LOCUS       pHC-5                   1709 bp    DNA     circular SYN 20-SEP-2021
DEFINITION  pHC-5 (E728): pHC 5 Circularized
ACCESSION   pHC-5
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     DNA Cloning Service, https://dna-cloning.com/vectors/puc/pHC-5
FEATURES             Location/Qualifiers
     CDS             30..50
                     /label="O1"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
     CDS             107..127
                     /label="O2"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
     CDS             182..1042
                     /label="ORF-1"
                     /note="color: #D9A400"
                     /ApEinfo_fwdcolor="#D9A400"
                     /ApEinfo_revcolor="#D9A400"
     misc_feature    1045..1709
                     /label="ColE1"
                     /note="color: #8B8F86"
                     /ApEinfo_fwdcolor="#8B8F86"
                     /ApEinfo_revcolor="#8B8F86"
     misc_feature    1267
                     /label="LC"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
ORIGIN
        1 aacgccagca acgcggcctt tatacagata attgtgagcg gataacaatt aataggcgta
       61 tcacgaggct cgagtgtagg ccttggatcc ggccaggaac cgtaaaaaat gtgagcgagt
      121 aacaacctga tcttgagagc tattctagtc actcataaga tatctgcata ggaggtcagc
      181 catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      301 ccgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      421 ccgtgttgac gccgggcaag agcaactcgg acaccgcata cactattctc agaatgactt
      481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      721 gccagcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc
      901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      961 cacgacgggg agccaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1021 ctcactgatt aagcattggt gatctcatgg ccaaaatccc ttaacgtgag ttttcgttcc
     1081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1141 gcgtaatctg ctgcttgcaa acaaaaaaac catcgctacc agcggtggtt tgtttgccgg
     1201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1261 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1441 cggggggttc gtgctcacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1681 gctcgtcagg ggggcggagc ctatggaaa
//