p35s-YFPD B726
Ligation of cit-DCS-Mlu-Sal* into B589p35s-mGFPD-ocs*
- Promoter 2
- 35s
- Gene 2
- mCitrine
- Features
- dicots
- Other markers
- Amp
- Size
- 4,303 bp, circular
- Record date
- 26-JAN-2016
Construct
- P35S–yfp–cit · Tocs · payload cassette, 144–1,513
- MCS · 8 unique sites · at 103, 140
- SfiI ×2 · flanks the P35S–yfp–cit cassette · at 118, 1,527
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 103 | between ColE1 and P35S |
| XbaI | T^CTAGA | 105 | between ColE1 and P35S |
| NcoI | C^CATGG | 113 | between ColE1 and P35S |
| NotI | GC^GGCCGC | 119 | between ColE1 and P35S |
| EagI (Eco52I) | C^GGCCG | 119 | between ColE1 and P35S |
| SpeI | A^CTAGT | 126 | between ColE1 and P35S |
| BamHI | G^GATCC | 132 | between ColE1 and P35S |
| SmaI (XmaI, TspMI) | CCC^GGG | 140 | between ColE1 and P35S |
| EcoRV (Eco32I) | GAT^ATC | 472 | in P35S |
| HindIII | A^AGCTT | 567 | between P35S and yfp |
| MluI | A^CGCGT | 573 | between P35S and yfp |
| ClaI (BspDI) | AT^CGAT | 975 | in cit |
| SalI | G^TCGAC | 1,313 | between yfp and Tocs |
| BsmBI (Esp3I) | CGTCTC(1/5) | 1,333 | in Tocs |
| StuI (Eco147I) | AGG^CCT | 1,521 | between Tocs and Amp |
| AflII (BspTI) | C^TTAAG | 1,523 | between Tocs and Amp |
| BglII | A^GATCT | 1,533 | between Tocs and Amp |
| ApaI (PspOMI) | GGGCC^C | 1,544 | between Tocs and Amp |
| KpnI (Acc65I) | GGTAC^C | 1,550 | between Tocs and Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 3,050 | in Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 4,121 | between ColE1 and P35S |
Cut twice: SfiI (118, 1,527), PvuI (1,702, 2,747), PvuII (1,671, 4,184), XmnI (432, 2,516).
No site: EcoRI, PstI, SphI, XhoI, NdeI, NheI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, NsiI, BsrGI, SacII, AatII, BstEII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| P35S | Promoter misc_feature | 144 | 562 | 419 | + |
| yfp | Coding sequence CDS | 590 | 1,309 | 720 | + |
| cit | Coding sequence CDS | 972 | 1,309 | 338 | + |
| Tocs | Terminator misc_feature | 1,318 | 1,513 | 196 | + |
| Amp | Selection marker misc_feature | 2,329 | 3,189 | 861 | + |
| ColE1 | Origin / backbone CDS | 3,232 | 3,948 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.