p7oUH-LH-ocs B420
Ligation of pOCS-BM* into B417p7oUH-LH*
- Promoter 1
- UbiHa
- Gene 1
- bar
- Gene 2
- MCS
- Features
- dicots
- Other markers
- Sm/Sp
- Size
- 9,311 bp, circular
- Record date
- 22-JAN-2016
Construct
- UbiZm–bar · TE9 · selection cassette, 316–1,712
- MCS · 8 unique sites · at 1,722, 1,803
- SfiI ×2 · flanks the MCS · at 1,753, 2,017
- T-DNA borders · LB, RB
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| XhoI (PaeR7I) | C^TCGAG | 653 | between UbiZm and bar |
| AarI | CACCTGC(4/8) | 1,194 | in bar |
| XmnI (PdmI) | GAANN^NNTTC | 1,414 | between bar and TE9 |
| SbfI (SdaI) | CCTGCA^GG | 1,722 | between TE9 and Tocs |
| NcoI | C^CATGG | 1,748 | between TE9 and Tocs |
| SpeI | A^CTAGT | 1,761 | between TE9 and Tocs |
| BamHI | G^GATCC | 1,767 | between TE9 and Tocs |
| SmaI (XmaI, TspMI) | CCC^GGG | 1,775 | between TE9 and Tocs |
| EcoRI | G^AATTC | 1,785 | between TE9 and Tocs |
| HindIII | A^AGCTT | 1,791 | between TE9 and Tocs |
| SalI | G^TCGAC | 1,803 | between TE9 and Tocs |
| StuI (Eco147I) | AGG^CCT | 2,011 | between Tocs and RB |
| BglII | A^GATCT | 2,023 | between Tocs and RB |
| KpnI (Acc65I) | GGTAC^C | 2,040 | between Tocs and RB |
| PmeI (MssI) | GTTT^AAAC | 2,072 | between Tocs and RB |
| AatII (ZraI) | GACGT^C | 2,463 | between RB and ColE1 |
| EcoRV (Eco32I) | GAT^ATC | 2,584 | between RB and ColE1 |
| BbsI (BpiI) | GAAGAC(2/6) | 3,873 | between bom and pVS1 |
| BsiWI (Pfl23II) | C^GTACG | 6,677 | in pVS1 |
| NheI (BmtI) | G^CTAGC | 6,793 | in pVS1 |
| ClaI (BspDI) | AT^CGAT | 6,873 | in pVS1 |
| BstEII (Eco91I) | G^GTNACC | 8,158 | in Sm/Sp |
Cut twice: XbaI (303, 384), PstI (1,722, 1,783), SphI (1,728, 2,197), NdeI (3,578, 5,337), MluI (1,412, 1,797), SfiI (1,753, 2,017), FseI (4,344, 6,449), MfeI (14, 1,011), AgeI (7,287, 7,595), AflII (320, 2,013), SacII (1,294, 1,311), SapI (3,516, 7,413).
No site: SacI, AscI, PacI, SwaI, AvrII, BstBI, HpaI, NsiI, BsrGI, PvuII.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| UbiZm | Promoter misc_feature | 316 | 651 | 336 | + |
| cat | Site misc_signal | 433 | 435 | 3 | + |
| cat | Site misc_signal | 436 | 438 | 3 | + |
| cat | Site misc_signal | 469 | 471 | 3 | + |
| cat | Site misc_signal | 498 | 500 | 3 | + |
| cat | Site misc_signal | 509 | 511 | 3 | + |
| cat | Site misc_signal | 530 | 532 | 3 | + |
| cat | Site misc_signal | 533 | 535 | 3 | + |
| tata | Site misc_signal | 538 | 541 | 4 | + |
| mRNA | Coding sequence CDS | 569 | 651 | 83 | + |
| bar | Selection marker CDS | 671 | 904 | 234 | + |
| int | Site misc_feature | 905 | 1,093 | 189 | + |
| bar | Selection marker CDS | 1,094 | 1,409 | 316 | + |
| TE9 | Terminator misc_feature | 1,422 | 1,712 | 291 | + |
| Tocs | Terminator misc_feature | 1,808 | 2,003 | 196 | + |
| RB | T-DNA border misc_feature | 2,088 | 2,111 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 2,646 | 3,343 | 698 | + |
| bom | Origin / backbone misc_feature | 3,380 | 3,639 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 3,892 | 7,597 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 7,781 | 8,791 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.