pAM
AB-pst rotated to position 2533
- Gene 2
- MCS
- Features
- basic cloning vector
- Other markers
- Amp
- Size
- 2,958 bp, circular
- Record date
- 06-JUL-2010
Construct
- MCS · 18 unique sites · at 403, 505
- SfiI ×2 · flanks the MCS · at 418, 483
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 118 | between ColE1 and LacZ |
| SacI (Ecl136II) | GAGCT^C | 403 | in LacZ |
| XbaI | T^CTAGA | 405 | in LacZ |
| AflII (BspTI) | C^TTAAG | 415 | in LacZ |
| StuI (Eco147I) | AGG^CCT | 421 | in LacZ |
| SpeI | A^CTAGT | 426 | in LacZ |
| BamHI | G^GATCC | 432 | in LacZ |
| SmaI (XmaI, TspMI) | CCC^GGG | 440 | in LacZ |
| PstI | CTGCA^G | 448 | in LacZ |
| EcoRI | G^AATTC | 450 | in LacZ |
| HindIII | A^AGCTT | 456 | in LacZ |
| MluI | A^CGCGT | 462 | in LacZ |
| SalI | G^TCGAC | 468 | in LacZ |
| NotI | GC^GGCCGC | 475 | in LacZ |
| EagI (Eco52I) | C^GGCCG | 475 | in LacZ |
| NcoI | C^CATGG | 481 | in LacZ |
| BglII | A^GATCT | 489 | in LacZ |
| ApaI (PspOMI) | GGGCC^C | 499 | in LacZ |
| KpnI (Acc65I) | GGTAC^C | 505 | in LacZ |
| XmnI (PdmI) | GAANN^NNTTC | 1,471 | in Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 2,005 | in Amp |
Cut twice: SfiI (418, 483), PvuI (657, 1,702), PvuII (181, 626).
No site: SphI, XhoI, NdeI, NheI, ClaI, EcoRV, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, NsiI, BsrGI, SacII, AatII, BstEII, BbsI, BsmBI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LacZ | Coding sequence CDS | 343 | 705 | 363 | + |
| Amp | Selection marker misc_feature | 1,284 | 2,144 | 861 | + |
| ColE1 | Origin / backbone CDS | 2,187 | 2,903 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.