pGFPU10-35s-ocs-LH C757
Ligation of GFP-Xho-BspE* into B469pRedU10-35s-ocs-LH*
- Promoter 1
- Ubq10
- Gene 1
- EGFP
- Promoter 2
- 35s
- Features
- dicots
- Other markers
- Sm/Sp
- Size
- 11,034 bp, circular
- Record date
- 26-AUG-2020
Construct
- Pro-Ubq10At–GFP · TE9 · payload cassette, 309–3,007
- 35S-Pro–(empty)–OCS · empty expression cassette, 3,062–3,726
- MCS · 5 unique sites · at 3,483, 3,520
- SfiI ×2 · flanks the 35S-Pro–(empty)–OCS cassette · at 3,048, 3,740
- T-DNA borders · LB, RB
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 1,402 | in Pro-Ubq10At |
| XhoI (PaeR7I) | C^TCGAG | 1,786 | between Pro-Ubq10At and GFP |
| BstBI (Bsp119I) | TT^CGAA | 2,144 | in GFP |
| SbfI (SdaI) | CCTGCA^GG | 3,017 | between TE9 and 35S-Pro |
| NcoI | C^CATGG | 3,043 | between TE9 and 35S-Pro |
| SpeI | A^CTAGT | 3,483 | in 35S-Pro |
| BamHI | G^GATCC | 3,490 | between 35S-Pro and OCS |
| SmaI (XmaI, TspMI) | CCC^GGG | 3,498 | between 35S-Pro and OCS |
| HindIII | A^AGCTT | 3,514 | between 35S-Pro and OCS |
| MluI | A^CGCGT | 3,520 | between 35S-Pro and OCS |
| StuI (Eco147I) | AGG^CCT | 3,734 | between OCS and RB |
| AflII (BspTI) | C^TTAAG | 3,736 | between OCS and RB |
| ApaI (PspOMI) | GGGCC^C | 3,757 | between OCS and RB |
| PmeI (MssI) | GTTT^AAAC | 3,795 | between OCS and RB |
| AatII (ZraI) | GACGT^C | 4,186 | between RB and ColE1 |
| BsiWI (Pfl23II) | C^GTACG | 8,400 | in pVS1 |
| NheI (BmtI) | G^CTAGC | 8,516 | in pVS1 |
| BstEII (Eco91I) | G^GTNACC | 9,881 | in Sm/Sp |
Cut twice: EcoRI (969, 3,508), SalI (1,147, 3,526), PstI (3,017, 3,506), SphI (3,023, 3,920), KpnI (2,025, 3,763), NdeI (5,301, 7,060), BglII (1,714, 3,746), ClaI (2,375, 8,596), SfiI (3,048, 3,740), FseI (6,067, 8,172), MfeI (14, 2,289), AgeI (9,010, 9,318), XmnI (570, 3,350), BsaI (8,919, 9,339).
No site: AscI, PacI, SwaI, AvrII, HpaI, NsiI, BsrGI, SacII, PvuII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| Pro-Ubq10At | Promoter misc_feature | 309 | 1,784 | 1,476 | + |
| Ubi cds 5 | Promoter misc_feature | 1,413 | 1,476 | 64 | + |
| íntron | Other misc_feature | 1,479 | 1,782 | 304 | + |
| GFP | Coding sequence CDS | 1,804 | 2,182 | 379 | + |
| ST-LS1 | Coding sequence CDS | 2,183 | 2,371 | 189 | + |
| GFP | Coding sequence CDS | 2,372 | 2,709 | 338 | + |
| TE9 | Terminator misc_feature | 2,727 | 3,007 | 281 | + |
| 35S-Pro | Promoter misc_feature | 3,062 | 3,484 | 423 | + |
| TATA | Site CDS | 3,450 | 3,456 | 7 | + |
| OCS | Terminator misc_feature | 3,531 | 3,726 | 196 | + |
| RB | T-DNA border misc_feature | 3,811 | 3,834 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 4,369 | 5,066 | 698 | + |
| bom | Origin / backbone misc_feature | 5,103 | 5,362 | 260 | + |
| pVS1 | Origin / backbone misc_feature | 5,615 | 9,320 | 3,706 | + |
| Sm/Sp | Selection marker CDS | 9,504 | 10,514 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.
Used in
- A chromosome 2 locus controls transient Agrobacterium-mediated GFP expression in rose petals – Ho NH, Linde M, Debener T, Plant Cell Reports 2026; 45:278
- Genome-Wide Analysis of the WRKY Transcription Factor Family in Roses and Their Putative Role in Defence Signalling in the Rose–Blackspot Interaction – Domes HS, Debener T, Plants 2024; 13:1066
- Unraveling the genetic basis of Rhizobium rhizogenes-mediated transformation and hairy root formation in rose using a genome-wide association study – Rüter P, Debener T, Winkelmann T, Plant Cell Reports 2024; 43:300
- A highly mutable GST is essential for bract colouration in Euphorbia pulcherrima Willd. Ex Klotsch – Vilperte V, Boehm R, Debener T, BMC Genomics 2021; 22:208