pHC-nCas9 F189
Ligation of F188p7D35s-C9Nick* into HC5-Spe-Xma*
- Gene 2
- Cas9 nick
- Size
- 5,909 bp, circular
- Record date
- 17-AUG-2022
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SpeI | A^CTAGT | 69 | between O1 and Cas9WT |
| BamHI | G^GATCC | 75 | between O1 and Cas9WT |
| SacI (Ecl136II) | GAGCT^C | 131 | in Cas9WT |
| BglII | A^GATCT | 382 | in Cas9WT |
| AatII (ZraI) | GACGT^C | 673 | in Cas9WT |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 1,285 | in Cas9WT |
| NcoI | C^CATGG | 1,554 | in Cas9WT |
| StuI (Eco147I) | AGG^CCT | 2,851 | in Cas9WT |
| SphI (PaeI) | GCATG^C | 3,398 | in Cas9WT |
| SmaI (XmaI, TspMI) | CCC^GGG | 4,289 | between Cas9WT and O2 |
| XmnI (PdmI) | GAANN^NNTTC | 4,569 | in ORF-1 |
| PvuI | CGAT^CG | 4,800 | in ORF-1 |
Cut twice: EcoRV (1,973, 4,361), SacII (626, 2,723).
No site: EcoRI, HindIII, XbaI, SalI, PstI, KpnI, NotI, XhoI, NdeI, NheI, ClaI, ApaI, MluI, AscI, PacI, SfiI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, NsiI, BsrGI, EagI, BstEII, BsaI, BbsI, BsmBI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| O1 | Site CDS | 30 | 50 | 21 | + |
| 'Cas9WT | Coding sequence CDS | 120 | 4,286 | 4,167 | + |
| D10A | Site misc_feature | 160 | 160 | 1 | + |
| NLS | Site misc_feature | 4,236 | 4,283 | 48 | + |
| O2 | Site CDS | 4,307 | 4,327 | 21 | + |
| ORF-1 | Coding sequence CDS | 4,382 | 5,242 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 5,245 | 5,909 | 665 | + |
| LC | Site misc_feature | 5,467 | 5,467 | 1 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.