGAACCGCATCGAGCTGAAAGGTAAGTTTCTGCTTCTACCTTTGATATATATATAATAATTATCATTAATTAGTAGTAATATAATATTTCAAATATTTTTTTCAAAATAAAAGAATGTAGTATATAGCAATTGCTTTTCTGTAGTTTATAAGTGTGTATATTTTAATTTATAACTTTTCTAATATATGACCAAAATTTGTTGATGTGCAGGTATCGATTTCAAGGAAGATGGAAACATCCTCGGACACAAGCTGGAGTACAACTACAACTCCCACAACGTATACATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCAGGCACAACATCGAAGATGGAAGCGTGCAACTGGCGGACCACTACCAGCAGAACACGCCCATCGGCGATGGCCCTGTCCTGCTGCCGGACAACCATTACCTGTCCACGCAATCTAAGCTCTCCAAGGACCCCAACGAGAAGAGGGACCACATGGTCCTGCTGGAGTTCGTGACGGCTGCTGGGATCACGCATGGCATGGATGAACTCTACAAGTGACCCGGTCGACCCTGCTTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATCCTTACCGCCGGTTTCGGTTCATTCTAATGAATATATCACCCGTTACTATCGTATTTTTATGAATAATATTCTCCGTTCAATTTACTGATTGTCCTCGACAGGCCTTAAGGGCCAGATCTTGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTT  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="10"><Feature recentID="0" name="35S-Pro" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="106-528" color="#2f7a3e" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;35S-Pro&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Promotor-Region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="TATA" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="494-500" color="#7b5ea7" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;TATA&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;TATA-box&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="translation"><V text="YI"/></Q></Feature><Feature recentID="2" name="mEGFP" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="627-1005" color="#d9a400" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mEGFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;mEGFP&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="F64L/S65T - enhanced fluorescence" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="816-821" color="#7b5ea7" type="standard"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;F64L/S65T - enhanced fluorescence&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ST-LS1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1006-1194" color="#d9a400" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;ST-LS1&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Intron&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="mEGFP" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1195-1532" color="#d9a400" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mEGFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;mEGFP&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="A206K - monomeric protein" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1431-1433" color="#7b5ea7" type="standard"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;A206K - monomeric protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="OCS" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1542-1737" color="#b8322f" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;OCS&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Terminator&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="Amp" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2553-3413" color="#3557b7" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;Amp&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;ORF-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="ColE1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3456-4172" color="#8b8f86" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;ColE1&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;ColE1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>   Ø<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/></Primers>  :<Notes>
<UUID>0a84ad87-04a3-5ff0-9696-dfb80d546695</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>p35s-LmEGFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html&gt;&lt;body&gt;p35s-LmEGFP (D360): Ligation of GFP-Hind-Sal* into B629p35s-LGFP*&lt;/body&gt;&lt;/html&gt;</Description>
<Created UTC="12:0:0">2020.8.26</Created>
<LastModified UTC="12:0:0">2020.8.26</LastModified>
<CreatedBy>DNA Cloning Service</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>&lt;html&gt;&lt;body&gt;p35s-LmEGFP from DNA Cloning Service, Hamburg: &lt;a href=&quot;https://dna-cloning.com/vectors/puc/p35s-LmEGFP&quot;&gt;https://dna-cloning.com/vectors/puc/p35s-LmEGFP&lt;/a&gt;&lt;br&gt;
&lt;br&gt;
Vector molecule:&amp;nbsp;&amp;nbsp;B629p35s-LGFP*&lt;br&gt;
&amp;nbsp;&amp;nbsp;&amp;nbsp;Fragment ends: SalI and HindIII&lt;br&gt;
&amp;nbsp;&amp;nbsp;&amp;nbsp;Fragment size: 3574&lt;br&gt;
Insert molecule:&amp;nbsp;&amp;nbsp;GFP-Hind-Sal*&lt;br&gt;
&amp;nbsp;&amp;nbsp;&amp;nbsp;Fragment ends: HindIII and SalI&lt;br&gt;
&amp;nbsp;&amp;nbsp;&amp;nbsp;Fragment size: 979&lt;br&gt;
Sequencing primers:&lt;br&gt;
YFPDCR CCATCTTCCTTGAAATCGATAC&lt;br&gt;
LH1 CCCAAGATCTGGCCCTT&lt;/body&gt;&lt;/html&gt;</Comments>
</Notes>
