LOCUS       pHC2-T7                 2479 bp    DNA     circular SYN 27-JAN-2017
DEFINITION  pHC2-T7 (C301): Ligation of Gus-Kpn-BsmB* into HCT7-Bsa-Kpn*
ACCESSION   pHC2-T7
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     DNA Cloning Service, https://dna-cloning.com/vectors/puc/pHC2-T7
FEATURES             Location/Qualifiers
     misc_feature    complement(114..154)
                     /label="T7T"
                     /note="color: #A6ADA2"
                     /ApEinfo_fwdcolor="#A6ADA2"
                     /ApEinfo_revcolor="#A6ADA2"
     misc_feature    155..177
                     /label="T7P"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
     CDS             178..863
                     /label="'Gus"
                     /note="color: #D9A400"
                     /ApEinfo_fwdcolor="#D9A400"
                     /ApEinfo_revcolor="#D9A400"
     misc_feature    complement(866..888)
                     /label="T7P"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
     misc_feature    889..929
                     /label="T7T"
                     /note="color: #A6ADA2"
                     /ApEinfo_fwdcolor="#A6ADA2"
                     /ApEinfo_revcolor="#A6ADA2"
     CDS             1009..1869
                     /label="ORF-1"
                     /note="color: #D9A400"
                     /ApEinfo_fwdcolor="#D9A400"
                     /ApEinfo_revcolor="#D9A400"
     misc_feature    join(1872..2479,1..57)
                     /label="ColE1"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
     misc_feature    2094
                     /label="LC"
                     /note="color: #7B5EA7"
                     /ApEinfo_fwdcolor="#7B5EA7"
                     /ApEinfo_revcolor="#7B5EA7"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcctttat acagataata ggcgtatcac gaggccgaat tcccaaaaaa
      121 cccctcaaga cccgtttaga ggccccaagg ggtttaatac gactcactat agggagacct
      181 gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat
      241 cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca
      301 attgctgtgc caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg
      361 ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc
      421 gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg
      481 atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc
      541 gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg
      601 ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc
      661 tttaactatg ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg
      721 gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg
      781 caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt
      841 gcaactggac aaggcactag cggtctctcc ctatagtgag tcgtattaaa ccccttgggg
      901 cctctaaacg ggtcttgagg ggttttttgc cggtaccagg ccaggaaccg taaatgatct
      961 tgagagctat tctagtcact cataagatat ctgcatagga ggtcagccat gagtattcaa
     1021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcccg agtgggttac
     1141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     1261 gggcaagagc aactcggtca ccgcatacac tattctcaga atgacttggt tgagtactca
     1321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc agcagcaatg
     1561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1681 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt
     1741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1861 cattggtgat ctcatggcca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2281 ctcacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2461 tcctgtcggg tttcgccac
//