LOCUS C834p35s-Q4GFP 6593 bp DNA circular SYN 27-FEB-2019 DEFINITION Ligation of m4GFP_BamEco* into p35-OCS-BM* ACCESSION C834p35s-Q4GFP KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct FEATURES Location/Qualifiers misc_feature 106..528 /label="35S-Pro" /SECDrawAs="Region" CDS 494..500 /label="TATA" /SECDrawAs="Gene" CDS 535..1313 /label="'Q1'" /SECDrawAs="Gene" CDS 549..3559 /label="ORF-1" /SECDrawAs="Gene" misc_feature 1263..1313 /label="Linker" /SECDrawAs="Region" CDS 1263..2078 /label="Q2'" /SECDrawAs="Gene" misc_feature 2028..2078 /label="Linker" /SECDrawAs="Region" CDS 2048..2822 /label="Q3" /SECDrawAs="Gene" misc_feature 2793..2843 /label="Linker" /SECDrawAs="Region" CDS 2793..3559 /label="Q4''" /SECDrawAs="Gene" misc_feature 3582..3777 /label="OCS" /SECDrawAs="Region" misc_feature 4593..5453 /label="Amp" /SECDrawAs="Region" misc_feature 5496..6212 /label="ColE1" /SECDrawAs="Region" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc 121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatcct 541 ccaaaacaat gggcaaggga gaggagctct ttaccggcgt ggtgcccatt ctggtcgaac 601 ttgacggaga cgtcaacggc cataaattta gtgtctctgg agaaggagag ggagatgcca 661 cttacggtaa gcttaccctc aagttcatct gcacgacggg taaattgcca gtgccttggc 721 cgacattggt cacgaccttc acctacggtg ttcagtgctt ttctcgctac ccggaccaca 781 tgaagcagca tgacttcttc aagtccgcta tgccagaggg atacgtgcag gagcgcacta 841 tcttcttcaa ggatgacggc aactacaaga ccagggccga agtcaagttc gagggggaca 901 ccttggtgaa tcgcatcgag cttaagggca ttgacttcaa ggaagacgga aacattctcg 961 gccataagct ggagtacaat tacaactcgc acaacgtcta cattatggcc gataagcaga 1021 agaacgggat caaggtgaat ttcaagatcc gtcacaacat tgaggatggc agcgttcagc 1081 tcgcagacca ctatcaacag aacactccca tcggagacgg ccctgtcttg cttccggaca 1141 accactacct gtccacccag tcaaagctca gcaaggaccc aaacgaaaag agggaccaca 1201 tggtgctact cgagttcgta accgctgccg ggatcaccca cggaatggat gaattgtaca 1261 agggggtccc gcccagaccc gttttgcccg ttacagagat ctccggagag ttcatgggta 1321 agggcgagga gctgtttacc ggggtcgtgc cgattctcgt cgagctggat ggagatgtga 1381 acggccacaa gttcagtgtt tccggagagg gcgagggaga tgctacgtac ggcaaactca 1441 ccctgaagtt catctgcacc actggtaagc tccccgtgcc atggcccacc cttgtcacca 1501 ccttcacata cggcgtgcaa tgcttcagcc gttaccctga ccatatgaag cagcacgatt 1561 tctttaagtc tgccatgccg gaggggtacg tgcaggagag gaccatcttc tttaaggacg 1621 acgggaacta caagactcga gctgaggtga agttcgaggg cgacaccctg gtcaaccgta 1681 tcgagctcaa gggaattgac ttcaaggagg atggcaacat cttgggtcac aagctggagt 1741 acaactataa ctcccacaac gtctacatca tggctgacaa gcagaagaat ggcatcaagg 1801 tgaacttcaa gatcaggcac aacatcgagg atggatcggt ccaacttgcc gaccactatc 1861 aacaaaatac tcccattggc gacgggccag tgctgctccc tgacaatcac tacttgagca 1921 cccaatccaa gctgtcaaag gaccccaatg agaagcgcga ccacatggtc ctccttgagt 1981 tcgttaccgc cgccggcatc acccatggta tggacgagtt gtacaaggga gtgccaccga 2041 gacccgtcct cccagtgact gaaatttccg gcgagttcat gggaaagggc gaggagctct 2101 tcaccggggt tgtccccatt cttgtggagc tcgacggcga cgtcaacgga cacaagttca 2161 gcgtgtccgg cgagggtgaa ggcgatgcaa cctacggaaa gctgacactt aagttcatct 2221 gcactaccgg caaactccct gttccgtggc ccacgcttgt caccactttc acctacgggg 2281 tgcagtgctt tagccggtac cctgaccaca tgaagcaaca cgatttcttc aagtctgcca 2341 tgcctgaggg atacgtccag gagcgcacca ttttcttcaa ggacgatggt aactacaaga 2401 cccgtgctga agtgaagttc gagggtgaca ctctcgtaaa caggattgag ctgaagggca 2461 tcgacttcaa ggaggacgga aacatcttgg gacacaagct cgagtacaac tacaattccc 2521 acaacgttta catcatggct gataagcaaa agaacggaat caaggttaac ttcaagatcc 2581 gccacaacat cgaggacggc tcggttcagc tggccgacca ctaccagcaa aatacaccca 2641 tcggggacgg cccagtgctc cttcccgaca atcactacct gagcacccag tccaagctct 2701 ccaaggaccc gaacgagaag cgtgaccaca tggtcctgct agagttcgtg accgccgcgg 2761 gaattaccca cggtatggac gaactctaca agggcgtccc tccaaggccc gttcttcccg 2821 tgacagagat ctccggcgag tttatgggaa agggggagga actgttcacc ggcgtcgtgc 2881 caatcttggt cgaactcgac ggagacgtta acggtcacaa gttcagcgtc tcaggtgagg 2941 gtgagggaga tgccacctac ggcaagctca ctctgaagtt catttgcacc accggaaaat 3001 tgcctgttcc ttggccaacc cttgtgacta ccttcaccta cggagtccag tgcttctcca 3061 gatacccaga tcacatgaag cagcacgact tcttcaagag cgctatgccc gagggctacg 3121 tgcaggagcg taccatcttc ttcaaggacg acggaaacta caagacccgc gccgaagtta 3181 agttcgaggg agacacgctc gtcaaccgca tcgagctgaa gggaatcgat ttcaaggagg 3241 acggcaacat cctcggccac aagcttgagt acaactacaa cagccacaac gtgtacatta 3301 tggccgacaa gcagaagaac ggcatcaagg tcaacttcaa gatccgtcat aacatcgagg 3361 atgggtccgt gcagcttgca gaccactatc agcaaaatac cccgattggc gacggtcctg 3421 ttctcctgcc agacaaccac tacctctcga cccagagcaa gctttccaaa gatcccaacg 3481 aaaagcgcga tcacatggtc ttgctggagt tcgtgactgc tgccggaatc acccacggca 3541 tggacgagct ctacaagtga attcaagctt acgcgtgtcg accctgcttt aatgagatat 3601 gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa 3661 cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc 3721 acccgttact atcgtatttt tatgaataat attctccgtt caatttactg attgtcctcg 3781 acaggcctta agggccagat cttgggcccg gtacccaatt cgccctatag tgagtcgtat 3841 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 3901 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 3961 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta 4021 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 4081 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 4141 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 4201 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 4261 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 4321 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 4381 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 4441 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4561 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 4621 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4681 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4741 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4801 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4861 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 4921 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 4981 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 5041 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 5101 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 5161 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 5221 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 5281 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 5341 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 5401 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 5461 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5761 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6001 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 6361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 6421 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 6481 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 6541 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa ttt //