DNA Cloning Service in Hamburg: cloning and sequenced plant vectors.

Cloning into any vector, gene synthesis, site-directed mutagenesis and CRISPR/Cas vector construction. 416 sequenced binary, pUC, RNAi, CRISPR/Cas and E. coli expression vectors for plant transformation, with GenBank files and maps, ready to ship.

or browse Binaries, pUC and E. coli expression, RNAi, CRISPR/Cas, or see the price list.

B330p6i2xoR-Ucas: Cas9 nuclease binary vector for monocots, 15,473 bp. Maps on this site are drawn from the GenBank file of each vector.

183BinariesBinary vectors for Agrobacterium-mediated transformation of monocots and dicots, with bar, hpt, npt or dhfr selection.115pUC and E. coli expressionSfiI-based pUC cloning vectors with promoter and terminator cassettes, markers and building blocks, and pET vectors that express Cas nucleases and base editors in E. coli.31RNAiHairpin constructs with intron or spacer for RNA interference in monocots and dicots.87CRISPR/CasCas9 nuclease and nickase binary vectors with U6 promoters from Arabidopsis or rice for one or several targets.

How an order works

1
Pick a vector or describe the construct. Every listed vector has its GenBank file, so you can check the map before you write.
2
Send the name or the sequence to info@dna-cloning.com. For cloning we quote from the price list, usually within a day.
3
We ship on invoice. DNA or glycerol stock, with sequence, map and primers. Kits from the shop go the same way.

Cloning into any vector

Send us the plasmid, or the sequence to amplify, and the vector you want it in. You receive the construct as glycerol stock or DNA together with the sequence, a map and the primers used. Restriction cloning, seamless cloning and assembly of up to five fragments.

From 250 € per fragment, sequencing not included: cloning prices.

Gene synthesis

Genes synthesised from primers and cloned into a basic pUC vector. Codon usage optimised for monocots, dicots or both, Kozak variants for high, medium and low expression, and an intron to stop expression in Agrobacterium. Our synthetic GUS gene with the StLS1 intron is one example.

250 € for genes below 625 bp, 0,35 € per base above: prices.

Site-directed mutagenesis

Point mutations or a complex mutation in your plasmid, up to four point mutations in one go. You receive the mutated plasmid with its sequence and map.

300 € for one point mutation, primers and sequencing not included: mutagenesis prices.

CRISPR/Cas vector construction

Binary vectors with a codon-optimised Cas9 nuclease or nickase and the gRNA under the U6 promoter from Arabidopsis or rice. We clone one target site, or several: up to eight double target sites into one vector. See the CRISPR/Cas vectors.

350 € for one target site, primers and sequencing included: CRISPR prices.

Sequenced plant transformation vectors

416 binary, pUC, RNAi and CRISPR/Cas vectors for monocots and dicots, each with its GenBank file and map, so you can check the construct before you order. If the vector you need is not in the list, we build it.

250 € per vector, Cas9 vectors 400 €: browse the vectors.

Not sure which service fits? Write to us with the construct you need; we quote from the price list.

Kits and reagents

Everything we use at the bench ourselves, shipped on invoice to labs in Germany and Europe.