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DNA Cloning Service
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DNA Cloning Service: cloning and sequenced plant vectors for labs worldwide.

Cloning into any vector, gene synthesis, site-directed mutagenesis and CRISPR/Cas vector construction. 416 sequenced binary, pUC, RNAi, CRISPR/Cas and E. coli expression vectors for plant transformation, with GenBank files and maps, ready to ship.

or browse Binaries, pUC and E. coli expression, RNAi, CRISPR/Cas and base editors.

2468101214Cas9WT'pVS1Ubi-intSm/SpP2x35sColE1hptIIIntron'hptIIU6OsCas9-intE9NOS-TintintgRNA scaffoldNLSRBLBB330p6i2xoR-Ucas15,473 bp
B330p6i2xoR-Ucas: Cas9 nuclease binary vector for monocots, 15,473 bp. Maps on this site are drawn from the GenBank file of each vector.
183BinariesBinary vectors for Agrobacterium-mediated transformation of monocots and dicots, with bar, hpt, npt or dhfr selection.115pUC and E. coli expressionSfiI-based pUC cloning vectors with promoter and terminator cassettes, markers and building blocks, and pET vectors that express Cas nucleases and base editors in E. coli.31RNAiHairpin constructs with intron or spacer for RNA interference in monocots and dicots.87CRISPR/CasCas9 nuclease and nickase binary vectors with U6 promoters from Arabidopsis or rice for one or several targets.10Base editor vectorsA nicked Cas fused to a cytidine deaminase (CBE): C-to-T editing without a double-strand break.19Multiple expression cassettesTwo or more empty expression cassettes, each a promoter and a terminator around a cloning site: several genes in one construct.

How an order works

  1. 1
    Pick a vector or describe the construct. Every listed vector has its GenBank file, so you can check the map before you write.
  2. 2
    Send the name or the sequence to info@dna-cloning.com. For cloning, gene synthesis and mutagenesis we send you a quote, usually within a day.
  3. 3
    We ship on invoice. DNA or glycerol stock, with sequence, map and primers. Kits from the shop go the same way.

Cloning into any vector

Send us the plasmid, or the sequence to amplify, and the vector you want it in. You receive the construct as glycerol stock or DNA together with the sequence, a map and the primers used. Restriction cloning, seamless cloning and assembly of up to five fragments.

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Gene synthesis

Genes synthesised from primers and cloned into a basic pUC vector. Codon usage optimised for monocots, dicots or both, Kozak variants for high, medium and low expression, and an intron to stop expression in Agrobacterium. Our synthetic GUS gene with the StLS1 intron is one example.

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Site-directed mutagenesis

Point mutations or a complex mutation in your plasmid, up to four point mutations in one go. You receive the mutated plasmid with its sequence and map.

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CRISPR/Cas vector construction

Binary vectors with a codon-optimised Cas9 nuclease or nickase and the gRNA under the U6 promoter from Arabidopsis or rice. We clone one target site, or several: up to eight double target sites into one vector. See the CRISPR/Cas vectors.

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Sequenced plant transformation vectors

416 binary, pUC, RNAi and CRISPR/Cas vectors for monocots and dicots, each with its GenBank file and map, so you can check the construct before you order. If the vector you need is not in the list, we build it.

From 250 € per vector; CRISPR/Cas vectors 400 €, multiple expression cassette vectors 400 €, base editor vectors 500 €: browse the vectors.

Not sure which service fits? Describe the construct you need and we send you a quote.

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Kits and reagents

Everything we use at the bench ourselves, shipped on invoice to labs in Germany and Europe.