	   SnapGene      #èAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCTCTCACCATCACCACCATCACTCCGGCCTGGTGCCGCGCGGCAGCCATATGAACCCGAAGAAGAAACGTAAAGTTAACGGTACCAACAACTTCCAGAACTTCATCGGCATCTCTTCCCTTCAGAAAACTCTGCGCAACGCTCTGATCCCGACCGAAACTACCCAGCAGTTTATTGTTAAGAACGGTATCATCAAAGAGGACGAACTGCGTGGCGAAAACCGTCAAATCCTGAAAGATATCATGGACGACTACTACCGTGGTTTCATCAGCGAAACTCTCTCTTCCATTGATGACATCGACTGGACCTCTCTGTTCGAAAAGATGGAAATCCAGCTGAAGAACGGCGACAACAAAGATACTCTGATCAAAGAACAGACAGAATATCGCAAAGCAATCCACAAGAAATTCGCGAATGACGACCGTTTTAAGAACATGTTCTCAGCTAAACTGATCTCCGATATTCTGCCGGAATTCGTAATCCACAACAACAACTACTCTGCAAGCGAAAAGGAAGAGAAAACCCAAGTGATCAAACTGTTCTCTCGTTTTGCTACTTCCTTCAAAGACTACTTCAAGAACCGTGCCAACTGCTTCTCTGCTGACGATATCAGTTCCTCTAGCTGTCATCGCATCGTTAACGACAACGCGGAAATCTTCTTTTCTAACGCACTGGTTTACCGTCGTATCGTAAAATCCCTGTCTAACGACGATATTAACAAAATCAGCGGTGACATGAAAGACTCCCTGAAAGAAATGTCTCTGGAAGAAATCTACTCCTATGAGAAATACGGTGAATTCATCACCCAGGAAGGCATCTCTTTCTACAACGACATCTGCGGTAAAGTTAACAGCTTCATGAACCTGTACTGCCAGAAGAACAAAGAAAACAAGAACCTGTACAAATTACAGAAACTGCACAAACAGATTCTGTGTATCGCTGATACTTCTTATGAAGTACCATACAAATTTGAGTCCGACGAAGAAGTTTACCAGTCTGTCAACGGCTTCCTGGACAACATCTCCAGCAAACACATCGTTGAACGCCTGCGTAAAATCGGTGATAACTACAATGGTTACAACCTGGACAAAATCTACATTGTGTCTAAATTCTATGAATCGGTTTCCCAGAAAACCTACCGTGACTGGGAGACTATCAACACGGCTCTGGAAATCCACTACAACAACATCCTGCCGGGCAACGGTAAATCTAAAGCAGATAAAGTTAAGAAAGCGGTAAAGAACGACTTGCAGAAATCTATCACCGAAATCAACGAACTGGTATCCAACTACAAACTGTGCAGCGACGATAACATCAAAGCTGAAACTTACATTCATGAGATCTCTCACATCCTGAACAACTTCGAAGCACAGGAACTGAAATACAACCCGGAAATCCACCTGGTTGAATCCGAGCTGAAAGCTTCTGAACTGAAGAACGTTCTGGACGTGATCATGAACGCGTTTCACTGGTGCTCAGTTTTCATGACCGAAGAACTGGTAGACAAAGACAACAACTTCTATGCTGAACTGGAGGAAATCTACGATGAAATTTACCCGGTTATCTCCCTGTACAACCTAGTACGTAACTATGTTACTCAGAAACCGTACTCTACCAAGAAAATCAAACTGAACTTCGGCATCCCGACGCTAGCGGATGGTTGGAGCAAATCTAAAGAATACTCCAACAATGCTATCATCCTGATGCGCGACAACCTGTACTACCTGGGCATTTTCAACGCGAAGAACAAACCTGATAAGAAAATCATCGAAGGCAACACCAGTGAGAACAAGGGTGACTATAAGAAAATGATCTACAACCTGCTGCCGGGTCCGAACAAAATGATCCCGAAAGTGTTTCTTTCTAGCAAAACTGGTGTTGAAACCTACAAACCATCCGCTTACATCCTGGAAGGCTACAAACAGAACAAACACATCAAATCTTCTAAAGACTTCGATATTACTTTCTGTCATGACCTGATCGACTATTTCAAAAACTGCATCGCAATCCACCCGGAATGGAAGAACTTTGGTTTCGATTTCTCCGACACCTCTACTTACGAGGACATCAGCGGGTTCTACCGTGAAGTTGAACTCCAGGGTTACAAAATCGACTGGACCTACATTTCCGAGAAAGATATCGACCTGTTACAGGAGAAAGGCCAACTGTATCTGTTTCAGATCTACAACAAAGACTTCTCTAAGAAATCCACTGGTAACGATAACCTGCACACCATGTACCTGAAAAACCTGTTCTCTGAAGAAAACCTGAAAGACATCGTACTGAAACTGAACGGCGAAGCTGAAATCTTCTTTCGTAAAAGCTCTATCAAGAACCCGATCATTCACAAGAAAGGTTCCATCCTGGTTAACCGTACTTACGAAGCCGAAGAGAAAGACCAGTTCGGCAACATCCAGATCGTTCGCAAAAACATCCCGGAAAATATCTACCAGGAACTGTACAAATATTTCAACGATAAATCTGACAAAGAACTGTCCGACGAAGCAGCTAAACTGAAAAACGTAGTTGGTCACCATGAAGCGGCTACCAACATTGTGAAAGATTACCGTTACACTTACGACAAATATTTCCTGCACATGCCGATCACCATCAACTTCAAAGCAAACAAAACTGGTTTTATCAACGACCGTATCTTGCAGTACATCGCTAAAGAAAAGGACCTGCACGTTATTGGCATCGATCGCGGTGAACGTAACCTGATCTACGTAAGCGTTATCGACACCTGTGGCAACATCGTTGAACAGAAATCTTTCAACATCGTAAACGGTTACGACTACCAGATCAAACTGAAACAGCAAGAGGGTGCGCGTCAGATTGCTCGTAAAGAGTGGAAAGAAATCGGCAAAATCAAAGAAATCAAAGAAGGCTACCTGTCACTGGTTATCCACGAAATCTCTAAAATGGTCATTAAATACAACGCAATCATCGCTATGGAAGATCTGTCCTATGGTTTCAAGAAAGGTCGCTTCAAAGTTGAACGTCAGGTTTACCAGAAATTTGAAACAATGCTGATCAACAAACTGAACTACCTGGTATTCAAAGACATCTCTATCACTGAAAACGGCGGTCTGCTGAAGGGTTACCAGCTGACCTACATTCCGGACAAACTGAAAAACGTGGGCCATCAGTGCGGTTGCATCTTCTATGTTCCGGCGGCATACACTTCCAAAATCGATCCAACCACGGGCTTCGTAAACATCTTTAAATTCAAAGACCTTACTGTTGACGCTAAACGTGAGTTCATCAAGAAATTCGATAGCATCCGTTACGACTCTGAGAAAAACCTGTTCTGTTTTACCTTCGACTACAACAACTTCATCACTCAGAACACCGTTATGAGTAAATCCTCTTGGAGCGTTTACACTTATGGTGTACGCATTAAACGTCGTTTCGTGAATGGTCGCTTTTCTAACGAATCCGACACCATCGATATCACTAAAGACATGGAAAAGACCCTGGAAATGACTGACATCAACTGGCGTGATGGCCACGACCTGCGTCAGGACATCATCGATTACGAAATTGTTCAACACATCTTCGAAATCTTCCGTCTGACCGTACAGATGCGCAACTCTCTGTCCGAACTGGAGGACCGTGACTACGATCGTCTGATCTCTCCGGTTCTCAACGAAAACAACATCTTCTACGACAGCGCTAAAGCCGGTGACGCACTGCCGAAAGACGCTGATGCGAACGGCGCTTATTGCATCGCACTGAAGGGTCTGTACGAAATTAAACAGATCACTGAAAACTGGAAAGAGGACGGCAAATTTTCCCGTGACAAACTGAAAATCTCTAACAAAGATTGGTTCGACTTCATCCAGAACAAACGCTACCTGAAACGTCCGGCTGCGACCAAAAAAGCTGGTCAGGCAAAGAAAAAAAAATGATTCTGCAGCCACCAAGAAGGCTGGCCAGGCCAAGAAGAAGAAGCTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCG  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  
<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="0" name="ORF-1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="137-160" color="#7b5ea7" type="standard" translated="1"/></Feature><Feature recentID="1" name="ORF-1'" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="137-160" color="#7b5ea7" type="standard" translated="1"/></Feature><Feature recentID="2" name="thrombin recognition and cleavage site" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="141-158" color="#7b5ea7" type="standard" translated="1"/></Feature><Feature recentID="3" name="Mad7" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="162-4019" color="#d9a400" type="standard" translated="1"/></Feature><Feature recentID="4" name="'NLS" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4030-4065" color="#7b5ea7" type="standard"/></Feature><Feature recentID="5" name="ORF-1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4030-4092" color="#d9a400" type="standard" translated="1"/></Feature><Feature recentID="6" name="6xHis affinity tag" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4072-4089" color="#7b5ea7" type="standard" translated="1"/></Feature><Feature recentID="7" name="aph(3')-Ia" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4788-5603" color="#d9a400" type="standard" translated="1"/></Feature><Feature recentID="8" name="bom" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6499-6641" color="#8b8f86" type="standard"/></Feature><Feature recentID="9" name="rop" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6743-6934" color="#d9a400" type="standard" translated="1"/></Feature><Feature recentID="10" name="lacI" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7743-8825" color="#d9a400" type="standard" translated="1"/></Feature></Features>   Ø<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/></Primers>  u<Notes>
<UUID>5878bb11-2984-5742-95ad-4fa49f2f88aa</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pet28-NLS-Mad7-NLS</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html&gt;&lt;body&gt;pet28-NLS-Mad7-NLS (F459): Ligation of Mad7 Nde-Pst* into F455-pet28-MBP-HisOpti*&lt;/body&gt;&lt;/html&gt;</Description>
<Created UTC="12:0:0">2023.3.29</Created>
<LastModified UTC="12:0:0">2023.3.29</LastModified>
<CreatedBy>DNA Cloning Service</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>&lt;html&gt;&lt;body&gt;pet28-NLS-Mad7-NLS from DNA Cloning Service, Hamburg: &lt;a href=&quot;https://dna-cloning.com/vectors/puc/pet28-NLS-Mad7-NLS&quot;&gt;https://dna-cloning.com/vectors/puc/pet28-NLS-Mad7-NLS&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;</Comments>
</Notes>
