p9o-UbiAt-GusiX C786
Ligation of GusiX-Bam-Hind* into C599p9o-LH-U10-ocs*
- Promoter 1
- 35s
- Gene 1
- nptII
- Promoter 2
- Ubq10
- Gene 2
- gusX
- Features
- dicots
- Other markers
- NptIIo, Sm/Sp
- Size
- 13,103 bp, circular
- Record date
- 26-AUG-2020
Construct
- P35S–NptIIo · TE9 · selection cassette, 309–2,038
- Pro-Ubq10At–GusX · OCS · payload cassette, 2,087–5,795
- MCS · 4 unique sites · at 5,803, 5,864
- SfiI ×2 · flanks the Pro-Ubq10At–GusX cassette · at 2,079, 5,809
- T-DNA borders · LB, RB
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| XhoI (PaeR7I) | C^TCGAG | 727 | between P35S and NptIIo |
| PstI | CTGCA^G | 2,048 | between TE9 and Pro-Ubq10At |
| SbfI (SdaI) | CCTGCA^GG | 2,048 | between TE9 and Pro-Ubq10At |
| NcoI | C^CATGG | 2,074 | between TE9 and Pro-Ubq10At |
| EcoRI | G^AATTC | 2,747 | in Pro-Ubq10At |
| SpeI | A^CTAGT | 3,563 | between Pro-Ubq10At and GusX |
| BamHI | G^GATCC | 3,569 | between Pro-Ubq10At and GusX |
| HpaI (KspAI) | GTT^AAC | 3,880 | in GusX |
| BstBI (Bsp119I) | TT^CGAA | 3,924 | in GusX |
| HindIII | A^AGCTT | 5,583 | between GusX and OCS |
| StuI (Eco147I) | AGG^CCT | 5,803 | between OCS and RB |
| AflII (BspTI) | C^TTAAG | 5,805 | between OCS and RB |
| KpnI (Acc65I) | GGTAC^C | 5,832 | between OCS and RB |
| PmeI (MssI) | GTTT^AAAC | 5,864 | between OCS and RB |
| BsiWI (Pfl23II) | C^GTACG | 10,469 | in pVS1 |
| NheI (BmtI) | G^CTAGC | 10,585 | in pVS1 |
| ClaI (BspDI) | AT^CGAT | 10,665 | in pVS1 |
Cut twice: SalI (2,925, 5,595), SphI (2,054, 5,989), SacI (1,255, 3,180), NdeI (7,370, 9,129), BglII (3,492, 5,815), ApaI (828, 5,826), MluI (1,738, 5,589), SfiI (2,079, 5,809), AgeI (11,079, 11,387).
No site: SmaI, AscI, PacI, SwaI, AvrII, NsiI, BsrGI, SacII, PvuII.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P35S | Promoter misc_feature | 309 | 724 | 416 | + |
| NptIIo | Selection marker CDS | 745 | 1,043 | 299 | + |
| splice | Site misc_feature | 1,040 | 1,043 | 4 | + |
| int | Site CDS | 1,044 | 1,230 | 187 | + |
| splice | Site misc_feature | 1,229 | 1,232 | 4 | + |
| NptIIo | Selection marker CDS | 1,231 | 1,734 | 504 | + |
| TE9 | Terminator misc_feature | 1,748 | 2,038 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 2,087 | 3,562 | 1,476 | + |
| TATA | Site misc_feature | 3,158 | 3,161 | 4 | + |
| Ubi cds 5 | Promoter misc_feature | 3,191 | 3,254 | 64 | + |
| íntron | Other misc_feature | 3,257 | 3,560 | 304 | + |
| GusX | Coding sequence CDS | 3,584 | 4,198 | 615 | + |
| ''Intron | Site misc_feature | 4,199 | 4,387 | 189 | + |
| GusX | Coding sequence CDS | 4,388 | 5,583 | 1,196 | + |
| OCS | Terminator misc_feature | 5,600 | 5,795 | 196 | + |
| RB | T-DNA border misc_feature | 5,880 | 5,903 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 6,438 | 7,135 | 698 | + |
| bom | Origin / backbone misc_feature | 7,172 | 7,431 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 7,684 | 11,389 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 11,573 | 12,583 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.