p35s-LmVenus D323
Ligation of mVenus-Mlu-Sal* into B629p35s-LGFP*
- Promoter 1
- 35s
- Gene 1
- mVenus
- Features
- dicots and monocots
- Other markers
- Amp
- Size
- 4,363 bp, circular
- Record date
- 25-AUG-2020
Construct
- 35S-Pro–ORF-1 · OCS · payload cassette, 106–1,547
- MCS · 8 unique sites · at 527, 570
- SfiI ×2 · flanks the 35S-Pro–ORF-1 cassette · at 92, 1,561
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 77 | between ColE1 and 35S-Pro |
| XbaI | T^CTAGA | 79 | between ColE1 and 35S-Pro |
| NcoI | C^CATGG | 87 | between ColE1 and 35S-Pro |
| NotI | GC^GGCCGC | 93 | between ColE1 and 35S-Pro |
| EagI (Eco52I) | C^GGCCG | 93 | between ColE1 and 35S-Pro |
| EcoRV (Eco32I) | GAT^ATC | 434 | in 35S-Pro |
| SpeI | A^CTAGT | 527 | in 35S-Pro |
| BamHI | G^GATCC | 534 | between 35S-Pro and ORF-1 |
| SmaI (XmaI, TspMI) | CCC^GGG | 542 | between 35S-Pro and ORF-1 |
| PstI | CTGCA^G | 550 | between 35S-Pro and ORF-1 |
| EcoRI | G^AATTC | 552 | between 35S-Pro and ORF-1 |
| HindIII | A^AGCTT | 558 | between 35S-Pro and ORF-1 |
| MluI | A^CGCGT | 564 | between 35S-Pro and ORF-1 |
| BsrGI (Bsp1407I) | T^GTACA | 570 | between 35S-Pro and ORF-1 |
| BstEII (Eco91I) | G^GTNACC | 809 | in ORF-1 |
| SalI | G^TCGAC | 1,347 | between ORF-1 and OCS |
| BsmBI (Esp3I) | CGTCTC(1/5) | 1,367 | in OCS |
| StuI (Eco147I) | AGG^CCT | 1,555 | between OCS and Amp |
| BglII | A^GATCT | 1,567 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 1,578 | between OCS and Amp |
| KpnI (Acc65I) | GGTAC^C | 1,584 | between OCS and Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 3,084 | in Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 4,155 | between ColE1 and 35S-Pro |
Cut twice: SfiI (92, 1,561), AflII (759, 1,557), PvuI (1,736, 2,781), PvuII (1,705, 4,218), XmnI (394, 2,550).
No site: SphI, XhoI, NdeI, NheI, ClaI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, NsiI, SacII, AatII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S-Pro | Promoter misc_feature | 106 | 528 | 423 | + |
| TATA | Site CDS | 494 | 500 | 7 | + |
| ORF-1 | Coding sequence CDS | 627 | 1,346 | 720 | + |
| OCS | Terminator misc_feature | 1,352 | 1,547 | 196 | + |
| Amp | Selection marker misc_feature | 2,363 | 3,223 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 3,266 | 3,982 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.