p35s-CheriD-ocs B893
Ligation of cheri-Mlu-Sal* into B892p35S-CherD-ocs*
- Promoter 2
- 35s
- Gene 2
- mCherry
- Features
- dicots
- Other markers
- Amp
- Size
- 4,483 bp, circular
- Record date
- 04-OCT-2017
Construct
- 35S-Pro–mCherry · OCS · payload cassette, 144–1,693
- MCS · 8 unique sites · at 103, 140
- SfiI ×2 · flanks the 35S-Pro–mCherry cassette · at 118, 1,707
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 103 | between ColE1 and 35S-Pro |
| XbaI | T^CTAGA | 105 | between ColE1 and 35S-Pro |
| NcoI | C^CATGG | 113 | between ColE1 and 35S-Pro |
| NotI | GC^GGCCGC | 119 | between ColE1 and 35S-Pro |
| EagI (Eco52I) | C^GGCCG | 119 | between ColE1 and 35S-Pro |
| SpeI | A^CTAGT | 126 | between ColE1 and 35S-Pro |
| BamHI | G^GATCC | 132 | between ColE1 and 35S-Pro |
| SmaI (XmaI, TspMI) | CCC^GGG | 140 | between ColE1 and 35S-Pro |
| EcoRV (Eco32I) | GAT^ATC | 472 | in 35S-Pro |
| HindIII | A^AGCTT | 567 | between 35S-Pro and mCherry' |
| MluI | A^CGCGT | 573 | between 35S-Pro and mCherry' |
| HpaI (KspAI) | GTT^AAC | 670 | in mCherry' |
| BstEII (Eco91I) | G^GTNACC | 916 | in mCherry' |
| MfeI (MunI) | C^AATTG | 1,074 | in Int |
| AarI | CACCTGC(4/8) | 1,144 | in Int |
| SacII (Cfr42I) | CCGC^GG | 1,168 | in mCherry |
| SalI | G^TCGAC | 1,493 | between mCherry and OCS |
| BsmBI (Esp3I) | CGTCTC(1/5) | 1,513 | in OCS |
| StuI (Eco147I) | AGG^CCT | 1,701 | between OCS and Amp |
| BglII | A^GATCT | 1,713 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 1,724 | between OCS and Amp |
| KpnI (Acc65I) | GGTAC^C | 1,730 | between OCS and Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 3,230 | in Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 4,301 | between ColE1 and 35S-Pro |
Cut twice: SfiI (118, 1,707), AflII (851, 1,703), PvuI (1,882, 2,927), PvuII (1,851, 4,364), XmnI (432, 2,696).
No site: EcoRI, PstI, SphI, XhoI, NdeI, NheI, ClaI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, AgeI, BstBI, NsiI, BsrGI, AatII.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S-Pro | Promoter misc_feature | 144 | 562 | 419 | + |
| Minimal promotor | Site misc_feature | 473 | 562 | 90 | + |
| mCherry' | Coding sequence CDS | 590 | 969 | 380 | + |
| Int | Site CDS | 987 | 1,156 | 170 | + |
| 'mCherry | Coding sequence CDS | 1,168 | 1,489 | 322 | + |
| OCS | Terminator misc_feature | 1,498 | 1,693 | 196 | + |
| Amp | Selection marker misc_feature | 2,509 | 3,369 | 861 | + |
| ColE1 | Origin / backbone CDS | 3,412 | 4,128 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.