p6i-TE9
Ligation of HPT-Xho-Mlu* into 257p7-TE9*
- Promoter 1
- 35s
- Gene 1
- hptII
- Gene 2
- MCS
- Features
- dicots, hptII mit STLS1-Intron, SfiI-Klonierung
- Other markers
- Sm/Sp
- Size
- 9,679 bp, circular
Construct
- 35S–hpt · E9 · selection cassette, 61–2,032
- MCS · 11 unique sites · at 2,042, 2,192
- SfiI ×2 · flanks the MCS · at 2,073, 2,137
- T-DNA borders · LB, RB
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| XbaI | T^CTAGA | 55 | between LB and 35S |
| XmnI (PdmI) | GAANN^NNTTC | 346 | in 35S |
| XhoI (PaeR7I) | C^TCGAG | 479 | between 35S and hpt |
| SacII (Cfr42I) | CCGC^GG | 1,475 | in hpt |
| MluI | A^CGCGT | 1,732 | between hpt and E9 |
| SbfI (SdaI) | CCTGCA^GG | 2,042 | between E9 and RB |
| SpeI | A^CTAGT | 2,081 | between E9 and RB |
| BamHI | G^GATCC | 2,087 | between E9 and RB |
| NsiI (Mph1103I) | ATGCA^T | 2,103 | between E9 and RB |
| HindIII | A^AGCTT | 2,111 | between E9 and RB |
| SalI | G^TCGAC | 2,123 | between E9 and RB |
| StuI (Eco147I) | AGG^CCT | 2,131 | between E9 and RB |
| AflII (BspTI) | C^TTAAG | 2,133 | between E9 and RB |
| BglII | A^GATCT | 2,143 | between E9 and RB |
| KpnI (Acc65I) | GGTAC^C | 2,160 | between E9 and RB |
| PmeI (MssI) | GTTT^AAAC | 2,192 | between E9 and RB |
| BsiWI (Pfl23II) | C^GTACG | 6,797 | in pVS1 |
| NheI (BmtI) | G^CTAGC | 6,913 | in pVS1 |
| BstEII (Eco91I) | G^GTNACC | 8,278 | in Sm/Sp |
Cut twice: EcoRI (749, 2,105), PstI (843, 2,042), SphI (2,048, 2,317), SmaI (490, 2,095), NcoI (858, 2,068), ClaI (2,118, 6,993), EcoRV (386, 2,704), ApaI (488, 2,154), SfiI (2,073, 2,137), FseI (4,464, 6,569), MfeI (1,030, 9,445), AgeI (7,407, 7,715), AatII (533, 2,583), BsaI (7,316, 7,736), SapI (3,636, 7,533).
No site: SacI, AscI, PacI, SwaI, AvrII, BstBI, HpaI, BsrGI, PvuII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 61 | 476 | 416 | + |
| hpt | Selection marker misc_feature | 506 | 923 | 418 | + |
| STLS1 | Other misc_feature | 924 | 1,112 | 189 | + |
| hpt | Selection marker misc_feature | 1,113 | 1,720 | 608 | + |
| 'E9 | Terminator misc_feature | 1,742 | 2,032 | 291 | + |
| RB | T-DNA border misc_feature | 2,208 | 2,231 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 2,766 | 3,463 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 4,012 | 7,717 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 7,901 | 8,911 | 1,011 | + |
| LB | T-DNA border misc_feature | 9,432 | 9,455 | 24 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.