pENTA2 A107
EntA2* Circularized
- Features
- basic entry vector with ampecillin for bacterial selection
- Size
- 2,295 bp, circular
- Record date
- 20-JUN-2013
Construct
- MCS · 7 unique sites · at 295, 345
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| AatII (ZraI) | GACGT^C | 12 | between amp and attL1 |
| EcoRV (Eco32I) | GAT^ATC | 178 | between amp and attL1 |
| PvuII | CAG^CTG | 183 | between amp and attL1 |
| SpeI | A^CTAGT | 295 | between attL1 and frame |
| BamHI | G^GATCC | 302 | between attL1 and frame |
| SmaI (XmaI, TspMI) | CCC^GGG | 311 | between attL1 and frame |
| EcoRI | G^AATTC | 323 | between attL1 and frame |
| HindIII | A^AGCTT | 330 | between attL1 and frame |
| MluI | A^CGCGT | 337 | between attL1 and frame |
| SalI | G^TCGAC | 345 | between attL1 and frame |
| ApaI (PspOMI) | GGGCC^C | 457 | between attL2 and ColE1 |
| AflII (BspTI) | C^TTAAG | 462 | between attL2 and ColE1 |
| NheI (BmtI) | G^CTAGC | 511 | between attL2 and ColE1 |
| BsaI (Eco31I) | GGTCTC(1/5) | 1,446 | in amp |
| PvuI | CGAT^CG | 1,755 | in amp |
| XmnI (PdmI) | GAANN^NNTTC | 1,984 | in amp |
Cut twice: PstI (320, 1,630), BsrGI (274, 365).
No site: XbaI, SphI, KpnI, SacI, NotI, XhoI, NcoI, NdeI, BglII, ClaI, AscI, PacI, SfiI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, StuI, NsiI, EagI, SacII, BstEII, BbsI, BsmBI, SapI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| attL1 | Other misc_feature | 192 | 291 | 100 | + |
| frame | Site misc_signal | 352 | 354 | 3 | + |
| attL2 | Other misc_feature | 353 | 452 | 100 | + |
| ColE1 | Origin / backbone misc_feature | 553 | 1,264 | 712 | + |
| amp | Selection marker CDS | 1,312 | 2,172 | 861 | − |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.